1. Metadata from: Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal regionAnže Vozelj, Tjaša Mahkovec Povalej, Katja Stare, Magda Tušek-Žnidarič, Katarina Bačnik, Valentina Levak, Ion Gutiérrez-Aguirre, Marjetka Podobnik, Kristina Gruden, Anna Coll Rius, Tjaša Lukan, 2025, raziskovalni podatki Ključne besede: potato virus Y, coat protein, viral movement, point mutations, Solanum tuberosum
Objavljeno v DiRROS: 31.03.2026; Ogledov: 435; Prenosov: 474
 Celotno besedilo (4,42 KB)
Gradivo ima več datotek! Več... |
2. High-throughput human histone detection by an engineered actinoporin nanoporeMarija Srnko, Gašper Šolinc, Ana Crnković, Franci Merzel, Michael Jordan, E. Jayne Wallace, Marjetka Podobnik, Gregor Anderluh, 2026, izvirni znanstveni članek Objavljeno v DiRROS: 30.03.2026; Ogledov: 459; Prenosov: 714
 Celotno besedilo (3,94 MB)
Gradivo ima več datotek! Več... |
3. Species-specific structural adaptation of the potyviral coat protein in virions and virus-like particlesNeža Koritnik, Andreja Kežar, Luka Kavčič, Magda Tušek-Žnidarič, Adrijana Leonardi, Swarnalok De, Maija Pollari, Kristiina Mäkinen, Marjetka Podobnik, 2026, izvirni znanstveni članek Objavljeno v DiRROS: 20.03.2026; Ogledov: 524; Prenosov: 347
Celotno besedilo (4,18 MB)
Gradivo ima več datotek! Več... |
4. From structural polymorphism to structural metamorphosis of the coat protein of flexuous filamentous potato virus YLuka Kavčič, Andreja Kežar, Neža Koritnik, Magda Tušek-Žnidarič, Tajda Klobučar, Žiga Vičič, Franci Merzel, Ellie Holden, Justin L. P. Benesch, Marjetka Podobnik, 2024, izvirni znanstveni članek Objavljeno v DiRROS: 09.03.2026; Ogledov: 522; Prenosov: 391
Celotno besedilo (5,23 MB)
Gradivo ima več datotek! Več... |
5. Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal regionAnže Vozelj, Tjaša Mahkovec Povalej, Katja Stare, Magda Tušek-Žnidarič, Katarina Bačnik, Valentina Levak, Ion Gutiérrez-Aguirre, Marjetka Podobnik, Kristina Gruden, Anna Coll Rius, Tjaša Lukan, 2025, izvirni znanstveni članek Povzetek: Potato virus Y (PVY) is one of the top 10 economically most important plant viruses and responsible for major yield losses. We previously suggested the involvement of the N-terminal region of PVY coat protein (CP) in PVY spread. By constructing different N-terminal deletion mutants of the PVY N605 strain, we here show that deletions of 40 or more amino acid residues from the N-terminal region of the CP resulted in the PVY multiplication limited to primary infected cells in Nicotiana clevelandii plants. Deletion of 26 residues profoundly impaired PVY cell-to-cell movement and prevented systemic PVY spread, while deletions of 19-23 residues allowed delayed systemic PVY spread. Introduced point mutations in the identified region prevent (S21G) or delay (G20P) PVY movement. In summary, this work shows the significance of the CP N-terminus for movement of the PVY.
Ključne besede: potato virus Y, potato, coat proteins, viral movement, point mutations
Objavljeno v DiRROS: 18.12.2025; Ogledov: 1227; Prenosov: 643
Celotno besedilo (1,49 MB)
Gradivo ima več datotek! Več...
Gradivo je zbirka in zajema 1 gradivo! |
6. Cryo-EM structures of a protein pore reveal a cluster of cholesterol molecules and diverse roles of membrane lipidsGašper Šolinc, Marija Srnko, Franci Merzel, Ana Crnković, Mirijam Kozorog, Marjetka Podobnik, Gregor Anderluh, 2025, izvirni znanstveni članek Povzetek: The structure and function of membrane proteins depend on their interactions with lipids that constitute membranes. Actinoporins are α-pore-forming proteins that bind preferentially to sphingomyelin-containing membranes, where they oligomerize and form transmembrane pores. Through a comprehensive cryo-electron microscopic analysis of a pore formed by an actinoporin Fav from the coral Orbicella faveolata, we show that the octameric pore interacts with 112 lipids in the upper leaflet of the membrane, reveal the roles of lipids, and demonstrate that the actinoporin surface is suited for binding multiple receptor sphingomyelin molecules. When cholesterol is present in the membrane, it forms a cluster of four molecules associated with each protomer. Atomistic simulations support the structural data and reveal additional effects of the pore on the lipid membrane. These data reveal a complex network of protein-lipid and lipid-lipid interactions and an underrated role of lipids in the structure and function of transmembrane protein complexes.
