1. The Beginnings of Slovene Germanic Philology : the First Professors and the Social Profile of the First Students from the LittoralIrena Samide, Petra Kramberger, 2023, izvirni znanstveni članek Povzetek: This article seeks to shed light on the beginnings of Germanic philology in the Slovene lands, concentrating both on the leading figure of Dr Jakob Kelemina (1882–1957), who established Germanic philology in the Slovene academic space and dominated it for many years, and on the profile of his students from the Slovene, Italian, and Croatian littoral. The findings, which are based on the quantitative and qualitative analysis of archive sources, lecture lists and student records, and on Bourdieu’s classification of education opportunities by economic and cultural capital, show that students from the coastal regions made up 17% of the 916 students of both sexes who attended lectures on Germanic philology up until the end of the Second World War; of these, 54% were women. The students’ social origin was found to have had considerable influence on their decision to take up such studies, as the large majority came from the middle and upper strata of the population.
Ključne besede: Germanic philology, student profile, students from the Littoral, history of Germanic philology 1919–1945, cultural capital
Objavljeno v DiRROS: 08.07.2026; Ogledov: 158; Prenosov: 171
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2. Methacrylate monolith chromatography as a tool for waterborne virus removalNejc Rački, Petra Kramberger, Andrej Steyer, Jernej Gašperšič, Aleš Štrancar, Maja Ravnikar, Ion Gutiérrez-Aguirre, 2015, izvirni znanstveni članek Povzetek: Enteric viruses are commonly present in environmental waters and represent the major cause of waterborne infections and outbreaks. Since traditional wastewater treatments fail to remove enteric viruses in the water purification process, they are released daily into environmental waters. Monolithic supports have enabled chromatography to enter the field of virology. They have been successfully used in virus purification and concentration. In this work quaternary amine (QA) methacrylate monoliths were exploited to remove enteric viruses from wastewater treatment plant effluent. Expectedly, chromatographic processing of such a complex medium was troublesome, even for monoliths, characterized by extremely large pore dimensions. This problem was solved by introducing a pre-step chromatography using hydroxyl (OH) methacrylate monoliths. This way, molecules, that would hinder virus binding to the anion-exchanger monolith, were removed. As a result, the OH pre-column reduced backpressure increase on the subsequent anion-exchanger column, and increased both QA column binding capacity and life time. Wastewater effluent samples were successfully purified from five waterborne enteric viruses (rotavirus, norovirus genogroup I and II, astrovirus, sapovirus), below the detection limit of RT-qPCR. The breakthrough of the rotavirus binding capacity was not reached for concentrations that significantly exceeded those expected in effluent waters. The obtained results confirm that methacrylate monoliths can be a valuable tool for simultaneous removal of different waterborne viruses from contaminated water sources.
Ključne besede: monolith chromatography, waterborne, virus, removal, wastewater, qPCR
Objavljeno v DiRROS: 26.07.2024; Ogledov: 1612; Prenosov: 787
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3. Filling the gaps in diagnostics of Pepino mosaic virus and Potato spindle tuber viroid in water and tomato seeds and leavesNataša Mehle, Polona Kogovšek, Nejc Rački, Tjaša Jakomin, Ion Gutiérrez-Aguirre, Petra Kramberger, Maja Ravnikar, 2017, izvirni znanstveni članek Povzetek: Waterborne and seedborne Pepino mosaic virus (PepMV) and Potato spindle tuber viroid (PSTVd) pose serious threats to tomato production due to seed transmission and mechanical transmission, coupled with their long-term stability outside the host plant. Therefore, rapid and sensitive diagnostic procedures are needed to prevent the spread of these quarantine pathogens. In particular, water and seed contamination are difficult to detect and confirm without efficient concentration methods. This study presents procedures that improve detection of PSTVd from tomato seeds and leaf tissue, and PepMV from water and tomato leaf tissue. For efficient concentration of PepMV from water samples, a procedure was optimized using convective interaction media monolithic chromatography columns, which provides concentration by three orders of magnitude. For concentration of PSTVd from seed extracts, an easy-to-use and efficient method was developed based on RNA binding to positively charged anion-exchange resin beads that provides up to 100-fold more sensitive detection in comparison with procedures without a concentration step. This thus allows confirmation of RT-qPCR results with sequencing of RT-PCR products in samples with low viroid levels. In addition, reverse-transcription loop-mediated isothermal amplification assays for detection of PSTVd and PepMV were optimized and adapted to both laboratory and on-site testing requirements. This allows rapid detection of these pathogens in crude leaf homogenates, in under 30 min. These procedures of concentration and detection are shown to be efficient and to fill the gaps in diagnostics of PepMV and PSTVd, especially when these pathogens are present at low levels in difficult matrices such as water and seeds.
Ključne besede: PSTVd, PepMV, seeds, water, concentration, loop-mediated isothermal amplification
Objavljeno v DiRROS: 24.07.2024; Ogledov: 1766; Prenosov: 877
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