1. Cysteine proteinase inhibitors stefin A and stefin B in operable carcinoma of the head and neck : Inhibitorji cisteinskih proteinaz stefin A in stefin B pri operabilnem karcinomu glave in vratuPrimož Strojan, Marjan Budihna, Alojz Šmid, Branka Svetic, Ivan Vrhovec, Janko Kos, Janez Škrk, 2002, original scientific article Abstract: Purpose. To evaluate the significance of cysteine proteinase inhibitors stefins (Stefs) A and B for a treatment decision and prognosis in operable squamous cell carcinoma of the head and neck (SCCHN). Patients and methods. Stefs A and B concentrations were determined immunobiochemically using ELISAs in cytosols prepared from the tumor and adjacent normal mucosa from 91 patients with operable SCCHN. The median follow-up period of patients alive atthe close-out date was 5.8 years (range, 5-9.3 years). Results. Stef A concentrations were significantly higher in tumor compared to normal mucosa (FM.05). When a subgroup with clinically palpable nodes) at presentation was taken into consideration (n=57), a significant difference in Stef A (P=0.03) and Stef B (P=0.02) concentrations between those with negative and positive necks, as determined on histopathological examination, was observed. On the univariate survival analysis, higher Stefsć concentrations turned to be prognostically advantageous. Stef A proved its independent prognostic significance also on multivariate setting. Conclusions. With the capability todifferentiate between the pN0- and pN+-stages of the disease in the patientsoriginally presented as node-positive, Stefs A and B could be useful markers when deciding on the extent of neck surgery. In addition, both Stefs proved to be reliable prognosticators for survival in patients with operable SCCHN. Published in DiRROS: 31.01.2024; Views: 119; Downloads: 29 Full text (114,91 KB) |
2. Cathepsin D and plasminogen activator inhibitor type 1 in normal, benign and alignant ovarian tissues : a preliminary reportMarina Šprem, Damir Babić, Marija Abramić, Duško Miličić, Ivan Vrhovec, Janez Škrk, Maja Osmak, 2000, original scientific article Abstract: Background. The aim of the present study was to determine the concentration ofcathepsin D (Cath D) and plasminogen activator inhibitor type 1 (PAI-1) in normal ovarian tissues, benign and malignant ovarian tumor tissues, and to asses relationship between Cath D and PAI-1 content, and some clinical and pathohistological parameters. Materials and methods. Cath D contents and PAI-1concentrations were determined (using immunoradiometric ELSA-Cath D assayand commercial IMUDIND R ELISA immunoassay, respectively) in 35 samples: 10 normal ovarii, 10 benign, 10 primary malignant and 5 metastatic ovarian tumors. Results. The concentrations of Cath D were significantly higher in malignant (32.89+-14.26 pmol/mg protein ) and metastatic (31.42+-10.24 pmol/mgprotein), than in normal (13.68+-4.03 pmol/mg protein) and benign (17.89+-13.13 pmol/mg protein) ovarian tissues. There was no statistical differences in the concentrations of PAI-1 between normal, benign, malignant and metastatic tumor specimens. The concentrations of Cath D as well as PAI-1 did not correlate to the age of patients, menopausal status, parity, GOG risk group, clinical stage or pathohistological grading. Conclusion. Concentrationsof Cath D (but not PAI-1) were significantly increased in malignant and metastatic ovarian tumor tissues when compared to normal and benign ovarian tumor samples; they were independent from pathohistological andclinical parameters. Published in DiRROS: 23.01.2024; Views: 131; Downloads: 51 Full text (442,49 KB) |
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5. Changes in the quantity of cathepsin D in irradiated human cells following treatment with hyperthermia and interferon [alpha]Danilo Petrović, Ana Ferle-Vidović, Janez Škrk, Marija Kaštelan, Ivan Vrhovec, 1993, original scientific article Keywords: radiacija, biokemija, encimi, proteini, katepsin, celice Published in DiRROS: 10.01.2024; Views: 128; Downloads: 35 Full text (235,25 KB) |
6. Effects of irradiation and THP-Adriamycin on the proteinase activity profiles in cultured V79 cellsDanilo Petrović, Ana Ferle-Vidović, Janez Škrk, Alojz Suhar, Vito Turk, 1993, original scientific article Keywords: radiacija, biokemija, encimi, proteini, fibroblasti, celice Published in DiRROS: 10.01.2024; Views: 160; Downloads: 32 Full text (553,47 KB) |
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10. Modificirajući uticaj na neke endokrine žlezde miša kod kasnih posledica subletalnog jonizacionog zračenjaMiroslav Kališnik, Olga Vraspir-Porenta, Metka Logonder-Mlinšek, Marjeta Zorc, Tatjana Lejko-Zupanc, Alenka Rus-Gaši, Draga Štiblar-Martinčič, Janez Škrk, 1979, original scientific article Published in DiRROS: 14.09.2023; Views: 242; Downloads: 55 Full text (558,37 KB) |