1. Truffle mycorrhization in a pecan orchardMylla Trisha Mello Souza, Joice Aline Freiberg, Tine Grebenc, Marcelo Aloisio Sulzbacher, Diniz Fronza, Juliano Borela Magalhães, Vanessa da Rosa Gonçalves, Nathalie Caroline Hirt Kessler, Lucas Moura Somavilla, Zaida Ines Antoniolli, 2024, izvirni znanstveni članek Povzetek: The ectomycorrhizal association of Tuber spp. in orchards is essential for truffle production. In this sense, this study aimed to evaluate the ectomycorrhizal association of Tuber aestivum Vittad. and Tuber brumale Vittad. in pecan after five years of cultivation in an orchard. The experiment was conducted in Santa Maria – Rio Grande do Sul, Brazil, in a pecan orchard planted with seedlings inoculated with these two species of truffles. The orchard was characterized in terms of soil physical and chemical properties and soil type. Initially non-inoculated plants, plants inoculated with Tuber aestivum (TA), and plants inoculated with Tuber brumale (TB) were evaluated for the percentage (%) of roots colonized by Tuber spp., other ectomycorrhizae, and non-mycorrhizal roots. A reduction in the colonization of both truffle species was observed five years after transplanting the seedlings to the orchard but they have the potential to maintain the ectomycorrhizal association with pecan seedlings.
Ključne besede: Carya illinoinensis, ectomycorrhiza, truffle, tuber
Objavljeno v DiRROS: 26.03.2024; Ogledov: 93; Prenosov: 55
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4. Designing innovative business models for the wild food products sector in several Mediterranean countriesAnže Japelj, Marta Rovira, Miriam Piqué, José Antonio Bonet, Daniel Oliach, Enrico Vidale, Nicola Andrighetto, José Guilherme Borges, Inês Conceição, Ibtissem Taghouti, Mariem Khalfaoui, Kaja Plevnik, Tine Grebenc, 2023, objavljeni znanstveni prispevek na konferenci Ključne besede: wild food products, Mediterranean, business model, innovation, living labs
Objavljeno v DiRROS: 06.10.2023; Ogledov: 326; Prenosov: 121
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6. Ectomycorrhiza between Scleroderma Areolatum Ehrenb. and Fagus sylvatica L.Tanja Mrak, Katja Kühdorf, Tine Grebenc, Ines Štraus, Marko Bajc, Nada Žnidaršič, Babette Münzenberger, Hojka Kraigher, 2015, objavljeni povzetek znanstvenega prispevka na konferenci Objavljeno v DiRROS: 03.11.2021; Ogledov: 761; Prenosov: 260
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7. Validation and verification of examination procedures in medical laboratories : opinion of the EFLM Working Group Accreditation and ISO/CEN standards (WG-A/ISO) on dealing with ISO 15189:2012 demands for method verification and validationRoseri Roelofsen-de Beer, Jos Wielders, Guilaine Boursier, Tatjana Vodnik, Florent Vanstapel, Wim Huisman, Ines Vukasović, Michel Vaubourdolle, Çiğdem Sönmez, Solveig Linko, Pika Meško-Brguljan, 2020, izvirni znanstveni članek Povzetek: This paper reflects the opinion of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group Accreditation and ISO/CEN standards (WG-A/ISO). It aims to provide guidance for drawing up local/national documents about validation and verification of laboratory methods. We demonstrate how risk evaluation can be used to optimize laboratory policies to meet intended use requirements as well as requirements of standards. This is translated in a number of recommendations on how to introduce risk evaluation in various stages of the implementation of new methods ultimately covering the whole process cycle.
Ključne besede: opinion paper, EFLM, ISO 15189:2012, validation, verification
Objavljeno v DiRROS: 10.08.2020; Ogledov: 1422; Prenosov: 417
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8. Different belowground responses to elevated ozone and soil water deficit in three European oak species (Quercus ilex, Q. pubescens and Q. robur)Tanja Mrak, Ines Štraus, Tine Grebenc, Jožica Gričar, Yasutomo Hoshika, Giulia Carriero, Elena Paoletti, Hojka Kraigher, 2019, izvirni znanstveni članek Povzetek: Effects on roots due to ozone and/or soil water deficit often occur through diminished belowground allocation of carbon. Responses of root biomass, morphology, anatomy and ectomycorrhizal communities were investigated in seedlings of three oak species: Quercus ilex L., Q. pubescens Willd. and Q. robur L., exposed to combined effects of elevated ozone (ambient air and 1.4 x ambient air) and water deficit (100% and 10% irrigation relative to field capacity) for one growing season at a free-air ozone exposure facility. Effects on root biomass were observed as general reduction in coarse root biomass by -26.8 % and in fine root biomass by -13.1 % due to water deficit. Effect on coarse root biomass was the most prominent in Q. robur (-36.3 %). Root morphological changes manifested as changes in proportions of fine root (<2 mm) diameter classes due to ozone and water deficit in Q. pubescens and due to water deficit in Q. robur. In addition, reduced fine root diameter (-8.49 %) in Q. robur was observed under water deficit. Changes in root anatomy were observed as increased vessel density (+18.5 %) due to ozone in all three species, as reduced vessel tangential diameter (-46.7 %) in Q. ilex due to interaction of ozone and water, and as generally increased bark to secondary xylem ratio (+47.0 %) due to interaction of ozone and water. Water deficit influenced occurrence of distinct growth ring boundaries in roots of Q. ilex and Q. robur. It shifted the ectomycorrhizal community towards dominance of stress-resistant species, with reduced relative abundance of Tomentella sp. 2 and increased relative abundances of Sphaerosporella brunnea and Thelephora sp. Our results provide evidence that expression of stress effects varies between root traits; therefore the combined analysis of root traits is necessary to obtain a complete picture of belowground responses.
