1. The Slovenian translational platform GlioBank for brain tumour research : identification of molecular signatures of glioblastoma progressionMetka Novak, Bernarda Majc, Marta Malavolta, Andrej Porčnik, Jernej Mlakar, Matjaž Hren, Anamarija Habič, Mateja Mlinar, Ivana Jovchevska, Neja Šamec, Alja Zottel, Marija Skoblar Vidmar, Tina Vipotnik-Vesnaver, Andrej Zupan, Alenka Matjašič, Saša Trkov, Dejan Georgiev, Aleksander Sadikov, Roman Bošnjak, Borut Prestor, Radovan Komel, Tamara Lah Turnšek, Barbara Breznik, 2025, izvirni znanstveni članek Ključne besede: biobank, biomarker, glioblastoma, tumour models, oncology Objavljeno v DiRROS: 28.01.2025; Ogledov: 252; Prenosov: 144
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3. Patient-derived tumor organoids mimic treatment-induced DNA damage response in glioblastomaBernarda Majc, Anamarija Habič, Marta Malavolta, Miloš Vittori, Andrej Porčnik, Roman Bošnjak, Jernej Mlakar, Alenka Matjašič, Andrej Zupan, Marija Skoblar Vidmar, Tamara Lah Turnšek, Aleksander Sadikov, Barbara Breznik, Metka Novak, 2024, izvirni znanstveni članek Povzetek: Glioblastoma (GB) is the most common primary malignant brain tumor, characterized by resistance to therapy. Despite aggressive treatment options, GB remains an incurable disease. Invasiveness and heterogeneity are key GB features that cannot be studied in preclinical in vitro models. In this study, we investigated the effects of standard therapy using patient-derived GB organoids (GBOs). GBOs reflect the complexity and heterogeneity of the original tumor tissue. No significant effect on GBO viability or invasion was observed after irradiation and temozolomide treatment. E3 ubiquitin-protein ligase (MDM2), cyclin-dependent kinase inhibitor 1A (CDKN1A), and the serine/threonine kinases ATM and ATR were upregulated at the gene and protein levels after treatment. Our results show that the p53 pathway and DNA-damage response mechanisms were triggered, suggesting that GBOs recapitulate GB therapy resistance. GBOs thus provide a highly efficient platform to assess the specific responses of GB patients to therapy and to further explore therapy resistance. Ključne besede: cellular physiology, cellular toxicology, in vitro toxicology including 3D culture, technical aspects of cell biology, cancer Objavljeno v DiRROS: 09.09.2024; Ogledov: 605; Prenosov: 255
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4. Cognitive functioning in a cohort of high-grade glioma patientsAndreja Cirila Škufca Smrdel, Anja Podlesek, Marija Skoblar Vidmar, Jana Markovič, Jana Jereb, Manja Kuzma, Uroš Smrdel, 2023, izvirni znanstveni članek Povzetek: High grade gliomas are associated with cognitive problems. The aim of the study was to investigate cognitive functioning in a cohort of patients with high grade glioma, according to isocitrate dehydrogenase (IDH) and methyl guanine methyl transferase (MGMT) status and other clinical characteristics. The patients with the high-grade glioma treated in Slovenia in given period of time were included in study. Postoperatively they completed neuropsychological assessment consisting of Slovenian Verbal Learning Test, Slovenian Controlled Oral Word Association Test, Trail Making Test Part A and B and self-evaluation questionnaire. We analysed results (z-scores and dichotomized results) also according to IDH mutation and MGMT methylation. We examined differences between groups using T-test, Mann-Whitney U, χ2 and Kendall's Tau tests. Out of 275 patients in the cohort, we included 90. Forty-six percent of patients were unable to participate due to poor performance status and other conditions related to tumour. Patients with the IDH mutation were younger, with better performance status, larger proportions of grade III tumours and MGMT methylation. In this group cognitive functioning is significantly better in the domains of immediate recall, short delayed recall and delayed recall, and in the fields of executive functioning and recognition. There were no differences in cognitive functioning in regard to MGMT status. Grade III tumours were associated with more frequent MGMT methylation. Self-assessment proved week tool, associated only with immediate recall. We found no differences in cognitive functioning according to MGMT status, but cognition was better when IDH mutation was present. In a cohort study of patients with high-grade glioma, almost half were unable to participate in a study, which points to an overrepresentation of patients with better cognitive functioning in the research. Ključne besede: cognition, high grade glioma, IDH1 mutation Objavljeno v DiRROS: 25.07.2024; Ogledov: 574; Prenosov: 187
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5. Definitive radiochemotherapy in esophageal cancer : a single institution experienceFranc Anderluh, Miha Toplak, Vaneja Velenik, Irena Oblak, Ajra Šečerov Ermenc, Ana Jeromen, Jasna But-Hadžić, Marija Skoblar Vidmar, 2019, izvirni znanstveni članek Ključne besede: radiochemotherapy, definitive radiochemotherapy, esophageal cancer Objavljeno v DiRROS: 05.07.2024; Ogledov: 612; Prenosov: 151
