1. Foliar application of protein hydrolysates promotes growth and affects leaf ionome in oliveIgor Pasković, Maša Andlovic, Helena Plešnik, Primož Vavpetič, Paula Žurga, Martin Šala, Marija Polić Pasković, Tina Kosjek, Paula Pongrac, 2026, izvirni znanstveni članek Povzetek: The foliar application of various biostimulants, such as protein hydrolysates (PHs), has been associated with improved nutrient uptake efficiency and stress tolerance in perennial crops, like olive (Olea europaea L.). In this study, PHs obtained by enzymatic hydrolysis by Alcalase Pure (referred to as treatment H1), Alcalase Pure and Flavourzyme (referred to as treatment H2), or Alcalase Pure and Protana™ Prime (referred to as treatment H3) with proteins from pumpkin seed cake were tested for their potential beneficial growth, performance, and nutrition effects in one-year-old olive seedlings grown under controlled conditions. Amino acid and element compositions were evaluated in the PHs, which were used for foliar application six times at eight-day intervals. Control (C) plants were treated the same way, but without PHs. Shoot and root growth, leaf reflectance indices, and the composition of micro and macronutrients in different organs and leaf tissues were determined. Plants in the H2 treatment grew significantly better than C plants. They had the highest Photochemical Reflectance Index and a Chlorophyll-Normalized Difference Vegetation Index similar to that of C plants, indicating an optimal growth/photosynthesis balance. A decrease in the concentration of several mineral elements in the lower epidermis in H2- and H3-treated plants compared to C and H1-treated plants was accompanied by their increase in the spongy mesophyll, indicating their redistribution to support increased metabolism, resulting in increased shoot growth in these two treatments. Arguably, these observed effects could be attributed to the amino acid profile of the H2 mixture, which had the highest concentration of L-proline, L-arginine, and L-lysine among the three PH mixtures, and a higher L-asparagine concentration than the H1 mixture. Overall, the results highlight the applicative potential of tailored PH formulations for the optimization of growth, mineral element composition, and physiological performance in olive cultivation.
Ključne besede: hydrolyzed proteins, pumpkin cake, biostimulants, element localization, olive cultivation, olive leaves
Objavljeno v DiRROS: 05.02.2026; Ogledov: 492; Prenosov: 126
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2. Nontargeted urinary profiling strategy for endocrine-disrupting chemicals in women with ovarian malignanciesHelena Plešnik, Žan Rekar, Stefanela Stevanović, Irma Virant-Klun, Senka Imamović-Kumalić, Mateja Sladič, Darja Mazej, Janja Snoj Tratnik, Milena Horvat, Tina Kosjek, 2025, izvirni znanstveni članek Povzetek: Endocrine-disrupting chemicals (EDCs), includingknown and unknown parent compounds, their metabolites, andtransformation products, are pervasive in daily life, posingincreasing risks to human health and the environment. Thisstudy employed a high-resolution mass spectrometry-basednontargeted screening approach, integrating polar (HILIC) andreversed-phase separations to expand the chemical space coverageand, supported by open-science tools and resources, evaluatedurinary chemical profiles to assess internal EDC exposure. Among106 annotated biomarkers of exposure, six exhibited significantlyhigher normalized intensities in patients with ovarian malignanciescompared to healthy controls (p < 0.05). This suggests theirgreater exposure to phthalates (diethylhexyl phthalate and diethylphthalate), pesticides (metolachlor metabolite and 4-nitrophenol), a UV filter (benzophenone-1), and an industrial byproduct (4-methyl-2-nitrophenol). These compounds may interfere with hormonal regulation, potentially contributing to cancer development.While these findings highlight potential differences in internal EDC exposure, the study primarily demonstrates the applicability ofnontargeted urinary profiling for chemical exposure assessment. By providing new insights into EDCs burden and its pathologicalimplications, this work contributes to advancing next-generation chemical risk assessment within the European Partnership for theAssessment of Risks from Chemicals initiative and supports the development of preventive strategies to mitigate environmentalcancer risks
Ključne besede: ovarian cancer, biomarkers, exposure, nontargeted screening, high-resolution mass spectrometry, analytical coverage, reversed-phase, HILIC
Objavljeno v DiRROS: 02.12.2025; Ogledov: 451; Prenosov: 170
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3. Bleomycin concentration in patientsʹ plasma and tumors after electrochemotherapy : a study from InspECT GroupAleš Grošelj, Maša Omerzel, Mojca Kržan, Tina Kosjek, Krisztina Bottyán, Helena Plešnik, Črt Jamšek, Maja Čemažar, Erika Kis, Gregor Serša, 2021, izvirni znanstveni članek Ključne besede: electrochemotherapy, electroporation, bleomycin
Objavljeno v DiRROS: 10.10.2022; Ogledov: 1830; Prenosov: 920
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