Digitalni repozitorij raziskovalnih organizacij Slovenije

Iskanje po repozitoriju
A+ | A- | Pomoč | SLO | ENG

Iskalni niz: išči po
išči po
išči po
išči po

Možnosti:
  Ponastavi


Iskalni niz: "avtor" (Margareta Strojan Fležar) .

1 - 10 / 45
Na začetekNa prejšnjo stran12345Na naslednjo stranNa konec
1.
Microinvasive cervical squamous cell carcinoma in Slovenia during the period 2001-2007
Helena Gutnik, Jasenka P. Matišić, Maja Primic-Žakelj, Margareta Strojan Fležar, 2014, izvirni znanstveni članek

Povzetek: Background. Microinvasive squamous cell carcinoma (MISCC) comprises a significant portion of all cervical cancers in Slovenia. Criteria of carcinomatous invasion are well described in the literature, however histopathological assessment of MISCC is difficult, because morphological characteristics can overlap with cervical intraepithelial neoplasia grade 3 (CIN 3) and other pathological changes. The aim of our study was to evaluate the reliability of the histopathological diagnosis of MISCC in Slovenia during the period from 2001 to 2007. Materials and methods. Data on patients with a histopathological diagnosis of cervical MISCC (FIGO stage IA) in the period of 2001 to 2007 were obtained from the Cancer Registry of Slovenia. Histological slides were obtained from the majority of pathology laboratories in Slovenia. We received 250 cases (69% of all MISCC) for the review; 30 control cases with CIN 3 and invasive squamous cell carcinoma FIGO stage IB were intermixed. The slides were coded and reviewed. Results. Among 250 cases originally diagnosed as MISCC, there was an agreement with MISCC diagnosis in 184 (73.6%) cases (of these 179/184 (97.3%) cases were FIGO stage IA1 and 5/184 (2.7%) cases were FIGO stage IA2). Among 179 FIGO stage IA1 cases 117 (65.4%) showed only early stromal invasion. Conclusions. The retrospective review of cases diagnosed as MISCC during the period 2001- 2007 in Slovenia showed a considerable number of overdiagnosed cases. Amongst cases with MISCC confirmed on review, there was a significant proportion with early stromal invasion (depth of invasion less than 1 mm).
Ključne besede: cervical cancer, cervical squamous cell carcinoma, microinvasive squamous cell carcinoma, intraepithelial neoplasia
Objavljeno v DiRROS: 11.04.2024; Ogledov: 479; Prenosov: 302
.pdf Celotno besedilo (969,61 KB)

2.
Image cytometric nuclear texture features in inoperable head and neck cancer : a pilot study
Margareta Strojan Fležar, Jaka Lavrenčak, Mario Žganec, Primož Strojan, 2011, izvirni znanstveni članek

Povzetek: Background. Image cytometry can measure numerous nuclear features which could be considered a surrogate end-point marker of molecular genetic changes in a nucleus. The aim of the study was to analyze image cytometric nuclear featuresin paired samples of primary tumor and neck metastasis in patients with inoperable carcinoma of the head and neck. Materials and methods. Image cytometric analysis of cell suspensions prepared from primary tumor tissue andfine needle aspiration biopsy cell samples of neck metastases from 21 patients treated with concomitant radiochemotherapy was performed. Nuclear features were correlated with clinical characteristics and response to therapy. Results. Manifestation of distant metastases and new primaries was associated (p<0.05) with several chromatin characteristics from primary tumor cells, whereas the origin of index cancer and disease response in the neck wasrelated to those in the cells from metastases. Many nuclear features of primary tumors and metastases correlated with the TNM stage. Conclusions. A specific pattern of correlation between well-established prognostic indicatorsand nuclear features of samples from primary tumors and those from neck metastases was observed. Image cytometric nuclear features represent a promising candidate marker for recognition of biologically different tumor subgroups.
Objavljeno v DiRROS: 19.03.2024; Ogledov: 223; Prenosov: 68
.pdf Celotno besedilo (427,02 KB)