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7. Mesenteric ischemia after capecitabine treatment in rectal cancer and resultant short bowel syndrome is not an absolute contraindication for radical oncological treatmentAna Perpar, Erik Brecelj, Nada Rotovnik-Kozjek, Franc Anderluh, Irena Oblak, Marija Skoblar Vidmar, Vaneja Velenik, 2015, drugi znanstveni članki Povzetek: Thrombotic events, arterial or venous in origin, still remain a source of substantial morbidity and mortality in cancer patients. The propensity for their development in oncology patients is partially a consequence of the disease itself and partially a result of our attempts to treat it. One of the rarest and deadliest thromboembolic complications is arterial mesenteric ischemia. The high mortality rate is caused by its rarity and by its non-specific clinical presentation, both of which make early diagnosis and treatment difficult. Hence, most diagnoses and treatments occur late in the course of the disease. The issue survivors of arterial mesenteric ischemia may face is short bowel syndrome, which has become a chronic condition after the introduction of parenteral nutrition at home. We present a 73-year-old rectal cancer patient who developed acute arterial mesenteric thrombosis at the beginning of the pre-operative radiochemotherapy. Almost the entire length of his small intestine, except for the proximal 50 cm of it, and the ascending colon had to be resected. After multiorgan failure his condition improved, and he was able to successfully complete radical treatment (preoperative radiotherapy and surgery) for the rectal carcinoma, despite developing short bowel syndrome (SBS) and being dependent upon home-based parenteral nutrition to fully cover his nutritional needs. Mesenteric ischemia and resultant short bowel syndrome are not absolute contraindications for radical oncological treatment since such patients can still achieve long-term remission. Ključne besede: acute mesenteric ischemia, capecitabine, multiorgan failure, rectal cancer, short bowel syndrome Objavljeno v DiRROS: 23.04.2024; Ogledov: 769; Prenosov: 201
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8. Capecitabine in adjuvant radiochemotherapy for gastric adenocarcinomaIrena Oblak, Marija Skoblar Vidmar, Franc Anderluh, Vaneja Velenik, Ana Jeromen, Jasna But-Hadžić, 2014, izvirni znanstveni članek Povzetek: Background. In patients with non-metastatic gastric cancer surgery still remains the treatment of choice. Postoperative radiochemotherapy with 5-fluorouracil and leucovorin significantly improves the treatment outcome. The oral fluoropyrimidines, such as capecitabine, mimic continuous 5-fluorouracil infusion, are at least as effective as 5-fluorouracil, and such treatment is more comfortable for the patients. Patients and methods. In the period from October 2006 to December 2009, 101 patients with gastric cancer in stages Ib-IIIc were treated with postoperative chemoradiation with capecitabine. Distal subtotal resection of the stomach was performed in 46.3%, total resection in 50.5% and multivisceral resection in 3.2% of patients. The main endpoints of this study were loco-regional control (LRC), disease-free survival (DFS), disease-specific survival (DSS) and overall survival (OS). The rates of acute side-effects were also estimated. Results. Seventy-seven percent of patients completed the treatment according to the protocol. The median followup time of all patients was 3.9 years (range: 0.4-6.3 years) and in survivors it was 4.7 years (range: 3.2-6.3 years). No death occurred due to the therapy. Acute toxicity, such as nausea and vomiting, stomatitis, diarrhoea, hand-foot syndrome and infections of grade 3 or 4, occurred in 5%, 1%, 2%, 8.9% and 18.8% of patients, respectively. On the close-out date 63.4% patients were still alive and with no signs of the disease. The 4-years follow-up survey showed that LRC, DFS, DSS and OS were 95.5%, 69.2%, 70.7%, and 66.2%, respectively. Higher pN-stage and splenectomy were found to be independent prognostic factors for all four types of survival and perineural invasion and lower treatment intensity for DFS, DSS and OS. Ključne besede: gastric cancer, adjuvant therapy, radiochemotherapy Objavljeno v DiRROS: 11.04.2024; Ogledov: 744; Prenosov: 237
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9. Results of postoperative radiochemotherapy of the patients with resectable gastroesophageal junction adenocarcinoma in SloveniaAna Jeromen, Irena Oblak, Franc Anderluh, Vaneja Velenik, Marija Skoblar Vidmar, Ivica Ratoša, 2012, izvirni znanstveni članek Ključne besede: gastroesophageal junction adenocarcinoma, postoperative radiochemotherapy, toxicity Objavljeno v DiRROS: 21.03.2024; Ogledov: 752; Prenosov: 227
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10. Tehnologija Hyper Arc za izvedbo stereotaktične radiokirurgije v zdravljenju možganskih metastazMarija Skoblar Vidmar, 2023, drugi znanstveni članki Povzetek: Hyper Arc je inovativna programska in strojna oprema za izvedbo stereotaktične radiokirurgije, ki je od junija 2020 dostopna na Onkološkem inštitutu Ljubljana. Omogoča visoko natančno, monoizocentrično obsevanje v teoriji neomejenega števila možganskih tarč. Najbolj uporabna je v lokalnem zdravljenju možganskih metastaz, ki so vedno pogostejši vzrok obolevnosti in umrljivosti bolnikov z malignimi boleznimi.Glede na najnovejše raziskave in smernice se stereotaktična radiokirurgija obravnava kot primarna izbira za izboljšanje preživetja in je lahko najboljša izbira zdravljenja več metastaz v možganih. Lokalna kontrola vsake obsevane lezije in morebitna pozna toksičnost sta vedno pomembnejšega kliničnega pomena, ki lahko vplivata ne le na preživetje, temveč tudi na nevrokognitivno funkcijo in kakovost življenja. Ključne besede: Hyper Arc, stereotaktična radiokirurgija, možganske metastaze Objavljeno v DiRROS: 27.07.2023; Ogledov: 1044; Prenosov: 263
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