1. The impact of the covid−19 pandemic on nurses' and physicians' mental health : a literature reviewUroš Višić, Maja Sočan, 2022, review article Abstract: Introduction: The covid-19 pandemic is characterised by an increased risk of post-traumatic stress disorder in healthcare professionals. The purpose of this systematic review was to identify the impact of the covid-19 pandemic on the mental health of healthcare professionals. Methods: A systematic review of the literature was conducted. The literature search took place from November 2020 to January 2021. The search was conducted in the Pubmed, Ebsco, Ovid and Google Scholar databases, using the following combinations of keywords: self-image, fear, pandemic, prevention, nurses, physicians. The relevant records were selected on the basis of inclusion and exclusion criteria. The course of the literature review is shown using the PRISMA diagram. A thematic analysis was conducted with open coding of the results. Results: From the total search set, 14 articles were selected for final analysis. The prevalence of anxiety among nurses and physicians ranged from 12.3 to 35.6%. The prevalence of anxiety was higher in nurses, with the symptoms of anxiety being significantly lower in male nurses than in their female colleagues. Discussion and conclusion: The pandemic has left a significant psychological burden on nurses and physicians. There is therefore an urgent need to include preventive psychoeducational measures such as "briefing" and "teambuilding" in supervising mental health of nurses and physicians. Keywords: mental health, covid-19, health impact, risk factors, self-image, fear, pandemia, prevention, physicians, nurses Published in DiRROS: 28.01.2026; Views: 176; Downloads: 100
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2. Impact of indoor air pollution on DNA damage and chromosome stability : a systematic reviewLuka Kazensky, Katarina Matković, Marko Gerić, Bojana Žegura, Gordana Pehnec, Goran Gajski, 2024, review article Abstract: Indoor air pollution is becoming a rising public health problem and is largely resulting from the burning of solid fuels and heating in households. Burning these fuels produces harmful compounds, such as particulate matter regarded as a major health risk, particularly affecting the onset and exacerbation of respiratory diseases. As exposure to polluted indoor air can cause DNA damage including DNA sd breaks as well as chromosomal damage, in this paper, we aim to provide an overview of the impact of indoor air pollution on DNA damage and genome stability by reviewing the scientific papers that have used the comet, micronucleus, and γ-H2AX assays. These methods are valuable tools in human biomonitoring and for studying the mechanisms of action of various pollutants, and are readily used for the assessment of primary DNA damage and genome instability induced by air pollutants by measuring different aspects of DNA and chromosomal damage. Based on our search, in selected studies (in vitro, animal models, and human biomonitoring), we found generally higher levels of DNA strand breaks and chromosomal damage due to indoor air pollutants compared to matched control or unexposed groups. In summary, our systematic review reveals the importance of the comet, micronucleus, and γ-H2AX assays as sensitive tools for the evaluation of DNA and genome damaging potential of different indoor air pollutants. Additionally, research in this particular direction is warranted since little is still known about the level of indoor air pollution in households or public buildings and its impact on genetic material. Future studies should focus on research investigating the possible impact of indoor air pollutants in complex mixtures on the genome and relate pollutants to possible health outcomes. Keywords: indoor air quality, genome damage, comet assay, micronucleus assay, γ-H2AX assay, health risk Published in DiRROS: 06.11.2024; Views: 1060; Downloads: 552
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3. Evidence driven indoor air quality improvement : an innovative and interdisciplinary approach to improving indoor air qualityMario Lovrić, Goran Gajski, Jessica Fernández-Agüera, Mira Pöhlker, Bojana Žegura, Matjaž Novak, Alja Štern, Katja Kološa, Martina Štampar, 2024, review article Abstract: Indoor air pollution is a recognized emerging threat, claiming millions of lives annually. People are constantly exposed to ambient and indoor air pollution. The latest research shows that people in developed countries spend up to 90% of their time indoors and almost 70% at home. Although impaired IAQ represents a significant health risk, it affects people differently, and specific