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Iskalni niz: "ključne besede" (human biomonitoring) .

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1.
Optimisation of a sample preparation method for the determination of multi-elemental compositions in human hair by triple quadrupole ICP-MS analysis
Agneta Annika Runkel, Marta Jagodic Hudobivnik, Igor Živković, Polona Klemenčič, Darja Mazej, Milena Horvat, 2026, izvirni znanstveni članek

Povzetek: Monitoring toxic elements has a long tradition in Slovenia due to historical mining. More recently, attention has shifted to essential elements, since both deficiencies and excesses can harm health. Regular monitoring of (non-)essential elements supports risk assessment and policymaking. While urine and blood are common biomonitoring matrices, hair offers a non-invasive alternative that reflects exposure over several months, though standardised methodologies for hair analysis remain limited. This study aimed to develop and validate a sensitive and robust analytical method for the determination of 29 elements in human hair, addressing key challenges in sample preparation and contamination control. We developed a sensitive and robust method for the determination of 29 elements (Ag, Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, Ni, P, Pb, Rb, S, Sb, Se, Sn, Sr, Ti, U, V, and Zn) in 3 cm segments of human hair that involves a washing procedure with acetone and Milli-Q water, microwave digestion with 65% HNO3, and analysis with Triple Quadrupole Inductively Coupled Plasma Mass Spectrometry (ICP-MS/MS). Evaluation of preparation steps revealed stainless-steel scissors as a major contamination source. Glass digestion vessels were unsuitable for several elements due to high detection limits and relative standard deviations. The optimised method reduced analytical variability and improved sensitivity compared to published protocols. This validated method enables reproducible multi-elemental analysis in hair, highlights overlooked contamination risks, and is now applied in human biomonitoring studies to strengthen exposure assessment and standardisation efforts.
Ključne besede: determination of elements, optimization, human biomonitoring
Objavljeno v DiRROS: 27.01.2026; Ogledov: 182; Prenosov: 105
.pdf Celotno besedilo (1,59 MB)
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2.
Quantification of phthalate and DINCH metabolites in human urine and maternal breast milk : assessing maternal body burden and infant exposure
Agneta Annika Runkel, Žan Rekar, Neja Kosirnik, Darja Mazej, Milena Horvat, Janja Snoj Tratnik, Tina Kosjek, 2026, izvirni znanstveni članek

Povzetek: Based on toxicological evidence, human exposure to phthalates (PHs) and diisononylcyclohexane-1,2-dicarboxylate (DINCH) may contribute to adverse health effects, especially during vulnerable developmental stages. To support the exposure assessment for this group of endocrine disruptors, we developed and validated a method for the analysis of 14 PH and 3 DINCH metabolites in human urine and maternal milk, applied the method in a pilot study, and identified crucial obstacles in the path of establishing maternal milk as a routine matrix in human biomonitoring. Urine and milk samples were extracted with solid-phase extraction (SPE) and QuEChERS salts, respectively, and analysed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). The method accuracy was confirmed for urine samples via a certified standard reference material and the G-EQUAS intercomparison programme. We identified a need for sampling protocols, reference materials, and external method verification schemes in order to establish maternal milk as a routine matrix. Finally, the method was tested for its applicability in a pilot biomonitoring study on 30 paired urine and milk samples from lactating mothers, with medians ranging from Ključne besede: phthalate metabolites, DINCH, human biomonitoring
Objavljeno v DiRROS: 14.01.2026; Ogledov: 180; Prenosov: 113
.pdf Celotno besedilo (2,06 MB)
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