11. Cathepsin D and plasminogen activator inhibitor type 1 in normal, benign and alignant ovarian tissues : a preliminary reportMarina Šprem, Damir Babić, Marija Abramić, Duško Miličić, Ivan Vrhovec, Janez Škrk, Maja Osmak, 2000, izvirni znanstveni članek Povzetek: Background. The aim of the present study was to determine the concentration ofcathepsin D (Cath D) and plasminogen activator inhibitor type 1 (PAI-1) in normal ovarian tissues, benign and malignant ovarian tumor tissues, and to asses relationship between Cath D and PAI-1 content, and some clinical and pathohistological parameters. Materials and methods. Cath D contents and PAI-1concentrations were determined (using immunoradiometric ELSA-Cath D assayand commercial IMUDIND R ELISA immunoassay, respectively) in 35 samples: 10 normal ovarii, 10 benign, 10 primary malignant and 5 metastatic ovarian tumors. Results. The concentrations of Cath D were significantly higher in malignant (32.89+-14.26 pmol/mg protein ) and metastatic (31.42+-10.24 pmol/mgprotein), than in normal (13.68+-4.03 pmol/mg protein) and benign (17.89+-13.13 pmol/mg protein) ovarian tissues. There was no statistical differences in the concentrations of PAI-1 between normal, benign, malignant and metastatic tumor specimens. The concentrations of Cath D as well as PAI-1 did not correlate to the age of patients, menopausal status, parity, GOG risk group, clinical stage or pathohistological grading. Conclusion. Concentrationsof Cath D (but not PAI-1) were significantly increased in malignant and metastatic ovarian tumor tissues when compared to normal and benign ovarian tumor samples; they were independent from pathohistological andclinical parameters. Objavljeno v DiRROS: 23.01.2024; Ogledov: 132; Prenosov: 51 Celotno besedilo (442,49 KB) |
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13. Image cytometry analysis of normal buccal mucosa smears: influence of smoking and sex-related differencesJaka Lavrenčak, Margareta Strojan Fležar, Mario Žganec, Marija Us-Krašovec, 1998, izvirni znanstveni članek Povzetek: To get more information about the influence of smoking on chromatin pattern ofnormal buccal mucosa cells and to assess sex-related differences in nuclear features, quantitative analysis of normal buccal mucosa smears was performed. In this study, buccal smears were collected from 78 healthy subjects. Image cytometry analysis was performed on Feulgen-thionin stained smears. probability distributions of 78 nuclear features were calculated for both, cell-by-cell and slide-by-slide classifications. Seven nuclear features showeddiscriminative ability between smokers and non-smokers; most of them were nuclear texture features. Statistical analysis of nuclear features in groups of females and males hoswed that only two nuclear features were different. It is concluded that smoking should be considered in image cytometry analysis of lesions in oral cavity. Objavljeno v DiRROS: 19.01.2024; Ogledov: 127; Prenosov: 38 Celotno besedilo (1,23 MB) |
14. Longitudinal study of malignancy associated changes in progressive cervical dysplasiaMargareta Strojan Fležar, Jaka Lavrenčak, Mario Žganec, Marija Us-Krašovec, 1998, izvirni znanstveni članek Povzetek: Eight of 29 patients with progressive CIN were followed for 2 to 10 years. Their consecutive Pap smears were destained and stained according to Feulgen thionin method. Cyto-Savant/TM high resolution image cytometer (Oncometrics Technol. Corp., Vancouver, Canaderage values of muclear texture features and their probability distributions for consecutive Pap smears from each patient were calculated. Three out of 5 discriminant MAC, highDNAamount, highDNAcomp and highDNAarea, were to increase as a function of time in 5 out of 8 patients. A preliminary analysis which was performed on non-standardized archival material demonstrated a monotonous increase of discrete texture features as a function of time in patients with progressive CIN. Objavljeno v DiRROS: 19.01.2024; Ogledov: 138; Prenosov: 34 Celotno besedilo (1,39 MB) |
15. Vinblastine increases antitumor effectiveness of bleomycinMaja Čemažar, Marija Auersperg, Gregor Serša, 1997, izvirni znanstveni članek Povzetek: In our previous vinblastine (VELBE) was shown to increase the plasma membrane fluidity. This effect of VELBE might be expolited for better transport of drugs through the plasma membrane. Bleomycin (BLM) is a highly cytotoxic drug when present inside the cells but has a hampered transport through the plasma membrane. The aim of the present study was to determine whether pretreatment with VELBE can increase the effect of BLM on intraperitoneal SA-1 tumors in mice. BLM and VELBE were used as single agents or in various combinations, i.e. VELBE and BLM injected simultanously, BLM injected 24 h before VELBE or VELBE injected 24 h before BLM. Mice survival was the end-point used for determining the effect of this combined treatments. VELBE and BLM as a single treatment significantly prolonged median survival time of study animals compared to controls. Furthermore, when VELBE and BLM were combined, all threetested combinations were more effective than VELBE or BLM as single treatments. The effect on animal survival was equal when VELBE was given 24 h after or simultanously with BLM. The longest survival, however was obtained when VELBE was injected 24 h before BLM. From these results we can conclude that the underlying mechanisms for more than additive effect of VELBE and BLM when VELBE was given 24 h before BLM could be attributed to an increased membrane fluidity, possibly in combination with a cell kinetic effect. Objavljeno v DiRROS: 18.01.2024; Ogledov: 114; Prenosov: 25 Celotno besedilo (1,28 MB) |
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18. Prognostic value of staging laparotomy in supradiaphragmatic clinical stage I and II Hodgkin's diseaseMarjeta Vovk, Tatjana Šumi-Križnik, Marija Jenko-Fidler, Gabrijela Petrič-Grabnar, Marinka Kremžar, Janez Novak, Mojca Senčar, Branko Zakotnik, Alenka Vodnik-Cerar, Branimir Jakšić, 1996, izvirni znanstveni članek Povzetek: In the period 1974-1989, 219 patients with supradiaphragmatic clinical stage I and II Hodgkin's disease were treated at the Institute of Oncology in Ljubljana; of these 95 (43%) patients underwent staging laparotomy. Of laparotomized patients, those with pathological stage III-IV, and of non-laparotomized, those with unfavorable prognostic factors (B-symptoms, bulky mediastinum) received chemotherapy: the remaining patients were treated by irradiation. No statistically significant difference in the survival and disease-free survival between laparotomized and nonlaparotomized patients could be found. Objavljeno v DiRROS: 16.01.2024; Ogledov: 149; Prenosov: 42 Celotno besedilo (364,35 KB) |
19. Biological behaviour of lung carcinoids : a retrospective analysis of 71 patientsIzidor Kern, Tomaž Rott, Marija Zupančič, Jurij Šorli, Urška Gantar-Rott, Janez Eržen, Janez Orel, Bogo Hrabar, 1994, strokovni članek Ključne besede: rak (medicina), pljuča, pljučni rak, novotvorbe Objavljeno v DiRROS: 11.01.2024; Ogledov: 149; Prenosov: 37 Celotno besedilo (478,69 KB) |
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