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1.
Data for: Unveiling microalgal diversity in Slovenian transitional waters (Adriatic Sea): a first step toward ecological status assessment : version v1
Petra Slavinec, Janja Francé, Ana Fortič, Patricija Mozetič, 2026, zaključena znanstvena zbirka raziskovalnih podatkov

Povzetek: List of microalgal taxa recorded in Slovenian transitional waters (coastal brackish lagoon, estuary) and the coastal sea.
Ključne besede: diversity, microalgae, seaweed, coastal ecosystems, Gulf of Trieste
Objavljeno v DiRROS: 22.05.2026; Ogledov: 154; Prenosov: 155
.zip Raziskovalni podatki (27,28 KB)
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2.
Phytoplankton diversity in the Northern Adriatic Sea : insights and inconsistencies from microscopy and metabarcoding
Giorgia Montali, Francesca Neri, Elisa Banchi, Federica Cerino, Timotej Turk Dermastia, Janja Francé, Patricija Mozetič, 2026, izvirni znanstveni članek

Povzetek: Phytoplankton plays a fundamental role in marine ecosystems and is widely used to assess environmental change. In this study, light microscopy and DNA metabarcoding approaches were used to investigate phytoplankton communities at three long-term monitoring sites in the northern Adriatic Sea. By combining the two approaches, more than 500 species were recorded. Metabarcoding detected substantially higher diversity, particularly among small and fragile organisms that are difficult to identify under a microscope, whereas microscopy was better for identifying larger species with distinctive morphology. Because species contain different amounts of genetic material, DNA data can overestimate or underestimate their true contribution to the community. Indeed, after applying correction factors that account for these differences, the DNA-based estimates became more consistent with the microscopy results for several phytoplankton groups. The differences among the samples were influenced more by the approach used than by the sampling location. Overall, combining these two approaches provides an accurate and robust picture of phytoplankton communities. In this way, it is possible to improve the reliability of marine biodiversity assessments and to support better environmental monitoring and management in coastal seas.
Ključne besede: phytoplankton, diversity, environmental DNA, Long-Term Ecological Research, amplicon sequencing, North Adriatic Sea
Objavljeno v DiRROS: 25.03.2026; Ogledov: 289; Prenosov: 220
.pdf Celotno besedilo (1,67 MB)
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3.
Microalgal communities in mucilage aggregates (Northern Adriatic Sea, summer 2024) based on microscopy and metabarcoding
Marika Ubaldi, Francesca Neri, Giorgia Montali, Tiziana Romagnoli, Aurora Tomasini, Federica Cerino, Timotej Turk Dermastia, Patricija Mozetič, Janja Francé, Camilla Spoto, Stefano Accoroni, Cecilia Totti, 2026, izvirni znanstveni članek

Povzetek: The mucilage phenomenon consists of the appearance of large gelatinous aggregates floating in the water column. In summer 2024, this event has reappeared in the Northern Adriatic Sea (NAS) on a large scale. This study provides an integrated characterization of the microalgal community within mucilage aggregates and surrounding waters in two NAS areas (Gulf of Trieste and Conero Riviera) using light microscopy (LM), metabarcoding (MB) based on ribosomal 18S V4 and V9 markers, and scanning electron microscopy (SEM) for selected taxa identification. Mucilage aggregates acted as dynamic microbial hotspots, hosting a rich diatom community, with abundances 1–2 orders of magnitude higher than in the surrounding water. Dominant diatom species were Cylindrotheca closterium, Nitzschia spp., Nitzschia gobbii, and Thalassionema nitzschioides. Some phytoflagellates (e.g., Tetraselmis spp.) and dinoflagellates (e.g., Karlodinium veneficum, Pseliodinium fusus, and Wangodinium sinense) were detected exclusively by MB, while LM and SEM revealed species missed by other approaches. Gonyaulax fragilis, one of the species indicated as an important mucus producer, was present at the onset and throughout the phenomenon, as detected by LM and MB. The integrated approach improves knowledge of microalgal communities in NAS mucilage.
Ključne besede: mucilage, Northern Adriatic Sea, phytoplankton, microalgae, metabarcoding, microscopy, Gonyaulax fragilis, Harmful Algal Blooms
Objavljeno v DiRROS: 27.01.2026; Ogledov: 447; Prenosov: 356
.zip Celotno besedilo (483,26 KB)
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4.
Unveiling microalgal diversity in Slovenian transitional waters (Adriatic Sea) : a first step toward ecological status assessment
Petra Slavinec, Janja Francé, Ana Fortič, Patricija Mozetič, 2026, izvirni znanstveni članek