Objavljeno v DiRROS: 12.05.2025; Ogledov: 1231; Prenosov: 632
Celotno besedilo (2,01 MB)
Gradivo ima več datotek! Več... |
7. In vitro evolution driven by epistasis reveals alternative cholesterol-specific binding motifs of perfringolysin OAleksandra Šakanović, Nace Kranjc, Neža Omersa, Saša Aden, Andreja Kežar, Matic Kisovec, Apolonija Bedina Zavec, Simon Caserman, Robert J. Gilbert, Marjetka Podobnik, Ana Crnković, Gregor Anderluh, 2024, izvirni znanstveni članek Objavljeno v DiRROS: 12.09.2024; Ogledov: 1502; Prenosov: 1350
Celotno besedilo (3,73 MB)
Gradivo ima več datotek! Več... |
8. Sequestration of membrane cholesterol by cholesterol-binding proteins inhibits SARS-CoV-2 entry into Vero E6 cellsMagdalena Kulma, Aleksandra Šakanović, Apolonija Bedina Zavec, Simon Caserman, Neža Omersa, Gašper Šolinc, Sara Orehek, Iva Hafner Bratkovič, Urška Kuhar, Brigita Slavec, Uroš Krapež, Matjaž Ocepek, Toshihide Kobayashi, Katarzyna Kwiatkowska, Roman Jerala, Marjetka Podobnik, Gregor Anderluh, 2024, izvirni znanstveni članek Objavljeno v DiRROS: 05.08.2024; Ogledov: 1690; Prenosov: 1129
Celotno besedilo (8,56 MB)
Gradivo ima več datotek! Več... |
9. Protein gas vesicles of bacillus megaterium as enhancers of ultrasound-induced transcriptional regulationVid Jazbec, Nina Varda, Ernest Šprager, Maja Meško, Sara Vidmar, Rok Romih, Marjetka Podobnik, Andreja Kežar, Roman Jerala, Mojca Benčina, 2024, izvirni znanstveni članek Objavljeno v DiRROS: 01.08.2024; Ogledov: 1640; Prenosov: 1180
Celotno besedilo (1,47 MB)
Gradivo ima več datotek! Več... |
10. Structural basis for the multitasking nature of the potato virus Y coat proteinAndreja Kežar, Luka Kavčič, Martin Pólak, Jiři Nováček, Ion Gutiérrez-Aguirre, Magda Tušek-Žnidarič, Anna Coll Rius, Katja Stare, Kristina Gruden, Maja Ravnikar, David Pahovnik, Ema Žagar, Franci Merzel, Gregor Anderluh, Marjetka Podobnik, 2019, izvirni znanstveni članek Povzetek: Potato virus Y (PVY) is among the most economically important plant pathogens. Using cryoelectron microscopy, we determined the near-atomic structure of PVY’s flexuous virions, revealing a previously unknown lumenal interplay between extended carboxyl-terminal regions of the coat protein units and viral RNA. RNA–coat protein interactions are crucial for the helical configuration and stability of the virion, as revealed by the unique near-atomic structure of RNA-free virus-like particles. The structures offer the first evidence for plasticity of the coat protein’s amino- and carboxyl-terminal regions. Together with mutational analysis and in planta experiments, we show their crucial role in PVY infectivity and explain the ability of the coat protein to perform multiple biological tasks. Moreover, the high modularity of PVY virus-like particles suggests their potential as a new molecular scaffold for nanobiotechnological applications.
Ključne besede: plant pathogens, potato virus Y, viral RNA
Objavljeno v DiRROS: 23.07.2024; Ogledov: 1913; Prenosov: 1117
Celotno besedilo (4,43 MB)
Gradivo ima več datotek! Več... |
