1. Nontargeted urinary profiling strategy for endocrine-disrupting chemicals in women with ovarian malignanciesHelena Plešnik, Žan Rekar, Stefanela Stevanović, Irma Virant-Klun, Senka Imamović-Kumalić, Mateja Sladič, Darja Mazej, Janja Snoj Tratnik, Milena Horvat, Tina Kosjek, 2025, izvirni znanstveni članek Povzetek: Endocrine-disrupting chemicals (EDCs), includingknown and unknown parent compounds, their metabolites, andtransformation products, are pervasive in daily life, posingincreasing risks to human health and the environment. Thisstudy employed a high-resolution mass spectrometry-basednontargeted screening approach, integrating polar (HILIC) andreversed-phase separations to expand the chemical space coverageand, supported by open-science tools and resources, evaluatedurinary chemical profiles to assess internal EDC exposure. Among106 annotated biomarkers of exposure, six exhibited significantlyhigher normalized intensities in patients with ovarian malignanciescompared to healthy controls (p < 0.05). This suggests theirgreater exposure to phthalates (diethylhexyl phthalate and diethylphthalate), pesticides (metolachlor metabolite and 4-nitrophenol), a UV filter (benzophenone-1), and an industrial byproduct (4-methyl-2-nitrophenol). These compounds may interfere with hormonal regulation, potentially contributing to cancer development.While these findings highlight potential differences in internal EDC exposure, the study primarily demonstrates the applicability ofnontargeted urinary profiling for chemical exposure assessment. By providing new insights into EDCs burden and its pathologicalimplications, this work contributes to advancing next-generation chemical risk assessment within the European Partnership for theAssessment of Risks from Chemicals initiative and supports the development of preventive strategies to mitigate environmentalcancer risks
Ključne besede: ovarian cancer, biomarkers, exposure, nontargeted screening, high-resolution mass spectrometry, analytical coverage, reversed-phase, HILIC
Objavljeno v DiRROS: 02.12.2025; Ogledov: 410; Prenosov: 139
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2. Influence of natural variability and anatomical misalignment on the correlation between segmental myocardial edema and strain in acute myocarditisKanza Awais, Lana Kralj, Andreja Černe Čerček, Borut Kirn, 2025, izvirni znanstveni članek Povzetek: Background: Acute myocarditis (AM) affects myocardial structure and function, assessed by cardiac magnetic resonance late gadolinium enhancement (CMR-LGE) and speckle tracking echocardiography (STE), respectively; however, the correlation between the two techniques at the segmental level is inconsistent. We studied natural heterogeneity and anatomical orientation mismatch as potential causes of correlation discrepancy. Methods: A total of 30 AM patients underwent left ventricle LGE-CMR and STE measurement, acquiring 18 segmental values depicting edema extent and peak longitudinal strain, respectively. Baseline segmental correlation was compared to average patient segmental correlation and to segmental correlation after spatial resolution reduction achieved by averaging adjacent segments in four successive iterations, where the degree of spatial resolution reduction was evaluated based on the relative decrease in segmental standard deviation. Results: Baseline segmental correlation was weak, i.e., r = 0.24 (p < 0.05) but improved in fitted SLGE and SpLS baseline correlation (r0 = 0.44, p < 0.05) and in average patient correlation (r = 0.55, p < 0.05). Iterative spatial resolution reduction increased the correlation to r1 = 0.49 and r2 = 0.51 and then decreased it to r3 = 0.11 (p < 0.05) and r4 = 0.07 (p > 0.05), with corresponding decreases in segmental standard deviation relative to baseline from σ0 = 12.87 to σ/σ0 = 0.68, 0.51, 0.38, and 0.29 in SLGE values and σ0 = 4.77 to σ/σ0 = 0.57, 0.41, 0.31, and 0.23 in SpLS. Conclusions: Improved correlation in average patients is associated with natural heterogeneity, which indicates a need to develop more robust indicators of ventricular function. The improved correlation in moderate spatial resolution reduction indicates a potential solution for anatomic orientation mismatch between CMR-LGE and STE techniques.
