Iskanje po repozitoriju

A+ | A- | | SLO | ENG

Povzetek: Plant genetic engineering represents an important aspect of modern agriculture, and new geneticallymodified (GM) crop varieties are entering the market on a regular basis. This necessitates thedevelopment of high throughput multi-target analytical methods to detect and quantify theirpresence for regulatory compliance. In this study, we present a multiplex dPCR method for discrimi-native quantification of 19 GM soybean events and the lectin (Le1) endogene on a nanowell plate-based all-in-one dPCR system. The method consists of four 5-plex assays, taking advantage of theplatform’s multiple fluorescence detection channels. The assays complied with the minimum perfor-mance requirements in terms of specificity, trueness, precision, sensitivity and dynamic range,making them suitable for use in routine detection and quantification of GM crops. This methodrepresents the most comprehensive multi-target GM soybean quantification approach to date with-out the need for prior screening and features a simplified workflow, making it suitable for widespreadadoption. Our study sets a precedent for rapid and straightforward development of multiplex dPCRGM crop quantification assays to address the evolving demands of regulatory monitoring.
Ključne besede: genetically modified (GM) crops, transgenic, soybean, Glycine max (L.) merr, quantification, digital PCR (dPCR), multiplex, multi-target
Objavljeno v DiRROS: 19.03.2026; Ogledov: 173; Prenosov: 127
Celotno besedilo (1,03 MB)
Gradivo ima več datotek! Več...

Povzetek: This study explores the application of strontium (Sr) isotope ratios (87Sr/86Sr) as geochemical tracers to understand hydrological connectivity and anthropogenic influences in a geologically diverse Alpine river catchment encompassing regions of Austria and Slovenia. Focusing on the Mur River and its tributaries, water samples were collected across 45 sites during three hydrological regimes to capture seasonal and spatial variability. In total, 28 tributaries were sampled to evaluate their influence on the isotope composition of the main river. Isotope analyses using multi-collector inductively coupled plasma mass spectrometry, supplemented by geospatial mapping and multivariate statistics, were combined with measurements of 26 elemental concentrations to investigate land–water interactions and human impacts. The results demonstrate substantial temporal and spatial variability in 87Sr/86Sr ratios (ranging from 0.70853 to 0.71322), reflecting both geological heterogeneity and varying tributary inputs. A preliminary aquatic isoscape was developed, enabling the monitoring of connectivity and the impacts of geological and human influences under varying flow regimes. Tributaries, such as the Mürz River, significantly modulate isotope signatures in the main channel, often overriding local geological signals. The application of isotope pattern deconvolution revealed a contribution of 17% from the Mürz River to the Mur River’s isotope signature downstream of the confluence. Correlations between 87Sr/86Sr and metal concentrations further suggest the utility of 87Sr/86Sr as a proxy for source identification and anthropogenic influence assessment. By constructing a preliminary strontium isoscape for the region, this research provides novel insights into riverine connectivity, catchment-scale processes, and ecosystem dynamics. The integration of isotope data contributes to interdisciplinary understanding across geological, chemical, and ecological boundaries, and supports the development of isotope-based tools for sustainable aquatic system monitoring and management, including potential applications in fish ecology and habitat tracking.
Ključne besede: environmental monitoring, Sr isotopes, multi-element analysis, river management, river catchment tracing
Objavljeno v DiRROS: 13.02.2026; Ogledov: 456; Prenosov: 115
Celotno besedilo (2,61 MB)

Povzetek: To ensure appreciation of forest related nature‘s services, they must be presented to the public in an appropriate way. Humans are usually not willing to pay for something they consider as ‘free goods’. The challenge is changing the mind-set through awareness-raising. A good communication strategy should convey the message as a tangible concern that contributes to the quality of our lives. Communicating nature’s services will help understand the benefits we gain and joy and pleasure they can give us if we treat them with knowledge, respect and wisdom. The actions taken in the past are not giving the results we need. A multi-stakeholder approach can harness the creativity from stakeholders through solution driven dialogue. In the LIFEGENMON project (Life for Forest Genetic Monitoring System) coordinated by Slovenian Forestry Institute, the multi-stakeholder approach is used to communicate the forest related nature’s services and importance of genetic resources. A clear visual and verbal communication brings the key project messages to life. To forge meaningful connections with core stakeholders it is essential to use compelling and targeted messages. Ultimately, the results will demonstrate the value of engaging supporters in co-creating communications to extend reach and impact.
Ključne besede: nature’s services, communicating, forests, society, forest values, multi-stakeholder approach
Objavljeno v DiRROS: 28.01.2026; Ogledov: 247; Prenosov: 182
Celotno besedilo (147,12 KB)
Gradivo ima več datotek! Več...

Povzetek: We experimentally assessed the capabilities of two mainstream artificial intelligence chatbots, ChatGPT and DeepSeek, to support the multi-criteria decision-making process. Specifically, we focused on using the method DEX (Decision EXpert) and investigated their performance in all stages of DEX model development and utilization. The results indicate that these tools may substantially contribute in the difficult stages of collecting and structuring decision criteria, and collecting data about decision alternatives. However, at the current stage of development, the support for the whole multi-criteria decision-making process is still lacking, mainly due to occasionally inconsistent and erroneous execution of methodological steps. To leverage the strengths of both approaches, we also propose a hybrid workflow for DEX model development that begins in the LLM and continues in the specialized DEXiWin software.
Ključne besede: multi-criteria decision making, DEX method, decision analysis, large language models
Objavljeno v DiRROS: 28.01.2026; Ogledov: 325; Prenosov: 177
Celotno besedilo (601,46 KB)
Gradivo ima več datotek! Več...

Povzetek: This study presents a multi-analytical investigation of the wooden components from Khufu’s Second Solar Boat, one of the valuable archaeological discoveries in ancient Egypt. The research integrates advanced imaging and analytical techniques to identify wood species, assess deterioration patterns, and characterize the fungal and bacterial biodeteriogens. The initial visual examination documented the state of preservation at the time of discovery. Subsequently, the identification of the wood species was carried out. The deterioration status was assessed using a variety of tools, including scanning electron microscopy (SEM) and high-resolution synchrotron radiation computed microtomography (SR-μCT) for morphological alterations, X-ray diffraction (XRD) for crystallinity changes and Fourier-transform infrared spectroscopy (FTIR) to assess chemical degradation indexes of wood. Moreover, molecular techniques were used to identify and characterize the presence of biodeterioration agents. Results indicate that ancient craftsmen used cedar wood for the boat construction. The analysed samples exhibited advanced biotic and abiotic degradation, as evidenced by microbiological assessments, XRD measurements of cellulose crystallinity, FTIR indices, SEM micrographs and SR-μCT data. These results provide crucial insights into the long-term degradation processes of archaeological wood in arid environments, enhancing our understanding of ancient Egyptian woodworking practices and informing future conservation strategies for similar artifacts.
Ključne besede: archaeological wood conservation, multi-analytical characterization, biodeterioration analysis, ancient egyptian woodworking, x-ray computed microtomography, lebanon cedar
Objavljeno v DiRROS: 13.01.2026; Ogledov: 272; Prenosov: 243
Celotno besedilo (4,27 MB)
Gradivo ima več datotek! Več...