1. Potential of soluble immune checkpoints as prognostic and predictive biomarkers in endometrial cancerBoštjan Pirš, Maja Pušić Novak, Luka Roškar, Tea Lanišnik-Rižner, Špela Smrkolj, 2025, objavljeni znanstveni prispevek na konferenci Ključne besede: endometrial cancer, mismatch repair deficiency, biomarkers, liquid biopsy
Objavljeno v DiRROS: 18.03.2026; Ogledov: 183; Prenosov: 79
 Celotno besedilo (348,04 KB) |
2. Somatic mutation detection in tumor tissue and matched cell-free DNA using PCR-based methods in pancreatic cancer patients undergoing upfront resectionHana Zavrtanik, David Badovinac, Tanja Blagus, Katja Goričar, Branislava Ranković, Alenka Matjašič, Andrej Zupan, Aleš Tomažič, Vita Dolžan, 2025, izvirni znanstveni članek Povzetek: Somatic mutations in KRAS and TP53 are among the most common genetic alterations in pancreatic ductal adenocarcinoma (PDAC). Advances in PCR-based technologies now enable the detection of these mutations in tumor tissue and cell-free DNA (cfDNA), providing a minimally invasive approach to assess tumor burden. However, in resectable PDAC, circulating tumor DNA (ctDNA) may represent less than 0.1% of total cfDNA, requiring highly sensitive detection methods. The aim of our study was to assess two PCR-based assays—competitive allele-specific PCR (castPCR) and digital PCR (dPCR)—for detecting selected somatic mutations in tumor tissue, cfDNA, and extracellular vesicle-associated DNA (EV-DNA) from plasma. Matched primary tumor and preoperative plasma samples were collected from 50 patients undergoing upfront resection for PDAC. CastPCR was used for detecting selected KRAS, TP53, SMAD4, and CDKN2A mutations in tumor DNA. Additionally, dPCR was used to analyze KRAS and TP53 mutations in tumor DNA as well as cfDNA and EV-DNA. The concordance between both platforms was 71.4% for KRAS p.G12D and 58.3% for the analysis of TP53 p.R273H mutations in tumor tissue. However, dPCR detected these mutations in an additional 28.6% and 39.6% of samples, respectively. In cfDNA, dPCR identified KRAS p.G12D in 10.2% and TP53 p.R273H in 2.0% of samples. Mutation detection in EV-DNA was limited by low DNA yield. Both platforms proved effective for tumor DNA analysis, with dPCR offering greater sensitivity. Somatic mutation detection from liquid biopsy using dPCR further supports its potential utility in the preoperative setting.
Ključne besede: somatic mutation detection, cell-free DNA, liquid biopsy, digital PCR, pancreatic cancer
Objavljeno v DiRROS: 08.01.2026; Ogledov: 437; Prenosov: 184
Celotno besedilo (1,19 MB)
Gradivo ima več datotek! Več... |
3. Assessment of extracellular particles directly in diluted plasma and blood by interferometric light microscopy : a study of 613 human and 163 canine samplesBoštjan Korenjak, Armando Tratenšek, Matevž Arko, Anna Romolo, Matej Hočevar, Matic Kisovec, Maxence Berry, Apolonija Bedina Zavec, David Drobne, Tomaž Vovk, Aleš Iglič, Alenka Nemec Svete, Vladimira Erjavec, Veronika Kralj-Iglič, 2024, izvirni znanstveni članek Ključne besede: extracellular particles, extracellular vesicles, exosomes, membrane vesiculation, platelets, blood products, platelet-rich plasma, liquid biopsy, membrane enclosed, drug delivery
Objavljeno v DiRROS: 20.12.2024; Ogledov: 1026; Prenosov: 640
Celotno besedilo (1,96 MB)
Gradivo ima več datotek! Več... |
4. |
