1. In vitro genotoxicity assessment of commercially available graphene quantum dots in human peripheral blood cells and salivary leukocytesTamara Ćetković Pećar, Irma Durmišević, Mirta Milić, Anja Haverić, Bojana Žegura, 2026, izvirni znanstveni članek Povzetek: Commercially available graphene quantum dots (GQDs) are promising nanomaterials for applications in research and preclinical diagnostics, drug delivery, and bioimaging. Their bioactivity is highly dependent on dose, route of exposure, duration, cell type, uptake mechanisms, tissue and cellular distribution, and physicochemical properties. This study aimed to evaluate genotoxic, cytotoxic, and cytostatic endpoints of blue- (B-GQDs) and greenemitting (G-GQDs) GQDs in human blood and salivary leukocytes. GQDs were tested at concentrations ranging from 2.5 to 100 μg/mL using distinct treatment periods. Fourier transform infrared spectroscopy (FTIR), trypan blue exclusion, comet, and cytokinesisblock micronucleus cytome (CBMN cyt) assays were performed. FTIR analysis revealed that G-GQDs, unlike B-GQDs, exhibit an absorption band typically associated with amine functional groups, which may contribute to their pronounced genotoxic effects. Peripheral blood mononuclear cells and salivary leukocytes showed higher sensitivity to G-GQDs compared to whole blood samples. Although no cytotoxic effects were observed, both GQDs induced significant DNA damage, with G-GQDs demonstrating greater genotoxic potential. These findings demonstrate that GQDs can induce DNA damage in the absence of detectable cytotoxic effects under the conditions tested, highlighting the importance of considering both physicochemical properties and cellular models in the safety assessment of nanomaterials.
Ključne besede: GQDs, FTIR, DNA damage, cell viability, micronuclei
Objavljeno v DiRROS: 23.06.2026; Ogledov: 237; Prenosov: 191
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2. Data from: Cytogenotoxic effects of polycyclic aromatic hydrocarbons complex mixture in human peripheral blood, lung A549 and liver HepG2 cells : translation of a real-scenario exposure to in vitroLuka Kazensky, Marija Jelena Lovrić Štefiček, Vilena Kašuba, Matjaž Novak, Karolina Belingar, Katarina Matković, Marko Gerić, Jasmina Rinkovec, Ivana Jakovljević, Katarina Baralić, Danijela Đukić-Ćosić, Mirta Milić, Želimir Jelčić, Gordana Pehnec, Bojana Žegura, Goran Gajski, 2026, zaključena znanstvena zbirka raziskovalnih podatkov Ključne besede: cytotoxicity, genomic instability, in silico, indoor air pollution, public health
Objavljeno v DiRROS: 16.02.2026; Ogledov: 227; Prenosov: 174
 Raziskovalni podatki (11,36 MB)
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3. Minimum Information for Reporting on the Comet Assay (MIRCA) : recommendations for describing comet assay procedures and resultsPeter Møller, Amaya Azqueta, Elisa Boutet-Robinet, Gudrun Koppen, Stefano Bonassi, Mirta Milić, Goran Gajski, Solange Costa, Bojana Žegura, Matjaž Novak, 2020, izvirni znanstveni članek Povzetek: The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between ‘desirable’ and ‘essential’ information: ‘essential’ information refers to the precise details that are necessary to assess the quality of the experimental work, whereas ‘desirable’ information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.
Objavljeno v DiRROS: 22.07.2024; Ogledov: 2052; Prenosov: 868
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4. DNA damage in circulating leukocytes measured with the comet assay may predict the risk of deathStefano Bonassi, Marcello Ceppi, Peter Møller, Amaya Azqueta, Mirta Milić, Gunnar Brunborg, Gudrun Koppen, Marco Bruzzone, Juliana Da Silva, Danieli Benedetti, Silvia Moretti, Patrizia Riso, Patrizia Russo, Ricardo Marcos, Goran Gajski, Biljana Spremo-Potparević, Lada Živković, Elisa Boutet-Robinet, Maria Dusinska, Andrew Collins, Bojana Žegura, 2021, izvirni znanstveni članek Povzetek: The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
Objavljeno v DiRROS: 19.07.2024; Ogledov: 1560; Prenosov: 851
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