Povzetek: This study presents the first comprehensive assessment of microalgal diversity in two Slovenian transitional waters (TWs): the shallow brackish lagoon of the Škocjanski Zatok Nature Reserve (SZNR) and the Rižana River estuary within the Port of Koper (PK) area. Between 2018 and 2021, water samples collected with a phytoplankton net were analyzed using light and scanning electron microscopy. In total, 240 species from 117 genera were identified in TW, dominated by diatoms and dinoflagellates, surpassing the diversity at a marine coastal station (91 species, 59 genera). Species richness was higher in PK (226) than in SZNR (154), mainly due to dinoflagellates and coccolithophores. Marine taxa predominated along the salinity gradient, with moderate contributions from brackish taxa and fewfreshwater forms, reflecting both natural and anthropogenic influences. Planktonic taxa dominated at all sites, while benthic forms were abundant in the lagoon, particularly in spring. Thirty-two taxa were recorded for the first time in Slovenian TW, mostly benthic or tychopelagic diatoms. The detection of Coolia monotis and five cyanobacterial genera with potentially harmful traits highlights the role of TW as an ecological interface. The taxonomic sufficiency analysis showed that the order level is sufficient to distinguish transitional from marine assemblages, beyond which ecological information is lost. Overall, this study highlights the importance of detailed taxonomic resolution for detecting microalgal diversity, including harmful and non-indigenous species to ensure robust ecological assessments under the WFD and MSFD directives.
Ključne besede: transitional waters, diversity, phytoplankton, harmful algal blooms, non-indigenous species, Adriatic Sea
Objavljeno v DiRROS: 27.01.2026; Ogledov: 462; Prenosov: 443
.zip Celotno besedilo (3,43 MB)
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5.
Development and field testing of a cavitation-based robotic platform for sustainable in-water hull cleaning
Uroš Puc, Andreja Abina, Edvin Salvi, Vlado Malačič, Janja Francé, Riccardo Zanelli, Aleksander Zidanšek, 2026, izvirni znanstveni članek

Povzetek: Biofouling on ship hulls significantly increases hydrodynamic drag, fuel consumption, and greenhouse gas emissions, while also facilitating the spread of invasive species in regional and global waters, thereby threatening marine biodiversity. To address these environmental and economic issues, we developed an innovative robotic platform for in-water hull cleaning. The platform utilizes a cavitation-based cleaning module that removes biofouling while minimizing hull surface damage and preventing the spread of detached particles into the marine environment. This paper describes the design, operation, and testing of a developed robotic cleaning system prototype. Emphasis is placed on integrating components and sensors for continuous monitoring of key seawater parameters (temperature, salinity, turbidity, dissolved oxygen, chlorophyll-a, etc.) before, during, and after underwater cleaning. Results from real-sea trials show the platform’s effectiveness in removing biofouling and its minimal environmental impact, confirming its potential as a sustainable solution for in-water hull cleaning.
Ključne besede: biofouling, ship hulls, in-water cleaning, undewater robot, sensor integration, field testing
Objavljeno v DiRROS: 27.01.2026; Ogledov: 398; Prenosov: 528
.pdf Celotno besedilo (2,38 MB)
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