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Iskalni niz: "avtor" (Miran Mozetič) .

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Improved adhesion and biocompatibility of chitosan-coated super-hydrophilic PVC polymer substrates for urothelial catheters
Alenka Vesel, Helena Motaln, Miran Mozetič, Dane Lojen, Nina Recek, 2025, izvirni znanstveni članek

Povzetek: Chitosan is a water-soluble polysaccharide with good adherence to negatively charged surfaces and reported antimicrobial and anti-inflammatory properties. Coating the surfaces of medical devices with chitosan is a promising strategy for harnessing these benefits. However, the surface properties of commercial polymers need to be altered to enable the bonding of thin chitosan films. In this study, the adhesion of chitosan onto plasma-treated polyvinyl chloride (PVC) and the metabolic activity of urothelial cells on chitosan-coated medical-grade PVC used for the synthesis of urinary catheters were evaluated. To improve the adhesion of chitosan onto the PVC catheters, PVC samples were made “super-hydrophilic”. PVC substrates were briefly treated with a powerful hydrogen plasma and weakly ionised oxygen plasma afterglow to obtain a chlorine-free surface film, which was rich in oxygen functional groups, followed by incubation of the plasma-treated substrates in an aqueous solution of chitosan. Then, urothelial RT4 cells were seeded on the treated and untreated PVC substrates, and their metabolic activity, confluency, and cell morphology were examined. X-ray photoelectron spectroscopy was used to measure the nitrogen concentration, which corresponded to the chitosan concentration on the substrate. The results showed that the substrates were uniformly covered by a thin layer of chitosan only on plasma-treated surfaces and not on untreated surfaces. Moreover, the chitosan coating provided a stimulated environment for cell adhesion and growth. In conclusion, the chitosan-coated super-hydrophilic PVC substrate shows potential to improve the overall performance and safety of medical devices such as urinary catheters.
Ključne besede: medical-grade PVC, chitosan coating, urinary catheter, urothelial cells, cytotoxicity, adhesion, biocompatibility
Objavljeno v DiRROS: 28.02.2025; Ogledov: 241; Prenosov: 137
.pdf Celotno besedilo (14,65 MB)
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3.
Vacuum ultraviolet radiation from gaseous plasma for destruction of water contaminants
Mark Zver, Rok Zaplotnik, Miran Mozetič, Alenka Vesel, Arijana Filipić, David Dobnik, Belisa Alcantara Marinho, Gregor Primc, 2025, izvirni znanstveni članek

Povzetek: Innovative technological solutions are needed for water decontamination to combat the diverse pollutants present in water systems, as no single optimal decontamination technique is appropriate for all circumstances. Vacuum-ultraviolet (V-UV) radiation is a source of energetic photons that break molecular bonds, producing a plethora of chemically reactive agents, most notably OH● radicals, which can cause the degradation of harmful pollutants. Low-pressure gaseous plasma is a good source of V-UV radiation; however, its application to liquid water poses challenges. We constructed an inductively coupled radiofrequency plasma to produce high-intensity V-UV radiation, which was applied to contaminated water via a V-UV-transparent window. Plasma was sustained in hydrogen, as it produces the highest V-UV intensity among all gases at selected discharge parameters. Bacteriophage MS2 was used as an indicator of microbial decontamination efficiency. Reactive oxygen and nitrogen species were measured at various treatment setups to quantify their effect on MS2 inactivation and elucidate the primary inactivation factors. At optimal conditions, the concentration of active virus dropped by 9 log10 PFU/mL in 60 s. The optimal experimental setup was then used to treat bacteria E. coli, S. aureus, antibiotic tetracycline, and synthetic dye methylene blue as representatives of other types of pollutants, all of which were effectively removed/degraded within 10 min of treatment. A comparison of energy efficiency (EEO) to other disinfection setups was made for bacteriophage inactivation. With a low EEO value, we showcase the potential of this technique for further work in this field.
Ključne besede: water treatment, radical
Objavljeno v DiRROS: 07.02.2025; Ogledov: 282; Prenosov: 149
.pdf Celotno besedilo (6,71 MB)
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Cold plasma within a stable supercavitation bubble - a breakthrough technology for efficient inactivation of viruses in water
Arijana Filipić, David Dobnik, Ion Gutiérrez-Aguirre, Maja Ravnikar, Tamara Košir, Špela Baebler, Alja Štern, Bojana Žegura, Martin Petkovšek, Matevž Dular, Miran Mozetič, Rok Zaplotnik, Gregor Primc, 2023, izvirni znanstveni članek

