1. Chemokines during anaphylaxis : the importance of CCL2 and CCL2-dependent chemotactic activity for basophilsRomana Vantur, Maruša Rihar, Ana Koren, Matija Rijavec, Peter Kopač, Urška Bidovec, Renato Eržen, Peter Korošec, 2020 Abstract: Background: The role of chemokines in anaphylaxis is unclear. Methods: We prospectively recruited 49 patients presenting to the emergency department with an acute episode of anaphylaxis and 28 healthy subjects. We measured serum levels of the chemokines CCL2, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL21, CCL22, CCL24, and CCL26, tryptase, the absolute number of circulating basophils, monocytes, lymphocytes, and PMNs, and whole blood FCER1A, CPA3 and HDC gene expression at two time points: during the anaphylactic episode and in convalescent samples collected approximately 3 months later. We then investigated the in vitro chemotactic activity of chemokines induced during anaphylaxis for the in vitro migration of the corresponding cells. Results: Only CCL2 chemokine levels were signifcantly increased in anaphylaxis samples (median 514 pg/ml) compared to convalescent samples (284 pg/ml, P<0.0001) and healthy subjects (279 pg/ml, P<0.0001); there was no signifcant diference in any of the other chemokines. There was a signifcant positive correlation between the rates of increase of serum CCL2 (median [range]: 106.0% [-44.7% to 557.4%]) and tryptase (133.8% [-6.6% to 893.4%]; r=0.68, P<0.0001) and between the acute concentration of serum CCL2 and the acute concentration of serum tryptase (r=0.77, P<0.0001). The number of circulating basophils, but not other blood cells, signifcantly decreased during anaphylaxis (median 5.0 vs. 19.1 cells/[micro]l in convalescent samples; P<0.0001); a decrease in whole-blood gene expression of basophil markers (PKeywords: anaphylaxis, chemokines, tryptases, basophils, chemotaxis, CCL2, cell migration
DiRROS - Published: 18.01.2021; Views: 768; Downloads: 317
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2. Educational community and school systems : Slovenia : migrant children and communities in a transforming EuropeMateja Sedmak, Barbara Gornik, Zorana Medarić, Maja Zadel, Lucija Dežan, 2020 Keywords: migration, school system, educational system, schools, migrant learners, integration, Slovenia
DiRROS - Published: 10.05.2021; Views: 439; Downloads: 208
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4. Reflexive methodology : newly arrived migrant children, long-term migrant children, local children : SloveniaMateja Sedmak, 2021 Keywords: migration, school system, educational system, schools, migrant learners, surveys, interviews, participant observation, Slovenia
DiRROS - Published: 18.01.2022; Views: 247; Downloads: 87
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5. National report on qualitative research : newly arrived migrant children, long-term migrant children, local childrenMateja Sedmak, Lucija Dežan, 2021 Keywords: migration, school system, educational system, schools, migrant learners, integration, focus group, interviews, participant observation, Slovenia
DiRROS - Published: 18.01.2022; Views: 284; Downloads: 90
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