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Iskalni niz: "avtor" (Roman Jerala) .

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Postulates for validating TLR4 agonists
Roman Jerala, Mateja Manček Keber, 2015

Povzetek: TLRs play a central role in the innate immune response, recognizing a variety of molecular structures characteristic of pathogens. Although TLR4, together with its co-receptor myeloid differentiation-2 (MD-2), recognize bacterial LPS and therefore Gram-negative bacterial infections, it also plays a key role in many other pathophysiological processes, including sterile inflammation and viral infection. Specifically, numerous endogenous agonists of TLR4 of notably diverse nature, ranging from proteins to metal ions, have been reported. Direct activation of a single receptor by such a range of molecular signals is very difficult to explain from a structural and mechanistic point of view. It is likely that only a subset of these directly activate the TLR4–MD-2 complex. We propose three postulates aimed at distinguishing the direct agonists of TLR4 from indirect activators. These postulates are as follows: (i) that the agonist requires the TLR4/MD-2 receptor complex; (ii) that agonist formed synthetically or in situ must activate the receptor complex in order to eliminate artifacts of contamination by other agonists; and (iii) that a specific molecular interaction between the agonist and TLR4/MD-2 must be identified. The same type of postulates can be applied to pattern recognition receptors in general.
Ključne besede: TLR4 agonists, molecular mechanism, ligand recognition
DiRROS - Objavljeno: 26.01.2015; Ogledov: 3669; Prenosov: 250

Towards designing new nano-scale protein architectures
Jana Aupič, Žiga Strmšek, Fabio Lapenta, Roman Jerala, 2016

DiRROS - Objavljeno: 12.11.2018; Ogledov: 2287; Prenosov: 900
URL Celotno besedilo (0,00 KB)
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Design principles for rapid folding of knotted DNA nanostructures
Roman Jerala, Jonathan P. K. Doye, Tomaž Pisanski, Nino Bašić, Helena Gradišar, Slavko Čeru, John S. Schreck, Vid Kočar, 2016

Povzetek: Knots are some of the most remarkable topological features in nature. Self-assembly of knotted polymers without breaking or forming covalent bonds is challenging, as the chain needs to be threaded through previously formed loops in an exactly defined order. Here we describe principles to guide the folding of highly knotted single-chain DNA nanostructures as demonstrated on a nano-sized square pyramid. Folding of knots is encoded by the arrangement of modules of different stability based on derived topological and kinetic rules. Among DNA designs composed of the same modules and encoding the same topology, only the one with the folding pathway designed according to the "free-end" rule folds efficiently into the target structure. Besides high folding yield on slow annealing, this design also folds rapidly on temperature quenching and dilution from chemical denaturant. This strategy could be used to design folding of other knotted programmable polymers such as RNA or proteins.
DiRROS - Objavljeno: 13.04.2016; Ogledov: 3125; Prenosov: 566
URL Celotno besedilo (0,00 KB)

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