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1.
Phytosociological analysis of Gladiolus palustris sites in northwestern, western and southwestern Slovenia
Igor Dakskobler, Andrej Seliškar, Branko Vreš, 2021, original scientific article

Abstract: With a phytosociological analysis of more than 200 relevés on more than 25 localities with Gladiolus palustrisand (or) G. illyricusin western and southwestern Slovenia we identified more than 15 communities of association rank that belong to at least eight vegetation alliances and five classes. We determined that these two species can grow on the same localities and sites, but Gladiolus palustrisflowers at least 14 days after G. illyricus. Another reliable distinguishing characteristic is the fibrous tunic covering the corm. Gladiolus palustris is threatened in most of the examined localities, except for the Banjšice Plateau and the Slavnik range in Čičarija, but the current nature conservation policy in Slovenia does not provide for its preservation.
Keywords: Gladiolus palustris, G. illyricus, phytosociology, syntaxonomy, Natura 2000, nature conservation, Slovenia
Published in DiRROS: 14.01.2026; Views: 51; Downloads: 36
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2.
Multifactorial analysis of wood deterioration in ancient Egypt : a case study of Khufu’s Second Solar Boat
Shimaa Ibrahim, Paola Grenni, Lucia Mancini, Marco Voltolini, Hanan Mohamed Kamal Abdel-Fatah, Ahmed Refaat, Dina M. Atwa, 2025, original scientific article

Abstract: This study presents a multi-analytical investigation of the wooden components from Khufu’s Second Solar Boat, one of the valuable archaeological discoveries in ancient Egypt. The research integrates advanced imaging and analytical techniques to identify wood species, assess deterioration patterns, and characterize the fungal and bacterial biodeteriogens. The initial visual examination documented the state of preservation at the time of discovery. Subsequently, the identification of the wood species was carried out. The deterioration status was assessed using a variety of tools, including scanning electron microscopy (SEM) and high-resolution synchrotron radiation computed microtomography (SR-μCT) for morphological alterations, X-ray diffraction (XRD) for crystallinity changes and Fourier-transform infrared spectroscopy (FTIR) to assess chemical degradation indexes of wood. Moreover, molecular techniques were used to identify and characterize the presence of biodeterioration agents. Results indicate that ancient craftsmen used cedar wood for the boat construction. The analysed samples exhibited advanced biotic and abiotic degradation, as evidenced by microbiological assessments, XRD measurements of cellulose crystallinity, FTIR indices, SEM micrographs and SR-μCT data. These results provide crucial insights into the long-term degradation processes of archaeological wood in arid environments, enhancing our understanding of ancient Egyptian woodworking practices and informing future conservation strategies for similar artifacts.
Keywords: archaeological wood conservation, multi-analytical characterization, biodeterioration analysis, ancient egyptian woodworking, x-ray computed microtomography, lebanon cedar
Published in DiRROS: 13.01.2026; Views: 59; Downloads: 29
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3.
Growth rates of the critically endangered fan mussel Pinna nobilis in the Slovenian sea (northern Adriatic)
Valentina Pitacco, Ana Fortič, Domen Trkov, Borut Mavrič, Ciril Mlinar, Lovrenc Lipej, 2025, original scientific article

Abstract: The fan mussel Pinna nobilis is a bivalve endemic to the Mediterranean Sea that is listed as Critically Endangered on the IUCN Red List. Its decline is due to a disease that has led to mass mortalities throughout the Mediterranean. The Gulf of Trieste (northern Adriatic) used to harbour one of the densest populations of P. nobilis, but since 2019, it has also been affected by this disease. The aim of this work was to analyse the growth of P. nobilis in the Slovenian part of the Gulf, with 3 case studies: (1) in situ measurement of adults before the mass mortality event (MME), (2) in situ measurement of juveniles after the MME and (3) ex situ measurement of juveniles kept in the laboratory. The pre-MME growth rate was comparable to that in other areas of the Gulf. In the first years of life, the growth rate of P. nobilis is higher than the growth rate reported in the southern Adriatic, and comparable to the growth rate in the Spanish lagoons. This is probably related to the physico-chemical conditions and confirms the potential of the area for restoration actions. Captive growth of juveniles could be an effective conservation measure, although juveniles kept in the laboratory grow more slowly than those in the sea, probably because of their lower immune response. Temperature plays an ambivalent role, as it increases the growth rate but also triggers the parasites responsible for the MME, which is of concern, given the recent rise of seawater temperatures and heatwaves.
Keywords: fan mussel, model growth, temperature, conservation, northern Adriatic sea
Published in DiRROS: 23.12.2025; Views: 200; Downloads: 94
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4.
Conservation hints for Pinna nobilis from a century-old genetic time capsule
Ilenia Azzena, Chiara Locci, Noemi Pascale, Ilaria Deplano, Riccardo Senigaglia, Edoardo Batistini, Daniela Caracciolo, Mariachiara Chiantore, Saul Ciriaco, Maria Paola Ferranti, Daniele Grech, Arianna Liconti, Monica Montefalcone, Alice Oprandi, Valentina Pitacco, Marco Segarich, Rym Zakhama-Sraieb, Ahmed Ben Hmida, Salma Zribi, Fabio Scarpa, Marco Casu, Daria Sanna, 2025, original scientific article

