Iskanje po repozitoriju

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Povzetek: Objectives. Next-generation sequencing (NGS) provide a comprehensive analysis of the genetic alterations that are most commonly linked with pyrazinamide (PZA) resistance. There are no studies reporting molecular background of PZA resistance in TB isolates from Balkan Peninsula. We aimed to examine the feasibility of full-length analysis of a gene linked with PZA resistance, pncA, using Ion Torrent technology in comparison to phenotypic BACTEC MGIT 960 DST in clinical TB isolates from two countries of the Balkan Peninsula. Methods. Between 1996 and 2017, we retrospectively selected 61 TB isolates. To identify gene variants related to drug resistance in genomic DNA extracted from TB isolates, AmpliSeq libraries were generated automatically using the AmpliSeq™ Kit for Chef DL8 and the Ion AmpliSeq TB Research Panel. Result.s Of all 61 TB isolates included, 56 TB were phenotypically resistant to any antibiotic. Among them, 38/56 (67.9%) TB isolates were phenotypically resistant to pyrazinamide and pncA mutations were detected in 33/38 cases (86.8%). A mutation in the pncA promoter region was the most prevalent genetic alteration, detected in eight TB isolates. Comparison of NGS to conventional BACTEC MGIT 960 DST revealed very strong agreement (90.2%) between the two methods in identifying PZA resistance, with high sensitivity (89.5%) and specificity (95.7%) for NGS. Conclusions. Detection of PZA resistance using NGS seems to be a valuable tool for surveillance of TB drug resistance also in the Balkan Peninsula, with great potential to provide useful information at least one weak earlier than is possible with phenotypic DST.
Ključne besede: tuberculosis, Mycobacterium tuberculosis, high-throughput nucleotide sequencing, pyrazinamide, microbial sensitivity tests, next-generation sequencing, drug susceptibility testing, Slovenia, Republic of North Macedonia
Objavljeno v DiRROS: 10.01.2022; Ogledov: 886; Prenosov: 541
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Povzetek: The author examines the Kosovo crisis in the context of the pluralisation and democratisation of Slovenian society in the 1980s and early 1990s. This issue became a catalyst not only for the repositioning of structures of party leadership in relation to Belgrade, but also with respect to general public debates. By charting individual stages of the critical decade of 1981%1991, the author presents Slovenian perceptions of Kosovo%s political, economic, and social issues, first through the works of neo-Marxist critics and later through the activism of a group of left-liberal intellectuals, which included the provision of legal support and a high-profile social action related to the violations of Kosovar Albanian human rights. The author discusses the constraints encountered by this brief attempt to establish a pan-Yugoslav civil society initiative. At the same time, he shows how the complexities of the Kosovo crisis were used to coalesce the Slovenian nation into flight from Yugoslavia.
Ključne besede: self-management socialism, human rights, social cohesion, dissolution of Yugoslavia, Kosovo, Slovenia
Objavljeno v DiRROS: 23.12.2021; Ogledov: 671; Prenosov: 622
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Ključne besede: Poplar, Willow, Black Walnut, Black Locust, international commission, FAO, national report, questionnaire, genetics, forest protection, legislation, categorisation, forest products, ecology, Slovenia
Objavljeno v DiRROS: 11.12.2020; Ogledov: 1319; Prenosov: 411
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Povzetek: The spotted wing drosophila (Drosophila suzukii (Matsumura)) is a highly invasive species and attacking different species of berry carrying hosts. Much research has already been done on the crop hosts over the world and in Slovenia, but for wild hosts less is known. On basis of literature and fieldwork we prepared a list of potential and actual known species of wild hosts for Slovenia. In 2019, berries of different species were collected and D. suzukii was either reared from these berries or berries were dissected. In total we found in the literature for Europe 99 species which were used as host for D. suzukii. For Slovenia we found 71 potential hosts and 14 hosts which were actually infested. in Slovenia there was a broad range of potential hosts from 41 genera. The genera with the most potential hosts were Prunus, Lonicera and Vaccinium. Among the potential hosts were also many species which were invasive alien or alien species. The list was discussed in the context of management implications and further research on D. suzukii in Slovenia.
Ključne besede: spotted wing drosophila, berries, potential hosts, wild hosts, forests, pest control, Slovenia
Objavljeno v DiRROS: 11.12.2020; Ogledov: 1182; Prenosov: 518
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Povzetek: We analyzed variation in the functional composition and diversity of understory plant communities across different forest vegetation types in Slovenia. The study area comprises 10 representative forest sites covering broad gradients of environmental conditions (altitude, geology, light availability, soil type and reaction, nutrient availability, soil moisture), stand structural features and community attributes. The mean and variation of the trait values were quantified by community-weighted means and functional dispersion for four key plant functional traits: plant height, seed mass, specific leaf area and leaf dry matter content. At each study site, forest vegetation was surveyed at two different spatial scales (4 and 100 m2 ) in order to infer scale-dependent assembly rules. Patterns of community assembly were tested with a null model approach. We found that both trait means and diversity values responded to conspicuous gradients in environmental conditions and species composition across the studied forests. Our results mainly support the idea of abiotic filtering: more stressful environmental conditions (e.g., high altitude, low soil pH and low nutrient content) were occupied by communities of low functional diversity (trait convergence), which suggests a selective effect for species with traits adapted to such harsh conditions. However, trait convergence was also detected in some more resource-rich forest sites (e.g., low altitude, high soil productivity), most likely due to the presence of competitive understory species with high abundance domination. This could, at least to some extent, indicate the filtering effect of competitive interactions. Overall, we observed weak and inconsistent patterns regarding the impact of spatial scale, suggesting that similar assembly mechanisms are operating at both investigated spatial scales. Our findings contribute to the baseline understanding of the role of both abiotic and biotic constraints in forest community assembly, as evidenced by the non-random patterns in the functional structure of distinct temperate forest understories.
Ključne besede: functional composition, functional diversity, ecological gradients, abiotic filtering, trait convergence, trait divergence, spatial scale, forest ground-layer vegetation, Slovenia
Objavljeno v DiRROS: 26.05.2020; Ogledov: 1867; Prenosov: 1253
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