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Evaluation of shRNA-mediated gene silencing by electroporation in LPB fibrosarcoma cells
Suzana Vidic, Urška Kamenšek, Maja Čemažar, 2008, original scientific article

Abstract: Background. Silencing oncogenes or other genes that contribute to tumor malignancy and progression offers a promising approach to treating cancer. Specific and efficient silencing of gene expression can be achieved by RNA interference (RNAi) technology using small interfering RNA (siRNA) or short hairpin RNA (shRNA). However, a major challenge in RNAi technology is effective delivery of interfering molecules into target cells. The aim of our study was to evaluate electroporation as a perspective method for efficient invitro transfection of LPB fibrosarcoma cells with plasmid DNA expressing shRNA. Methods. Induction of shRNA-mediated gene silencing by electroporation was determined by fluorescence microscopy, flow cytometry and western blot analysis. The effect of electroporation conditions on cell survival and proliferation was determined by clonogenic assay. Results and conclusions. Ourresults demonstrated that electroporation is a feasible and effective method for delivery of plasmid DNA expressing shRNA into cancer cells in vitro. Electrotransfection of murine LPB fibrosarcoma cells, continuously expressing green fluorescence protein - GFP (LPBGFP), with plasmid DNA encoding shRNA-GFP, reduced GFP expression, which was determined on the protein level, as well as by measurement of GFP fluorescence intensity. A pronounced reduction in GFP expression level was detected from the second to the fifth day after treatment. Moreover, the method is easy to perform and showed low cell damaging effects, which are the most important and preferential factors for further in vivo studies.
Published in DiRROS: 08.03.2024; Views: 58; Downloads: 20
.pdf Full text (391,23 KB)

15.
What is the most common mammographic appearance of T1a and T1b invasive breast cancer?
Maja Podkrajšek, Janez Žgajnar, Marko Hočevar, 2008, original scientific article

Published in DiRROS: 07.03.2024; Views: 69; Downloads: 19
.pdf Full text (204,48 KB)

16.
Kadar je zdravstveni delavec bolnik ali bolnikov bližnji
Maja Ebert Moltara, 2023, published scientific conference contribution abstract

Keywords: zdravstveni delavci, bolniki, onkologija
Published in DiRROS: 27.02.2024; Views: 84; Downloads: 29
.pdf Full text (238,57 KB)

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Electrochemotherapy of tumours
Gregor Serša, Maja Čemažar, Damijan Miklavčič, Zvonimir Rudolf, 2006, review article

Abstract: Electroehemotherapy consists of chemotherapy followed by local application of electrie pulses to the tumour to increase drug delivery into cells. Drug uptake can be inereased by electroporation for only those drugs whose transport through the plasma membrane is impeded. Among many drugs that have been tested so far, only bleomycin and cisplatin found their way from preclinical testing to clinical trials. In vitro studies demonstrated several fold inerease of their cytotoxicity after electroporation of cells. In vivo, electroporation of tumours after local or systemic administration of either ofthe drugs, i.e. electrochemotherapy, proved to be an effective antitumour treatment. In preclinical studies on several tumour models, electrochemotherapy either with bleomycin or cisplatin was elaborated and parameters for effective local tumour control were determined. In veterinary medicine, electrochemotherapy also proved to be effective in the treatment of primary tumours in cats, dogs and horses. In human clinical studies, electrochemotherapy was performed on the patients with progressive disease andaccessible tumour nodules of different malignancies. All clinical studies demonstrated that electrochemotherapy is an effective treatment for local tumour control in cancer patients.
Published in DiRROS: 15.02.2024; Views: 118; Downloads: 32
.pdf Full text (225,92 KB)

18.
Schedule-dependency of doxorubicin and vinblastine in EAT tumours in mice
Marija Auersperg, Ana Pogačnik, Veronika Kloboves-Prevodnik, Gregor Serša, Maja Čemažar, 2006, original scientific article

Abstract: Background. Antitumour schedule-dependency of the doxorubicin and vinblastine combination was explored. Materials and methods. Intraperitoneal Ehrlich ascites tumours (EAT) syngeneic to CBA mice were treated with vinblastine ar doxorubicin alone, or in combined treatment schedules. Results. Combinations of doxorubicin and vinblasfine administered at 48-h, but not at 24-h interval,regardless of the sequence of drugs, significantly reduced the numberof tumour cells in the ascites in corrtparison with all other treatments. In the combined treatment schedules, the predominant morphologicalchanges as well as DNA distribution pattern were dependent on thefirst drug applied. Regardless of the sequence of the drugs, median survival times of animals did not significantly differ between the treatment groups. Conclusions. The effect of combination of vinblastine and doxorubicin is schedule-dependent. The time interval, but not the sequence of drugs seems to be crucial for the observed effect. The data from preclinical studies are important for planning combined treatment schedules in clinical setting.
Published in DiRROS: 15.02.2024; Views: 78; Downloads: 15
.pdf Full text (250,21 KB)

19.
Complete yearly life tables by sex for Slovenia, 1982-2004, and their use in public health
Tina Žagar, Vesna Zadnik, Maja Pohar Perme, Maja Primic-Žakelj, 2006, original scientific article

Published in DiRROS: 15.02.2024; Views: 78; Downloads: 18
.pdf Full text (1,84 MB)

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Bibliometrična analiza objav Gozdarskega instituta Slovenije za obdobje 2011-2020
Maja Peteh, 2020, treatise, preliminary study, study

Keywords: bibliometrija, Gozdarski inštitut Slovenije, raziskovalna dejavnost
Published in DiRROS: 12.02.2024; Views: 132; Downloads: 36
.pdf Full text (419,92 KB)

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