Abstract: The noble pen shell, Pinna nobilis, is an iconic marine bivalve endemic to the Mediterranean Sea, playing a key role as an ecosystem engineer. Over the past century, it has faced severe threats from overharvesting, pollution, and catastrophic mass mortality events. This study analysed 119 mitochondrial COI gene sequences from historical (1700s, 1920s, 1970s, 1990s) and modern (2000s) samples, including survivors of recent mass mortality crises. We standardised a protocol to extract DNA from ancient byssus samples over a century old and dated the emergence of the mitochondrial lineages of Pinna nobilis, uncovering its evolutionary history in unprecedented detail. Our findings suggest two main temporal origins for the species’ genetic variation: (i) a group of modern lineages directly descended from Pinna nobilis early ancestors originating 2.5 mya, and (ii) a large group derived from the first Pleistocene radiation of the species, approximately 1.5 mya. Importantly, our research depicts the evolutionary response of Pinna nobilis to three major challenges in the last century: human overexploitation, pollution, and environmental changes. Our results highlight the species’ remarkable resilience, likely mediated by Pleistocene genetic traits, whose persistence over time mainly depends on the maintaining of a high effective population size to ensure successful recruitment.
Keywords: noble pen shell, genetic variability, evolutionary response, species conservation
Published in DiRROS: 19.11.2025; Views: 256; Downloads: 145
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5.
A contribution to the vascular and bryophyte flora of the upper Neretva and Zalomka river valleys (Bosnia and Herzegovina)
Đorđije Milanović, Simona Strgulc Krajšek, Jugoslav Brujić, Valentin Heimer, Dragan Koljanin, Marija Kravanja, Ivana Rešetnik, Vladimir Stupar, Peter Schönswetter, Božo Frajman, 2025, original scientific article

Abstract: etween 1st and 6th June 2023, we conducted floristic research on sev-eral sites in the upper Neretva River valley and along Zalomka River at Nevesinjsko Polje. We recorded 933 taxa in total; 897 plant taxa including 125 bryophytes were found at 51 localities in the upper Neretva River val-ley, and 200 plant taxa, including 32 bryophytes, were found at 11 sites in the Zalomka River valley. Among the registered taxa, 39 taxa are endemic to the Balkan Peninsula, 56 are listed in the List of endangered and rare taxa of Bosnia and Herzegovina, 27 are on the Red List of the Federation of Bosnia and Herzegovina, and 95 taxa are protected in the Republic of Srpska. We also registered several species new to the flora of Bosnia and Herzegovina. Among Angiosperms these were Barbarea intermedia, Lamium hybridum, Myosotis sicula and Poa ursina, and among bryophytes Fissidens gracilifolius, Grimmia dissimulata, Plagiomnium ellipticum, Schistidium robustum, Sphenolobus minutus, Tortella fasciculata and T. pseudofragilis. Our study reveals the high nature conservation value of the upper Neretva and Zalomka River valleys that should be protected for future generations. In addition, further systematic research of the flora of Bosnia and Herzegovina is needed
Keywords: angiosperms, mosses, nature conservation, Neretva River, vegetation, are species
Published in DiRROS: 16.10.2025; Views: 292; Downloads: 125
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6.
Sensory pollutants have negative but different effects on nestbox occupancy and breeding performance of a nocturnal raptor across Europe
Giuseppe Orlando, Luca Nelli, Paul Baker, Patrik Karell, Al Vrezec, 2026, original scientific article

Abstract: Anthropogenic noise and artificial light at night (ALAN) are expanding globally, acting as pervasive sensory pollutants that can disrupt wildlife behaviour and reproduction. While most research has focused on diurnal species, the effects of these pollutants on the ecological response of nocturnal predators remain poorly understood. Using data from nine European countries, we investigated the effects of traffic noise, ALAN, and road proximity on nestbox occupancy and reproduction in the Tawny Owl (Strix aluco), a nocturnal raptor widespread across Europe. Traffic noise consistently reduced both nestbox occupancy and reproductive success regardless of road proximity. ALAN also impaired occupancy and reproduction, but its negative effect on reproduction changed based on the proximity to roads. Interestingly, the negative effect of ALAN was stronger in sites further from roads, but it attenuated in their proximity, where owls' hatching success and brood size moderately improved. This finding suggests that near roads, where prey abundance and availability are also generally high, owls may either find the prey regardless of ALAN or they may exploit it to facilitate hunting and brood provisioning. However, vicinity to roads might enhance mortality by vehicle collisions, which represents one of the greatest threats for the conservation of owls. Our findings highlight that anthropogenic noise and the co-occurrence between ALAN and roads can affect settlement decisions and breeding performance in nocturnal raptors, with potential consequences across the food chain. Mitigating anthropogenic noise and promoting nighttime-lighting systems that minimize owls' presence close to roads will represent valuable actions to improve their conservation.
Keywords: ALAN, anthropogenic noise, owls, reproductive fitness, roadside habitats, raptor conservation, conservation biology
Published in DiRROS: 14.10.2025; Views: 255; Downloads: 118
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7.
Recruitment and controlled growth of juveniles of the critically endangered fan mussel Pinna nobilis in the Northern Adriatic
Valentina Pitacco, Domen Trkov, Daniela Caracciolo, Saul Ciriaco, Marco Segarich, Edoardo Batistini, Martina Orlando-Bonaca, Lovrenc Lipej, Borut Mavrič, Manja Rogelja, Ilenia Azzena, Chiara Locci, Fabio Scarpa, Daria Sanna, Marco Casu, Chiara Manfrin, Alberto Pallavicini, Ana Fortič, 2025, original scientific article