populations are more vulnerable: children, the elderly, and people with respiratory illnesses are more sensitive to these environmental risks. Despite rather extensive research on IAQ, most of the current understanding about the subject, which includes pollution sources, indoor–outdoor relationships, and ventilation/filtration, is still quite limited, mainly because air quality monitoring in the EU is primarily focused on ambient air quality and regulatory requirements are lacking for indoor environments. Therefore, the EDIAQI project aims to improve guidelines and awareness for advancing the IAQ in Europe and beyond by allowing user-friendly access to information about indoor air pollution exposures, sources, and related risk factors. The solution proposed with EDIAQI consists of conducting a characterization of sources and routes of exposure and dispersion of chemical, biological, and emerging indoor air pollution in multiple cities in the EU. The project will deploy cost-effective/user-friendly monitoring solutions to create new knowledge on sources, exposure routes, and indoor multipollutant body burdens. The EDIAQI project brings together 18 organizations from 11 different European countries that provide interdisciplinary skills and expertise in various fields, including environmental science and technology, medicine, and toxicology, as well as policy design and public engagement. Keywords: indoor air pollution, health risk, vulnerable populations, IAQ (Indoor Air Quality), EDIAQI project, monitoring solutions, exposure routes Published in DiRROS: 06.11.2024; Views: 1218; Downloads: 1906
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4. Report on economic justification of the project : LIFE Stop CyanoBloomMaja Zupančič Justin, Gorazd Lakovič, Tinkara Rozina, Bojan Sedmak, Marko Gerl, 2017, final research report Abstract: The Annexe 17 of the Final Report of the project LIFE Stop CyanoBloom, presenting the “Report on economic justification of the project” is composed of 14 chapters. The first part of the report gives a general overview of the cyanobacteria and their blooms, and species and toxicity of cyanobacterial toxins. With a literature review, economic consequences of cyanobacterial blooms on human health, commercial fishery, tourism and recreation, and lake monitoring and management have been evaluated. The chapter on phytoplankton and bacterioplankton monitoring options presents the importance of the phytoplankton for the lake ecology and evaluation of the water quality status. Traditional phytoplankton monitoring approaches are compared to the new monitoring options applied in the project. The chapter in-lake cyanobacterial control options give a broad review of available in-lake rehabilitation and remediation options, which can be compared with the electrochemical oxidation technique presented in the following chapter and applied in the project. According to the results obtained during the pilot testing activities, a cost evaluation of the approach has been performed and presented in the report. The last chapter gathers open issues and proposals for the further work in this field. Keywords: cyanobacteria, cyanotoxins, health risk Published in DiRROS: 05.09.2024; Views: 1146; Downloads: 740
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5. Innovative technology for cyanobacterial bloom control : LIFE Stop CyanoBloomMaja Zupančič Justin, Marko Gerl, Gorazd Lakovič, Bojan Sedmak, Tinkara Rozina, Neža Finžgar, Maja Čič, Mario Marinovič, Luka Teslić, Jošt Grum, Maša Čater, Tina Eleršek, Andrej Meglič, Andrey Yakuntsov, Lovro Pokorn, Tomaž Kralj, Matjaž Berčon, Branko Hamiti, 2017, final research report Abstract: Despite the efforts invested into measures to prevent water eutrophication, like prevention and treatment of point and dispersed discharges, the eutrophication still occurs. One of the unwanted consequences of eutrophic water state is the occurrence of excessive cyanobacterial blooms. Mass occurrence of cyanobacteria is a significant health risk related to surface waters in EU and worldwide since most of the cyanobacterial genera produce cyanotoxins. Besides toxicity, cyanobacterial blooms cause an increase in the turbidity of water and create taste and odour problems. All mentioned represents substantial economic losses in sectors like aquaculture, tourism, drinking water facilities and indirect losses due to increased healthcare expenditures and environmental degradation. It is, therefore, necessary to find appropriate solutions for rapid detection and also in-lake prevention of bloom occurrence despite, for example, existing high eutrophic conditions in the water body. The proposed project represents such a solution.
