1. Measuring biological age : insights from omics studiesEva Kočar, Robert Šket, Ana Halužan Vasle, Gorazd Avguštin, Evgen Benedik, Barbara Koroušić-Seljak, Pavle Simić, Antonio Martinko, Shawnda A. Morrison, Maroje Sorić, Mihaela Skrt, Tomaž Polak, Tine Tesovnik, Barbara Jenko Bizjan, Jernej Kovač, Tadej Battelino, Damjana Rozman, Nataša Poklar Ulrih, Bojana Bogovič Matijašić, Gregor Jurak, Miha Moškon, Tadeja Režen, 2026, pregledni znanstveni članek Povzetek: Biological ageing is a systemic, multifactorial process driven by progressive molecular and cellular alterations whose complexity necessitates systems-level approaches. Advances in high-throughput omics technologies now allow simultaneous quantification of millions of biomolecules from a single specimen, enabling longitudinal, integrative profiling across multiple molecular layers. This review synthesizes recent progress in applying genomics, epigenomics, metabolomics and microbiomics to ageing research, highlighting their contributions to biomarker discovery, mechanistic insight, and translational opportunities. Genomic studies reveal genetic variants that promote extreme longevity, while epigenetic clocks provide robust predictors of biological age. The blood proteome can be used to calculate proteome-based scores and evaluate temporal changes in ageing trajectories in an organ- and sex-specific manner. Metabolomic signatures identify key metabolites reflecting ageing trajectories, and microbiome research demonstrates that gut microbial composition mirrors and modulates biological ageing, with microbiome clocks emerging. The omics approaches have further elucidated the impact of exercise and diet providing evidence that interventions can reduce biological age. The integration of multi-omics with clinical and lifestyle data, powered by machine learning and artificial intelligence, is paving the way for a holistic definition of biological age and the development of personalized healthy ageing strategies. This review highlights how the omics technologies and computational modelling are transforming ageing biology into strategies for personalized healthy ageing.
Ključne besede: ageing, biological ageing, omics, physical fitness, nutrition, computational modelling
Objavljeno v DiRROS: 08.01.2026; Ogledov: 824; Prenosov: 356
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2. Stabilisation of lutein and lutein esters with polyoxyethylene sorbitan monooleate, medium-chain triglyceride oil and lecithinZala Gombač, Ilja Gasan Osojnik Črnivec, Mihaela Skrt, Katja Istenič, Andreja Knez Knafelj, Igor Pravst, Nataša Poklar Ulrih, 2021, izvirni znanstveni članek Objavljeno v DiRROS: 11.11.2025; Ogledov: 433; Prenosov: 313
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3. Verifying the use of food labelling data for compiling branded food databases : a case study of sugars in beveragesEdvina Hafner, Živa Lavriša, Maša Hribar, Sanja Krušič, Anita Kušar, Katja Žmitek, Mihaela Skrt, Nataša Poklar Ulrih, Igor Pravst, 2022, izvirni znanstveni članek Ključne besede: beverages, reformulation, added sugar, food composition, HPLC, database
Objavljeno v DiRROS: 11.11.2025; Ogledov: 569; Prenosov: 306
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4. Nano spray-dried particles of in-situ crosslinked alginate and their toxicological characterisationJaka Levanič, Ilja Gasan Osojnik Črnivec, Iza Rozman, Mihaela Skrt, Alja Štern, Bojana Žegura, Nataša Poklar Ulrih, 2024, izvirni znanstveni članek Povzetek: The feasibility and technical capacity for producing crosslinked sub-micron gels with a nano spray-dryer were studied with variable pH systems incorporating alginate, pectin, and pullulan. The obtained powders were characterized for their morphology, particle size distribution, and their toxicological safety profile using genotoxicity and cytotoxicity assays. Additionally, quercetin was added to the encapsulation system to study the potential of the system to encapsulate this material. The produced powders exhibited morphologies and particle size distributions characteristic for nano spray-drying. The addition of pullulan and pectin to the feed solutions resulted in a particle size increase, with crosslinked alginate particles having a mean value of 1.43 μm, while particles with added pectin and pullulan had a mean particle size of 1.70 and 1.75 μm, respectively. The inclusion of quercetin proved to be problematic with this encapsulation system. Extremely high degradation rates and extremely low encapsulation efficiencies were observed due to the alkaline pH (~10) of the system that is needed to keep the feed dispersion in a liquid state and prevent premature crosslinking of the alginate. Although pectin and pullulan provided some protection for quercetin in the alkaline dispersion, the absolute quercetin content in the final product remained very low, with a maximum achieved encapsulation efficiency of 2.06 %. The safety profile of most produced powders was favourable, as they did not exhibit any significant cytotoxic and genotoxic activity in the HepG2 cell line, except in the case of Alginate/Pullulan which showed a 43 % decrease in cell viability at 500 μg/mL. Samples where quercetin was added did not show any increased toxicological effect.
Ključne besede: alginate, polysaccharides, crosslinking, nano spray drying, toxicological evaluation
Objavljeno v DiRROS: 08.05.2025; Ogledov: 1062; Prenosov: 809
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