Ključne besede: ozone, drought, fine roots, ectomycorrhiza, anatomy, morphology, plants
Objavljeno v DiRROS: 20.02.2020; Ogledov: 1985; Prenosov: 1298
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9. Fungos ectomicorrízicos em plantações de nogueira-pecã e o potencial da truficultura no BrasilMarcelo Aloisio Sulzbacher, Jonas Janner Hamann, Diniz Fronza, Rodrigo Josemar Seminoti Jacques, Admir Jose Giachini, Tine Grebenc, Zaida Ines Antoniolli, 2019, izvirni znanstveni članek Povzetek: Os estudos envolvendo a nogueira-pecã têm evoluído consideravelmente nos últimos anos no Brasil. Esta cultura foi introduzida no país no início do século passado e tornou-se importante comercialmente após os anos de 1960, principalmente na região sul. A nogueira-pecã é comprovadamente um simbionte, formando diversas associações mutualísticas com fungos ectomicorrízicos do solo. Assim, a presente revisão tem como objetivos apresentar os estudos sobre os fungos ectomicorrízicos em plantações comerciais de nogueirapecã no Brasil, destacar a importância agrícola e ambiental desta simbiose, e o potencial da micorrização controlada da nogueira-pecã, visando à produção de espécies desejadas de trufas, notadamente do gênero Tuber, em pomares comerciais, uma atividade econômica de alta rentabilidade.
Objavljeno v DiRROS: 25.07.2019; Ogledov: 1879; Prenosov: 823
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10. Tipi ektomikorize pri sadikah bukve (Fagus sylvatica L.) v rizotronihInes Štraus, Marko Bajc, Tine Grebenc, Boštjan Mali, Hojka Kraigher, 2011, izvirni znanstveni članek Povzetek: Naravni procesi ali dejavnost človeka vplivajo na razmere v okolju, kar se zrcali v sestavi ektomikoriznih združb. V raziskavi smo želeli ugotavljati morebitne vplive več temperaturnih režimov zraka in tal (substrata) na pojavljanje in vrstno pestrost tipov ektomikorize na koreninskih vršičkih sadik bukve in v substratu. V ta namen smo analizirali pojavljanje tipov ektomikorize na sadikah bukve v rizotronih, izpostavljenih štirim temperaturnim razmeram v okolju: 1.) zrak 15-25 °C; 2.) zrak 15-25 °C v kombinaciji s hlajenim koreninskim sistemom za 5 °C; 3.) povišana temperatura zraka od 30-50 °C in 4.) zunanja (ambientalna) temperatura zraka v Ljubljani. Tipe ektomikorize smo identificirali s kombiniranim pristopom po anatomsko morfoloških znakih in z analizo molekularnih markerjev (ITS1-5.8S rDNK-ITS2 ribosomalna regija v rDNK). Na 51 koreninskih vršičkih 40 sadik smo identificirali 6 tipov ektomikorize. Pestrost ektomikoriznih tipov je bila največja pri razmerah blizu optimalnih za rast bukve. Po vrstni sestavi združbe ektomikoriznih gliv sta si najbolj podobna poskusa s temperaturo zraka15-25 °C z ali brez hlajenja koreninskega sistema. Najpogostejša ektomikorizna vrsta je bila Hebeloma sacchariolens, ki je bila tudi najpogosteje opažena vrsta na drobnih koreninah odmrlih sadik. Izbrane vrste ektomikoriznih gliv smo z metodo DGGE dokazali tudi v vseh analiziranih vzorcih substrata.
Ključne besede: sadike bukve, ektomikoriza, temperatura zraka, temperatura substrata, molekularni markerji, filogenija
Objavljeno v DiRROS: 12.07.2017; Ogledov: 4816; Prenosov: 1976
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