In the frame of the LIFE Stop CyanoBloom project, we have designed two solar powered robotic vessels for the in-lake detection and control of cyanobacterial proliferation. Each vessel is capable of three-dimensional localisation of cyanobacteria by measuring fluorescence of pigments involved in the process of photosynthesis. The vessels are additionally equipped with electrochemical cells using boron doped diamond electrodes fixed on board, producing short-lived hydroxyl radicals, which prevent cyanobacterial proliferation as well as cyanotoxins inactivation. Hydroxyl radicals cause different levels of damages to cyanobacterial cells as well as stress, that may also result in phage induced cyanobacterial lysis. Natural control of cyanobacterial density by lytic cyanophages is known for several years. The mixing of water during the electrolytic cell operation also increases the potential contacts of cyanobacteria with cyanophages, disturbs cyanobacterial buoyancy and generates additional nutrients for nontoxic phytoplankton population. Additional newly developed equipment of the vessel allows advanced automated navigation (automatic docking, avoiding obstacles, maintaining the position in windy conditions, etc.), sampling from different depths, real-time data transfer, statistical analysis and graphical presentation using the adequate software.
Keywords: cyanobacteria, cyanobacterial blooms, cyanotoxins, health risk Published in DiRROS: 05.09.2024; Views: 1187; Downloads: 1156
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6. A framework for the evaluation of biosecurity, commercial, regulatory, and scientific impacts of plant viruses and viroids identified by NGS technologiesSébastien Massart, Thierry Candresse, José Gil, Christophe Lacomme, Lukas Predajna, Maja Ravnikar, Jean-Sébastien Reynard, Artemis Rumbou, Pasquale Saldarelli, Dijana Škorić, Eeva J. Vainio, Jari P. T. Valkonen, Hervé Vanderschuren, Christina Varveri, Thierry Wetzel, 2017, original scientific article Abstract: Recent advances in high-throughput sequencing technologies and bioinformatics have generated huge new opportunities for discovering and diagnosing plant viruses and viroids. Plant virology has undoubtedly benefited from these new methodologies, but at the same time, faces now substantial bottlenecks, namely the biological characterization of the newly discovered viruses and the analysis of their impact at the biosecurity, commercial, regulatory, and scientific levels. This paper proposes a scaled and progressive scientific framework for efficient biological characterization and risk assessment when a previously known or a new plant virus is detected by next generation sequencing (NGS) technologies. Four case studies are also presented to illustrate the need for such a framework, and to discuss the scenarios. Keywords: NGS, pest risk analysis, virus diseases, biological characterization, plant health, regulatory agencies Published in DiRROS: 25.07.2024; Views: 1091; Downloads: 642
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7. Managing the deluge of newly discovered plant viruses and viroids : an optimized scientific and regulatory framework for their characterization and risk analysisNuria Fontdevila Pareta, Maryam Khalili, Ayoub Maachi, Mark Paul Selda Rivarez, Johan Rollin, Ferran Salavert Pamblanco, Coline Temple, Miguel A. Aranda, Denis Kutnjak, Maja Ravnikar, 2023, original scientific article Abstract: The advances in high-throughput sequencing (HTS) technologies and bioinformatic tools have provided new opportunities for virus and viroid discovery and diagnostics. Hence, new sequences of viral origin are being discovered and published at a previously unseen rate. Therefore, a collective effort was undertaken to write and propose a framework for prioritizing the biological characterization steps needed after discovering a new plant virus to evaluate its impact at different levels. Even though the proposed approach was widely used, a revision of these guidelines was prepared to consider virus discovery and characterization trends and integrate novel approaches and tools recently published or under development. This updated framework is more adapted to the current rate of virus discovery and provides an improved prioritization for filling knowledge and data gaps. It consists of four distinct steps adapted to include a multi-stakeholder feedback loop. Key improvements include better prioritization and organization of the various steps, earlier data sharing among researchers and involved stakeholders, public database screening, and exploitation of genomic information to predict biological properties. Keywords: plant viruses and viroids, high throughput sequencing (HTS), biological characterization, plant health, regulatory agencies, Pest Risk Analysis (PRA), virus disease Published in DiRROS: 12.07.2024; Views: 1497; Downloads: 699
