1. European society of clinical microbiology and infectious diseases guidelines for antimicrobial stewardship in emergency departments (endorsed by European association of hospital pharmacists)Teske Schoffelen, Cihan Papan, Elena Carrara, Khalid Eljaaly, Paul Mical, Emma Keuleyan, Alejandro Martin Quirós, Nathan Peiffer-Smadja, Bojana Beović, 2024, pregledni znanstveni članek Povzetek: Scope: This European Society of Clinical Microbiology and Infectious Diseases guideline provides evidence-based recommendations to support a selection of appropriate antibiotic use practices for patients seen in the emergency department (ED) and guidance for their implementation. The topics addressed in this guideline are (a) Do biomarkers or rapid pathogen tests improve antibiotic prescribing and/or clinical outcomes? (b) Does taking blood cultures in common infectious syndromes improve antibiotic prescribing and/or clinical outcomes? (c) Does watchful waiting without antibacterial therapy or with delayed antibiotic prescribing reduce antibiotic prescribing without worsening clinical outcomes in patients with specific infectious syndromes? (d) Do structured culture follow-up programs in patients discharged from the ED with cultures pending improve antibiotic prescribing? Methods: An expert panel was convened by European Society of Clinical Microbiology and Infectious Diseases and the guideline chair. The panel selected in consensus the four most relevant antimicrobial stewardship topics according to pre-defined relevance criteria. For each main question for the four topics, a systematic review was performed, including randomized controlled trials and observational studies. Both clinical outcomes and stewardship process outcomes related to antibiotic use were deemed relevant. The literature searches were conducted between May 2021 and March 2022. In April 2022, the panel members were formally asked to suggest additional studies that were not identified in the initial searches. Data were summarized in a meta-analysis if possible or otherwise summarized narratively. The certainty of the evidence was classified according to the Grading of Recommendations Assessment, Development and Evaluation criteria. The guideline panel reviewed the evidence per topic critically appraising the evidence and formulated recommendations through a consensus-based process. The strength of the recommendations was classified as strong or weak. To substantiate the implementation process, implementation trials or observational studies describing facilitators/barriers for implementation were identified from the same searches and were summarized narratively. Recommendations: The recommendations on the use of biomarkers and rapid pathogen diagnostic tests focus on the initiation of antibiotics in patients admitted through the ED. Their effect on the discontinuation or de-escalation of antibiotics during hospital stay was not reported, neither was their effect on hospital infection prevention and control practices. The recommendations on watchful waiting (i.e. withholding antibiotics with some form of follow-up) focus on specific infectious syndromes for which the primary care literature was also included. The recommendations on blood cultures focus on the indication in three common infectious syndromes in the ED explicitly excluding patients with sepsis or septic shock. Most recommendations are based on very low and low certainty of evidence, leading to weak recommendations or, when no evidence was available, to best practice statements. Implementation of these recommendations needs to be adapted to the specific settings and circumstances of the ED. The scarcity of high-quality studies in the area of antimicrobial stewardship in the ED highlights the need for future research in this field.
Ključne besede: antimicrobial stewardship, blood cultures, culture follow-up, diagnostic tests, emergency department, guideline, implementation, rapid tests, recommendations, watchful waiting
Objavljeno v DiRROS: 09.03.2026; Ogledov: 58; Prenosov: 37
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2. Whose Heritage? Examples of Contested and Shared Cultures of Remembrance and Heritage Questions in the Multicultural Formerly Habsburg Cities of Rijeka and MariborAngela Ilić, 2025, izvirni znanstveni članek Povzetek: To whom do material and immaterial heritage belong? Can remembrance cultures be inclusive instead of cancelling each other out? This article explores possible answers to these questions in two historically multicultural cities, Rijeka/Fiume/Reka and Maribor/Marburg, in relation to their heritage of the Late Habsburg period. Drawing on original sources and recent literature in various languages, public attempts at narrating the Habsburg past through exhibitions, monuments and the like are examined.
Ključne besede: remembrance cultures, contested heritage, shared heritage, Rijeka, Maribor
Objavljeno v DiRROS: 07.02.2026; Ogledov: 397; Prenosov: 88
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3. Exploring marine microbial diversity : an overview of representative sampling strategiesZrinka Ljubešić, Marija Gligora Udovič, Donata Overlingė, Filip Grgurević, Füsun Akgül, Ariola Bacu, Ana R. Díaz Marrero, Dragana Drakulović, Stefano Fazi, Susana P. Gaudêncio, Anamarija Kolda, Lucie Novoveská, Ivo Safarik, Joana Sousa, Olivier Thomas, Maggie M. Reddy, Giovanna Cristina Varese, Marlen Ines Vasquez, Tihomir Makovec, Ana Rotter, 2025, pregledni znanstveni članek Ključne besede: biodiscovery, marine microbial diversity, sampling protocol, sample preservation and storage, biorepositories, cultures, marine biology
Objavljeno v DiRROS: 17.10.2025; Ogledov: 473; Prenosov: 213
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4. Expansive growth of two glioblastoma stem-like cell lines is mediated by bFGF and not by EGFNeža Podergajs, Narve Brekka, Bernhard Radlwimmer, Christel Herold-Mende, Krishna M. Talasila, Katja Tiemann, Uroš Rajčević, Tamara Lah Turnšek, Rolf Bjerkvig, Hrvoje Miletic, 2013, objavljeni znanstveni prispevek na konferenci Povzetek: Background. Patient-derived glioblastoma (GBM) stem-like cells (GSCs) represent a valuable model for basic and therapeutic research. GSCs are usually propagated in serum-free Neural Basal medium supplemented with bFGF and EGF. Yet, the exact influence of these growth factors on GSCs is still unclear. Recently it was suggested that GBM stemlike cells with amplified EGFR should be cultured in stem cell medium without EGF, as the presence of EGF induced rapid loss of EGFR amplification. However, patient biopsies are usually taken into culture before their genomic profiles are defined. Thus, an important question remains whether GBM cells without EGFR amplification also can be cultured in stem cell medium without EGF.Meterials and methods. To address this question, we used two heterogeneous glioblastoma GSC lines (NCH421k and NCH644) that lack EGFR amplification.Results. Although both cell lines showed very low EGFR expression under standard growth conditions, bFGF stimulation induced higher expression of EGFR in NCH644. In both cell lines, expression of the stem cell markers nestin and CD133 was higher upon stimulation with bFGF compared to EGF. Importantly, bFGF stimulated the growth of both cell lines, whereas EGF had no effect. We verified that the growth stimulation by bFGF was either mediated by proliferation (NCH421k) or resistance to apoptosis (NCH644).Conclusions. We demonstrate that GSC cultures without EGFR amplification can be maintained and expanded with bFGF, while the addition of EGF has no significant effect and therefore can be omitted.
Ključne besede: glioblastoma, stem cell cultures, bFGF
Objavljeno v DiRROS: 03.04.2024; Ogledov: 1347; Prenosov: 632
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