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Ključne besede: cast aluminium alloys, sol-gel coatings, field testing, scanning electron microscopy, x-ray photoelectron spectroscopy, marine immersion, Adriatic Sea
Objavljeno v DiRROS: 25.04.2025; Ogledov: 155; Prenosov: 59
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Ključne besede: marine strategy, indicators, reference situations, integration rules
Objavljeno v DiRROS: 23.04.2025; Ogledov: 167; Prenosov: 0
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Ključne besede: marine strategy, eutrophication, good environmental status, methodology
Objavljeno v DiRROS: 23.04.2025; Ogledov: 164; Prenosov: 0
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Povzetek: Molecular methods such as DNA/eDNA metabarcoding have emerged as useful tools to document the biodiversity of complex communities over large spatio-temporal scales. We established an international Marine Biodiversity Observation Network (ARMS-MBON) combining standardised sampling using autonomous reef monitoring structures (ARMS) with metabarcoding for genetic monitoring of marine hard-bottom benthic communities. Here, we present the data of our first sampling campaign comprising 56 ARMS units deployed in 2018–2019 and retrieved in 2018–2020 across 15 observatories along the coasts of Europe and adjacent regions. We describe the open-access data set (image, genetic and metadata) and explore the genetic data to show its potential for marine biodiversity monitoring and ecological research. Our analysis shows that ARMS recovered more than 60 eukaryotic phyla capturing diversity of up to ~5500 amplicon sequence variants and ~1800 operational taxonomic units, and up to ~250 and ~50 species per observatory using the cytochrome c oxidase subunit I (COI) and 18S rRNA marker genes, respectively. Further, ARMS detected threatened, vulnerable and non-indigenous species often targeted in biological monitoring. We show that while deployment duration does not drive diversity estimates, sampling effort and sequencing depth across observatories do. We recommend that ARMS should be deployed for at least 3–6 months during the main growth season to use resources as efficiently as possible and that post-sequencing curation is applied to enable statistical comparison of spatio-temporal entities. We suggest that ARMS should be used in biological monitoring programs and long-term ecological research and encourage the adoption of our ARMS-MBON protocols.
Ključne besede: 18S rRNA, COI, essential biodiversity variables, European marine omics biodiversity observation network, genetic monitoring, invasive species, ITS
Objavljeno v DiRROS: 18.04.2025; Ogledov: 177; Prenosov: 71
Celotno besedilo (3,67 MB)
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Povzetek: Canopy-forming brown macroalgae (Fucales) offer numerous key ecosystem services in Mediterranean coastal areas. However, anthropogenic pressures and climate change have significantly impacted their habitats, leading to an extensive population decline. Interactions between algae and microbiota are a major ecological aspect, yet they represent a significant knowledge gap. In our baseline study, we describe the diversity and host specificity of the microbiome of two genetically identical but morphologically distinct populations of Gongolaria barbata from anthropogenically impacted northern Adriatic Sea. Our preliminary results showed that the microbiomes of G. barbata exhibited low host specificity, with 75% of the algae-associated amplicon sequence variants (ASVs) being part of the core coastal ecosystem microbiome. However, microbiomes of specific algal parts, ambient seawater, and sediment differed significantly in terms of alpha diversity and composition. In contrast, the holdfast and axis show higher similarity with sediment microbiomes, indicating potential horizontal transmission pathways. Microbiomes associated with deciduous parts of morphologically distinct G. barbata populations showed no difference in alpha diversity and composition. In contrast, higher variation in alpha diversity and lower sequence proportion of shared ASVs were observed in the holdfast and axis of the two distinct populations. Our observational study provides valuable new insights and baseline for future hypothesis-driven research on the interactions between algae and associated microbiota—a knowledge gap that needs to be addressed in the future for better understanding of the ecological and evolutionary dynamics of coastal ecosystems.
Ključne besede: ecosystem services, anthropogenic pressures, microbiome, host specificity, alpha diversity, marine biology, ecology
Objavljeno v DiRROS: 18.04.2025; Ogledov: 153; Prenosov: 121
Celotno besedilo (1,35 MB)
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