3.
Urine and bladder washing cytology for detection of urothelial carcinoma : standard test with new possibilities
Margareta Strojan Fležar, 2010, pregledni znanstveni članek

Povzetek: Background. Light microscopic evaluation of cell morphology in preparations from urine or bladder washing containing exfoliated cells is a standard and primary method for the detection of bladder cancer and also malignancy from other parts of the urinary tract. The cytopathologic examination is a valuablemethod to detect an early recurrence of malignancy or new primary carcinoma during the follow-up of patients after the treatment of bladder cancer. Conclusions. Characteristic cellular and nuclear signs of malignancy indicate invasive or in situ urothelial carcinoma or high-grade papillary urothelial carcinoma. However, low sensitivity of the method reflects the unreliable cytopathologic diagnosis of low-grade urothelial neoplasms as cellular and nuclear signs of malignancy in these neoplasms are poorly manifested. Many different markers were developed to improve the diagnosis of bladder carcinoma on urinary samples. UroVysionT test is among the newest and most promising tests. By the method of in situ hybridization one can detect specific cytogenetic changes of urothelial carcinoma.
Ključne besede: rak (medicina), diagnostika, citološka diagnostika, sečni mehur
Objavljeno v DiRROS: 18.03.2024; Ogledov: 194; Prenosov: 64
.pdf Celotno besedilo (589,07 KB)

4.
Image cytometry analysis of normal buccal mucosa smears: influence of smoking and sex-related differences
Jaka Lavrenčak, Margareta Strojan Fležar, Mario Žganec, Marija Us-Krašovec, 1998, izvirni znanstveni članek

Povzetek: To get more information about the influence of smoking on chromatin pattern ofnormal buccal mucosa cells and to assess sex-related differences in nuclear features, quantitative analysis of normal buccal mucosa smears was performed. In this study, buccal smears were collected from 78 healthy subjects. Image cytometry analysis was performed on Feulgen-thionin stained smears. probability distributions of 78 nuclear features were calculated for both, cell-by-cell and slide-by-slide classifications. Seven nuclear features showeddiscriminative ability between smokers and non-smokers; most of them were nuclear texture features. Statistical analysis of nuclear features in groups of females and males hoswed that only two nuclear features were different. It is concluded that smoking should be considered in image cytometry analysis of lesions in oral cavity.
Objavljeno v DiRROS: 19.01.2024; Ogledov: 268; Prenosov: 84
.pdf Celotno besedilo (1,23 MB)

5.
Longitudinal study of malignancy associated changes in progressive cervical dysplasia
Margareta Strojan Fležar, Jaka Lavrenčak, Mario Žganec, Marija Us-Krašovec, 1998, izvirni znanstveni članek

Povzetek: Eight of 29 patients with progressive CIN were followed for 2 to 10 years. Their consecutive Pap smears were destained and stained according to Feulgen thionin method. Cyto-Savant/TM high resolution image cytometer (Oncometrics Technol. Corp., Vancouver, Canaderage values of muclear texture features and their probability distributions for consecutive Pap smears from each patient were calculated. Three out of 5 discriminant MAC, highDNAamount, highDNAcomp and highDNAarea, were to increase as a function of time in 5 out of 8 patients. A preliminary analysis which was performed on non-standardized archival material demonstrated a monotonous increase of discrete texture features as a function of time in patients with progressive CIN.
Objavljeno v DiRROS: 19.01.2024; Ogledov: 280; Prenosov: 77
.pdf Celotno besedilo (1,39 MB)

6.
Citodiagnostika netipljivih lezij v dojkah
Ana Pogačnik, Margareta Strojan Fležar, 2004, objavljeni znanstveni prispevek na konferenci

