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Povzetek: Endoscope reprocessing is a time-consuming, multi-step process, and ongoing microbial surveillance is necessary to ensure effective reprocessing and safe storage. Despite this, traditional surveillance methods, such as cultures, are not often carried out due to time and cost constraints, providing only delayed end point results from specific locations, with no insight into biofilm formation, disinfection efficacy, or any variability related to device design. Within this study, the efficacy of plasma activated water (PAW) disinfection (and subsequent regrowth) within endoscopic test pieces was investigated using bacterial bioluminescence enabling real time and in situ monitoring of biofilm formation and treatment efficacy. Real time imaging of bioluminescent Pseudomonas aeruginosa PAO1 SEI MCS5-lite was used to track biofilm growth within translucent PVC endoscopic test pieces across regions of interest using analysis of bioluminescent intensity. Results demonstrated that biofilm accumulation was more prominent around connectors compared to other regions of the test pieces. Disinfection with PAW achieved a significant 96.45% reduction in biofilm density (determined by culture) and a 93.08% reduction in bioluminescence (relative light units). However, percentage reduction in bioluminescence ranged from 77% to 95% across regions indicating lack of uniformity of treatment. Subsequent testing with plasma activated disinfectant (PAD) showed consistent biofilm reduction across all regions, with no variability between connectors and other regions as observed with PAW and other treatments. In conclusion, bioluminescence can be used to assess efficacy of disinfectants and effect of endoscope design by tracking biofilm density in real time and in situ.
Ključne besede: cold atmospheric plasma, plasma activated water, endoscope reprocessing
Objavljeno v DiRROS: 06.02.2026; Ogledov: 130; Prenosov: 89
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Povzetek: Idiopathic nephrotic syndrome (INS) is the most prevalent glomerular illness in children. Even while immunologic processes are well-established, oxidative stress is becoming more widely acknowledged as a significant factor in the etiopathogenesis of illness. Assessing its activity and treatment response may be made easier with the use of trustworthy, non-invasive indicators to track redox balance. We report on the oxidative stress levels of a 10.7-year-old boy with INS with five clinical time points in one year. The FRAS5 analyzer was used to calculate the oxidative stress index (OSI), plasma antioxidant capacity (PAT) and derivatives of reactive oxygen metabolites (d-ROMs) as biomarkers. A 4-tier oxidative state classification scheme based on d-ROM and PAT thresholds was used to interpret the values. The patient had low antioxidant defense, moderate oxidative and increased OSI at relapses, a positive transition to reduced oxidative burden and enhanced defense during remission. The order of events showed a dynamic redox response associated with glucocorticoid (GC) medication and disease activity. The potential value of d-ROM, PAT, and OSI as dynamic biomarkers for tracking disease activity, response to treatment and residual oxidative burden in pediatric INS is supported by this case. To confirm their function in more comprehensive clinical decision-making, more research is required.
Ključne besede: idiopathic nephrotic syndrome, children, derivatives of reactive oxygen metabolites, plasma antioxidant capacity, oxidative stress index
Objavljeno v DiRROS: 06.01.2026; Ogledov: 190; Prenosov: 95
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Povzetek: Introduction: Accurate measurement of rosuvastatin in plasma is critical for effective patient management and treatment monitoring following myocardial infarction (MI). Expensive solid-phase extraction (SPE) and timeconsuming liquid-liquid extraction (LLE) have been established for quantifying rosuvastatin. Supported liquid extraction (SLE) could offer a rapid, cost-effective alternative. Objectives: This study aimed to develop and validate a rapid, cost-effective, accurate, and precise method for quantifying rosuvastatin in high-dose plasma samples from patients following MI. Methods: Rosuvastatin was extracted from EDTA plasma using SLE and quantified with LC-MS/MS with positive electrospray ionization. The method was validated according to ICH M10 guidelines, focusing on selectivity, matrix effect, accuracy, precision, linearity, and carryover. Rosuvastatin-D6 was used as an internal standard. Additionally, thirty plasma samples from patients on high-dose rosuvastatin therapy (20 or 40 mg/day) following MI were analyzed by both LLE and SLE methods and compared. Results: The method was successfully validated, demonstrating linearity across a range of 0.1 ng/mL to 50 ng/mL. Compared to the LLE method, SLE achieved superior extraction recovery (96.3 % vs. 60 %) and precision (RSD: 11.9 % vs. 13.6 %) at 0.3 ng/mL rosuvastatin, with a lower absolute matrix effect (12.7 % vs.-36.7 %). Accuracy was comparable (109.3 % vs. 92.8 %). Although SLE involves higher initial costs, it significantly enhances throughput, reduces solvent usage, and minimizes contamination and equipment wear. Conclusion: This study validates SLE as a superior method for quantifying rosuvastatin in plasma, outperforming LLE in recovery, reproducibility, and automation. SLE offers greater accuracy and reliability, making it ideal for high-throughput applications.
Ključne besede: supported liquid extraction, liquid-liquid extraction, rosuvastatin, LC-MS/MS, myocardial infarction, solid-phase extraction, blood plasma
Objavljeno v DiRROS: 24.11.2025; Ogledov: 827; Prenosov: 138
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Ključne besede: polytetrafluoroethylene, fluorine depletion, hydrogen plasma, VUV radiation, surface modification, hydrophilic
Objavljeno v DiRROS: 10.11.2025; Ogledov: 286; Prenosov: 130
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Povzetek: The phase composition and microstructure of Sr-hexaferrite (SFO) ceramics govern the hard magnetic properties. Here, pressureless spark plasma sintering (pSPS) technique was employed for the rapid consolidation (500°C/min) of SFO in a radiating graphite crucible under vacuum and, thus, reductive conditions. A numerical model depicting the temperature profile within the heating crucible was constructed to understand the temperature evolution within the samples. The combination pSPS sintering environment (graphite heating crucible under vacuum) promoted phase decomposition to Sr-enriched and depleted phases of various morphological variations, leading to reduction of hard magnetic properties. Notably, certain newly formed phases exhibited lower melting points, inducing a shift in the sintering mechanism from solid-state sintering to a partial liquid-phase sintering mechanism.
Ključne besede: pressureless spark plasma sintering, electronic ceramics, numerical simulations
Objavljeno v DiRROS: 03.09.2025; Ogledov: 458; Prenosov: 195
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