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Objavljeno v DiRROS: 28.04.2021; Ogledov: 873; Prenosov: 305
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Objavljeno v DiRROS: 26.03.2021; Ogledov: 1093; Prenosov: 346
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Ključne besede: bolnice, zdravstveni nasveti
Objavljeno v DiRROS: 15.03.2021; Ogledov: 1385; Prenosov: 337
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Povzetek: In recent decades, there have been an increasing number of outbreaks of Ips typographus in Europe. A large amount of sanitary felling has taken place, with significant economic and ecological consequences. In order to anticipate such large%scale outbreaks, an effective monitoring system should be set up. One important aspect of monitoring is deciding which pheromone to use. Therefore, we decided to test five different commercially available pheromone lures under different disturbance conditions: Pheroprax%, IT Ecolure Extra%, Ipstyp%, Ipsowit% and Typosan%. We investigated the ability of the pheromones to distinguish between disturbed and undisturbed locations, their cost%efficiency ratio, and side effects such as bycatch abundance and composition. We set 50 traps in two areas with sites that were disturbed and undisturbed by windstorms. We collected the catch from traps every 1%2 weeks from the end of March until the end of September in 2019. We found that IT Ecolure Extra%, Ipsowit% and % Pheroprax% caught the most I. typographus and best showed changes in the trap catch of I. typographus throughout the whole season. There was a low amount of bycatch (<6% of the total catch) and a low number of predators (a few specimens), but some groups seem to prefer certain pheromones. The cost of the pheromones increased with their effectiveness. However, pheromone costs are low relative to the personnel costs involved in setting traps and collecting the catch. Based on all of the gathered data, we created an index which helps to assess the cost%efficiency of the five chosen commercially available pheromones. We also present guidelines on how to make such an index to assist other researchers in choosing the right pheromone for monitoring populations of I. typographus or other bark beetle species.
Ključne besede: bark beetles, cost-efficiency, Ips typographus, monitoring pheromones, Picea abies
Objavljeno v DiRROS: 21.01.2021; Ogledov: 1412; Prenosov: 773
Celotno besedilo (1,21 MB)
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Povzetek: Mycobacterium abscessus complex (MABC) is a taxonomic group of rapidly growing, nontuberculous mycobacteria that are found as etiologic agents of various types of infections. They are considered as emerging human pathogens. MABC consists of 3 subspecies - M. abscessus subsp. bolletti, M. abscessus subsp. massiliense and M. abscessus subsp. abscessus. Here we present a novel method for subspecies differentiation of M. abscessus named Subspecies-Specific Sequence Detection (SSSD). This method is based on the presence of signature sequences present within the genomes of each subspecies of MABC. We tested this method against a virtual database of 1505 genome sequences of MABC. Further, we detected signature sequences of MABC in 45 microbiological samples through DNA hybridization. SSSD showed high levels of sensitivity and specificity for differentiation of subspecies of MABC, comparable to those obtained by rpoB sequence typing.
Ključne besede: Mycobacterium abscessus complex, nontuberculous mycobacteria, diagnosis
Objavljeno v DiRROS: 23.11.2020; Ogledov: 12031; Prenosov: 1000
Celotno besedilo (1,23 MB)
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