Povzetek: Izhodišča. Aspiracijska biopsija s tanko iglo (ABTI) je uveljavljena metoda zadiagnostiko sprememb v dojki. V pričujoči študiji smo želeli ugotoviti zanesljivost ultrazvočno (UZ) ali rentgensko (RTG) vodene ABTI za diagnostiko netipljivih lezij v dojki na podlagi cito-histološke korelacije in izračunati občutljivost, specifičnost, napovedno veljavnost pozitivne in negativne diagnoze. Bolniki in metode. V retrospektivno študijo smo vključili 179 žensk,pri katerih je bila v letu 2003 izvršena UZ ali/in RTG vodena ABTI netipljive lezije v dojki, ki je bila kasneje histološko opredeljena na vzorcubiopsije z debelo iglo, vodeni ekscizijski biopsiji ali na kirurškem resektatu. Rezultati. V 66 (36,8%) primerih je bil histološko dokazan karcinom, v 113 (63,1%) primerih pa benigna lezija. Citopatološke diagnoze v skupini karcinomov so bile: karcinom v 41 primerih (62,1%), sumljivo za karcinom v 12 (18,2%), napačno negativna diagnoza v 3 (4,5%), v 10 (15%) primerih pa niso dobili dovolj materiala za diagnozo. Zaključki. V skupini benignih lezij smo iz vzorca ABTI postavili pravilno negativno diagnozo v 55 primerih (48,7%), napačno sumljivo v 18 (15,9%), napačno pozitivno diagnozo v enem primeru (0,88%) in v 39 (34,5%) primerih nismo dobili dovolj materiala zadiagnozo. Občutljivost metode v naši študiji je bila 91%, specifičnost 98%, napovedna veljavnost pozitivne diagnoze 97,6% in napovedna veljavnost negativne diagnoze 94,8 %.
Objavljeno v DiRROS: 01.12.2023; Ogledov: 281; Prenosov: 94
.pdf Celotno besedilo (323,74 KB)

7.
8.
Navodila za citološke izvide brisov materničnega vratu : klasifikacija po Bethesdi
Ana Pogačnik, Margareta Strojan Fležar, Alenka Repše-Fokter, Vivijana Snoj, Irena Srebotnik-Kirbiš, Maja Primic-Žakelj, 2020, slovar, enciklopedija, leksikon, priročnik, atlas, zemljevid

Povzetek: V Sloveniji poteka državni presejalni program za zgodnje odkrivanje predrakavih sprememb (ZORA) materničnega vratu od leta 2003. Od leta 2003 do 2008 smo znižali obolevnost za rakom materničnega vratu za skoraj 40 %, vendar pa uspešnost programa lahko še izboljšamo, tudi z rednim preverjanjem kakovosti dela ginekologov in citoloških laboratorijev, saj je spremljanje in nadziranje kakovosti vseh postopkov bistveni sestavni del vseh organiziranih presejalnih programov. Register ZORA zbira izvide brisov materničnega vratu (BMV) iz vseh desetih slovenskih laboratorijev, ki ustrezajo pogojem, določenim v Uradnem listu RS. Z registracijo vseh izvidov BMV smo vzpostavili učinkovit sistem spremljanja kakovosti vseh presejalnih postopkov.
Ključne besede: bris, citopatologija, klasifikacija, terminologija, elektronske knjige
Objavljeno v DiRROS: 10.03.2022; Ogledov: 923; Prenosov: 414
.pdf Celotno besedilo (3,83 MB)
Gradivo ima več datotek! Več...

9.
10.
Delovanje citopatoloških in histopatoloških laboratorijev v času pandemije COVID-19 s prikazom primera
Margareta Strojan Fležar, 2020, objavljeni znanstveni prispevek na konferenci

Ključne besede: citopatologija, histopatologija, predrakave spremembe
Objavljeno v DiRROS: 17.05.2021; Ogledov: 905; Prenosov: 499
.pdf Celotno besedilo (3,08 MB)
Gradivo ima več datotek! Več...

Iskanje izvedeno v 0.35 sek.
Na vrh