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8. Content of trace elements and human health risk assessment via consumption of commercially important fishes from Montenegrin coastNeda Bošković, Danijela Joksimović, Oliver Bajt, 2023, original scientific article Abstract: Muscle tissues of Mullus barbatus and Merluccius merluccius were analyzed for the presence of selected trace elements (As, Hg, Cd, and Pb) to determine the value of the daily intake of trace elements from fish consumption and to assess the risk to human health. The mean concentrations of As in the muscle tissue of M. barbatus and M. merluccius for the entire period were 19.689 mg/kg wet weight (ww) and 8.356 mg/kg ww, Hg 0.497 mg/kg ww and 0.153 mg/kg ww, and Pb 0.031 mg/kg ww and 0.025 mg/kg ww, respectively. The concentrations of Cd in all fish sampled were below the detection limit (<0.02 mg/kg ww). The evaluation of the potential health risk assessments based on the target hazard quotient (THQ) and estimated daily intake (EDI) indicated that the intake of As in both fish species and Hg for M. barbatus could pose an appreciable risk to human health. The calculated hazard index (HI) was higher than 1 for both fish species. The continuous monitoring of trace elements’ concentrations in fish is strongly recommended, as the results demonstrate potential health risks due to the presence of As and Hg. Keywords: trace elements, Mullus barbatus, Merluccius merluccius, human health risk assessment, Montenegro, Adriatic Sea, hydrobiology Published in DiRROS: 12.07.2024; Views: 1245; Downloads: 735
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9. Documenting metrological traceability as intended by ISO 15189:2012 : a consensus statement about the practice of the implementation and auditing of this norm elementMarc H. M. Thelen, Florent Vanstapel, Pika Meško-Brguljan, Bernard Gouget, Guilaine Boursier, Edward Barrett, Christos Kroupis, Maria Lohmander, Luděk Šprongl, Tatjana Vodnik, 2019, original scientific article Abstract: ISO15189:2012 requires medical laboratories to document metrological traceability of their results. While the ISO17511:2003 standard on metrological traceability in laboratory medicine requires the use of the highest available level in the traceability chain, it recognizes that for many measurands there is no reference above the manufacturer's selected measurement procedure and the manufacturer's working calibrator. Some immunoassays, although they intend to measure the same quantity and may even refer to the same reference material, unfortunately produce different results because of differences in analytical selectivity as manufacturers select different epitopes and antibodies for the same analyte. In other cases, the cause is the use of reference materials, which are not commutable. The uncertainty associated with the result is another important aspect in metrological traceability implementation. As the measurement uncertainty on the clinical samples is influenced by the uncertainty of all steps higher in the traceability chain, laboratories should be provided with adequate and appropriate information on the uncertainty of the value assignment to the commercial calibrators that they use. Although the between-lot variation in value assignment will manifest itself as part of the long-term imprecision as estimated by the end-user, information on worst-case to be expected lot-lot variation has to be communicated to the end-user by the IVD provider. When laboratories use ancillary equipment that potentially could have a critical contribution to the reported results, such equipment needs verification of its proper calibration and criticality to the result uncertainty could be assessed by an approach based on risk analysis, which is a key element of ISO15189:2012 anyway. This paper discusses how the requirement for metrological traceability as stated in ISO15189 should be met by the medical laboratory and how this should be assessed by accreditation bodies. Keywords: laboratories, medical laboratory science, risk management, quality control, quality improvement, health care quality assurance, metrological traceability Published in DiRROS: 22.10.2020; Views: 3136; Downloads: 662
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