Povzetek: Water scarcity, one of the most pressing challenges we face today, has developed for many reasons, including the increasing number of waterborne pollutants that affect the safety of the water environment. Waterborne human, animal and plant viruses represent huge health, environmental, and financial burden and thus it is important to efficiently inactivate them. Therefore, the main objective of this study was to construct a unique device combining plasma with supercavitation and to evaluate its efficiency for water decontamination with the emphasis on inactivation of viruses. High inactivation (>5 log10 PFU/mL) of bacteriophage MS2, a human enteric virus surrogate, was achieved after treatment of 0.43 L of recirculating water for up to 4 min. The key factors in the inactivation were short-lived reactive plasma species that damaged viral RNA. Water treated with plasma for a short time required for successful virus inactivation did not cause cytotoxic effects in the in vitro HepG2 cell model system or adverse effects on potato plant physiology. Therefore, the combined plasma-supercavitation device represents an environmentally-friendly technology that could provide contamination-free and safe water.
Ključne besede: cold plasma, hydrodynamic cavitation, supercavitation, virus inactivation, water decontamination, toxicity assays
Objavljeno v DiRROS: 28.11.2024; Ogledov: 391; Prenosov: 589
.pdf Celotno besedilo (4,15 MB)
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6.
Effects of hydrogen dissociation during gas flooding on formation of metal hydride cluster ions in secondary ion mass spectrometry
Jernej Ekar, Sabina Markelj, Miran Mozetič, Rok Zaplotnik, Janez Kovač, 2024, izvirni znanstveni članek

Povzetek: The application of hydrogen flooding was recently shown to be a simple and effective approach for improved layer differentiation and interface determination during secondary ion mass spectrometry (SIMS) depth profiling of thin films, as well as an approach with potential in the field of quantitative SIMS analyses. To study the effects of hydrogen further, flooding of H2 molecules was compared to reactions with atomic H on samples of pure metals and their alloys. H2 was introduced into the analytical chamber via a capillary, which was heated to approximately 2200 K to achieve dissociation. Dissociation of H2 up to 30% resulted in a significant increase in the intensity of the metal hydride cluster secondary ions originating from the metallic samples. Comparison of the time scales of possible processes provided insight into the mechanism of hydride cluster secondary ion formation. Cluster ions presumably form during the recombination of the atoms and molecules from the sample and atoms and molecules adsorbed from the gas. This process occurs on the surface or just above it during the sputtering process. These findings coincide with those of previous mechanistic and computational studies.
Ključne besede: hydrogen atmosphere, molecule dissociation, gas flooding, cluster ions, secondary ion mass spectrometry
Objavljeno v DiRROS: 08.11.2024; Ogledov: 403; Prenosov: 224
.pdf Celotno besedilo (1,18 MB)
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7.
Antiviral respiratory masks with plasma-functionalized polypropylene textiles for optimal adsorption of antiviral substance
Mark Zver, David Dobnik, Rok Zaplotnik, Miran Mozetič, Alenka Vesel, Arijana Filipić, Polona Kogovšek, Katja Fric, Alja Štern, Gregor Primc, 2024, izvirni znanstveni članek

Povzetek: During the COVID-19 pandemic, face masks were the first line of defense against the spread of infection. However, infectious viruses may remain on medical textiles, potentially serving as an additional source of infection. Due to their chemical inertness, many textiles cannot be enhanced with antiviral functionalities. Through treatment with low-pressure gaseous plasma, we have activated the surface of a medical-grade melt-blown, non-woven polypropylene textile so that it can absorb sodium dodecyl sulfate, an antimicrobial surfactant. Within two hours of contact time, the functionalized textile has been able to inactivate over 7 log10 PFU mL−1 of bacteriophage phi6, a surrogate of enveloped viruses such as SARS-CoV-2, and it has retained its antiviral properties for over 100 days. The functionalized material has not disrupted facial mask filtration efficiency or breathability. In addition, the in vitro biocompatibility testing in accordance with ISO 10993-5 for testing of medical devices has demonstrated that the selected formulation causes no adverse effects on the mouse fibroblast cell line L-929. With the treatment processes that have been completed within seconds, the method seems to have great potential to produce antiviral textiles against future outbreaks.
Ključne besede: surgical face masks, plasma functionalization, antiviral materials, virus filtration, breathability
Objavljeno v DiRROS: 07.10.2024; Ogledov: 544; Prenosov: 526
.pdf Celotno besedilo (4,16 MB)
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8.
Cold plasma, a new hope in the field of virus inactivation
Arijana Filipić, Ion Gutiérrez-Aguirre, Gregor Primc, Miran Mozetič, David Dobnik, 2020, pregledni znanstveni članek