Ključne besede: acute myocarditis, anatomical orientation mismatch, cardiac magnetic resonance, late gadolinium enhancement, natural heterogeneity, segmental peak systolic strain, spatial resolution reduction, speckle tracking echocardiography
Objavljeno v DiRROS: 10.11.2025; Ogledov: 272; Prenosov: 126
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3. A cross-sectional study of laboratory parameters 5–6 months after the first COVID-19 infectionTaja Zore, Jasna Lojk, Katarina Reberšek, Elizabeta Božnar Alič, Urška Čegovnik Primožič, Alenka France Štiglic, Aleš Jerin, Irena Prodan Žitnik, Helena Podgornik, Nada Snoj, Barbara Ostanek, Gabriele Turel, Tatjana Lejko-Zupanc, Janja Marc, Darko Černe, 2025, izvirni znanstveni članek Povzetek: Objectives: Despite extensive study of COVID-19 disease, only a few studies also addressed the aftermath of the disease and potential long-term consequences. The aim of this study was to assess COVID-19 resolution through the cross-sectional analysis of an extensive range of haematological and biochemical laboratory parameters and to find potential markers still associated with disease severity 5-6-months post infection.
Methods: In this study, we analysed 92 routine biochemical, haematological and immunological parameters in 75 non-vaccinated patients 5–6 months after recorded first time SARS-CoV-2 infection without reinfection. Demographic and disease severity data were obtained through surveys.
Results: The majority of analysed parameters were within the normal reference intervals, however, statistically significant correlations with the disease severity were detected in 15 parameters: B lymphocytes, NK cells, interleukin (IL)-12, IL-1β, cortisol, ferritin, SARS-CoV-2 specific IgG and IgM antibodies, Na, Cl, creatinine, alkaline phosphatase, cholesterol, HbA1c and alpha 2 and beta 2 globulin fractions of the proteinogram.
Conclusions: Although most observed parameters returned to their normal reference intervals, significant correlations were still observed with disease severity, that could indicate either the pre-infection baseline state which affected disease outcome or minor remaining alterations in function of certain organs, pertaining their stress or damage during the acute phase of the disease.
Ključne besede: disease severity, laboratory parameters, resolution, COVID-19, SARS-CoV-2, laboratory diagnosis
Objavljeno v DiRROS: 07.11.2025; Ogledov: 314; Prenosov: 135
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4. Partial asynchrony of coniferous forest carbon sources and sinks at the intra-annual time scaleRoberto Silvestro, Maurizio Mencuccini, Franco Biondi, Jesus Julio Camarero, Alberto Arzac, Filipe Campelo, Katarina Čufar, Henri E. Cuny, Martin De Luis, Annie Deslauriers, Jožica Gričar, Peter Prislan, 2024, izvirni znanstveni članek Povzetek: As major terrestrial carbon sinks, forests play an important role in mitigating climate change. The relationship between the seasonal uptake of carbon and its allocation to woody biomass remains poorly understood, leaving a significant gap in our capacity to predict carbon sequestration by forests. Here, we compare the intra-annual dynamics of carbon fluxes and wood formation across the Northern hemisphere, from carbon assimilation and the formation of non-structural carbon compounds to their incorporation in woody tissues. We show temporally coupled seasonal peaks of carbon assimilation (GPP) and wood cell differentiation, while the two processes are substantially decoupled during off-peak periods. Peaks of cambial activity occur substantially earlier compared to GPP, suggesting the buffer role of non-structural carbohydrates between the processes of carbon assimilation and allocation to wood. Our findings suggest that high-resolution seasonal data of ecosystem carbon fluxes, wood formation and the associated physiological processes may reduce uncertainties in carbon source-sink relationships at different spatial scales, from stand to ecosystem levels.
Ključne besede: carbon sinks, forests, conifers, intra-annual resolution
Objavljeno v DiRROS: 10.02.2025; Ogledov: 656; Prenosov: 494
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