Povzetek: Viruses can infect all cell-based organisms, from bacteria to humans, animals, and plants. They are responsible for numerous cases of hospitalization, many deaths, and widespread crop destruction, all of which result in an enormous medical, economical, and biological burden. Each of the currently used decontamination methods has important drawbacks. Cold plasma (CP) has entered this field as a novel, efficient, and clean solution for virus inactivation. We present recent developments in this promising field of CP-mediated virus inactivation, and describe the applications and mechanisms of the inactivation. This is particularly relevant because viral pandemics, such as COVID-19, highlight the need for alternative virus inactivation methods to replace, complement, or upgrade existing procedures.
Objavljeno v DiRROS: 06.08.2024; Ogledov: 684; Prenosov: 391
.pdf Celotno besedilo (1,92 MB)
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9.
Cell proliferation on polyethylene terephthalate treated in plasma created in ▫$SO_2/O_2$▫ mixtures
Nina Recek, Matic Resnik, Rok Zaplotnik, Miran Mozetič, Helena Motaln, Tamara Lah Turnšek, Alenka Vesel, 2017, izvirni znanstveni članek

Povzetek: Samples of polymer polyethylene terephthalate were exposed to a weakly ionized gaseous plasma to modify the polymer surface properties for better cell cultivation. The gases used for treatment were sulfur dioxide and oxygen of various partial pressures. Plasma was created by an electrodeless radio frequency discharge at a total pressure of 60 Pa. X-ray photoelectron spectroscopy showed weak functionalization of the samples’ surfaces with the sulfur, with a concentration around 2.5 at %, whereas the oxygen concentration remained at the level of untreated samples, except when the gas mixture with oxygen concentration above 90% was used. Atomic force microscopy revealed highly altered morphology of plasma-treated samples; however, at high oxygen partial pressures this morphology vanished. The samples were then incubated with human umbilical vein endothelial cells. Biological tests to determine endothelialization and possible toxicity of the plasma-treated polyethylene terephthalate samples were performed. Cell metabolic activity (MTT) and in vitro toxic effects of unknown compounds (TOX) were assayed to determine the biocompatibility of the treated substrates. The biocompatibility demonstrated a well-pronounced maximum versus gas composition which correlated well with development of the surface morphology.
Objavljeno v DiRROS: 25.07.2024; Ogledov: 549; Prenosov: 453
.pdf Celotno besedilo (10,82 MB)
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10.
Cold atmospheric plasma as a novel method for inactivation of potato virus Y in water samples
Arijana Filipić, Gregor Primc, Rok Zaplotnik, Nataša Mehle, Ion Gutiérrez-Aguirre, Maja Ravnikar, Miran Mozetič, Jana Žel, David Dobnik, 2019, izvirni znanstveni članek

Povzetek: While one of the biggest problems we are facing today is water scarcity, enormous quantities of water are still being used in irrigation. If contaminated, this water can act as an effective pathway for the spread of disease-causing agents, like viruses. Here, we present a novel, environmentally friendly method known as cold atmospheric plasma for inactivation of viruses in water used in closed irrigation systems. We measured the plasma-mediated viral RNA degradation as well as the plasma-induced loss of viral infectivity using potato virus Y as a model virus due to its confirmed water transmissibility and economic as well as biological importance. We showed that only 1 min of plasma treatment is sufficient for successful inactivation of viruses in water samples with either high or low organic background. The plasma-mediated inactivation was efficient even at markedly higher virus concentrations than those expected in irrigation waters. Obtained results point to reactive oxygen species as the main mode of viral inactivation. Our laboratory-scale experiments confirm for the first time that plasma has an excellent potential as the eukaryotic virus inactivation tool for water sources and could thus provide a cost-effective solution for irrigation mediated plant virus transmission. The outstanding inactivation efficiency demonstrated by plasma treatments in water samples offers further expansions of its application to other water sources such as reused wastewater or contaminated drinking waters, as well as other plant, animal, and human waterborne viruses, ultimately leading to the prevention of water scarcity and numerous human, animal, and plant infections worldwide.
Ključne besede: cold atmospheric plasma, potato virus Y, virus inactivation, water decontamination
Objavljeno v DiRROS: 23.07.2024; Ogledov: 597; Prenosov: 359
.pdf Celotno besedilo (985,33 KB)
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