Abstract: The fan mussel Pinna nobilis is the largest bivalve species in the Mediterranean Sea and provides numerous ecosystem services. It is classified as critically endangered by IUCN (International Union for Conservation of Nature) due to severe mass mortality events throughout the Mediterranean. The aims of this work are as follows: (i) to assess the current recruitment potential of the species, (ii) to enhance recruitment by keeping juveniles in controlled conditions before releasing them back into the sea, and (iii) to assess the health status of recruits. In the period 2022–2023, larval collectors were set up in the Gulf of Trieste as part of the LIFE Pinna project. The collected individuals were kept in aquaria in two different facilities under different conditions: (a) a closed system with constant water temperature, live phytoplankton, and commercial food and (b) an open system with ambient seawater temperature and commercial food. A clear temporal and spatial variability in recruitment was observed: 13 recruits were found in 2022 and 50 recruits in 2023. The live specimens were between 0.5 and 8 cm in size upon collection and larger in 2023. The growth and survival rate did not differ significantly between the two systems, but the average monthly growth and survival rate were related to the initial size of the juveniles.
Keywords: pen shell, ex situ maintenance, in situ maintenance, Haplosporidium pinnae, Mycobacterium, conservation, marine biology
Published in DiRROS: 08.10.2025; Views: 311; Downloads: 134
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8.
9.
Structural and compositional indicators of the conservation status of forest habitats : a case study of ravine forests – EU priority habitat type Tilio-Acerion
Lado Kutnar, Janez Kermavnar, Anže Martin Pintar, 2025, original scientific article

Abstract: Maintaining the conservation status of habitat types such as the ravine forests (Tilio-Acerion) assessed in this study is a priority of the European Natura 2000 network. Ravine forests often occur in smaller, fragmented areas, but are widely distributed throughout European forests. Reliable indicators of the conservation status of Natura 2000 habitats, which support monitoring and reporting under Article 17 of the Habitats Directive, are often not available. Therefore, we tested a set of 161 structural and compositional variables as potential indicators of the conservation status of close-to-nature managed ravine forests in a Natura 2000 site in eastern Slovenia. The studied forests ranged from Acer pseudoplatanus-dominated stands to those dominated by Fraxinus excelsior or Tilia species. Most forests were classified as having either a favourable or inadequate conservation status. The main pressures included game browsing and mortality of the key tree species, primarily caused by invasive alien fungi. Favourable conservation status was associated with less intensively managed Tilia-dominated stands on rocky ridges and steep slopes. It was also linked to higher tree layer cover, particularly of Acer pseudoplatanus, in well-preserved forest stands. Conversely, indicators of bad conservation status were associated with Fraxinus excelsior-dominated stands that had been severely affected by invasive alien fungi, resulting in increased volumes of standing and lying deadwood. The resulting tree mortality created more open stand canopies with increased light availability at the forest floor, as indicated by the higher number of plant species in the herb and shrub layer. The conservation status of ravine forests is likely to be increasingly threatened by the adverse effects of climate change, including pests and disease outbreaks and other disturbances. To ensure the continued favourable conservation status of ravine forests, it is essential to monitor key indicators and apply appropriate forest management measures.
Keywords: forest habitats, vegetation, pressures, conservation, indicators, Eastern Slovenia, forest stands
Published in DiRROS: 09.09.2025; Views: 403; Downloads: 167
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10.
The genetic consequences of population marginality : a case study in maritime pine
Adélaïde Theraroz, Santiago C. González-Martínez, Carlos Guadaño-Peyrot, Juliette Archambeau, Sara Pinosio, Francesca Bagnoli, Andrea Piotti, Camilla Avanzi, Giovanni G. Vendramin, Ricardo Alía, Delphine Grivet, Marjana Westergren, 2024, complete scientific database of research data

Abstract: This dataset was produced by a consortium of European research Intitutes. For the data collection, needles were sampled from a total of 1,510 individuals from 82 maritime pine populations covering all previously identified gene pools throughout the species range. 10,185 SNPs were genotyped from the multispecies 4TREE SNPs chip (chip produced within the framework of the B4est project). This dataset was used to carry out a population genetics study.
Keywords: population genetics, conservation genetics, marginal populations, Pinus pinaster, genetic indicators
Published in DiRROS: 08.07.2025; Views: 402; Downloads: 313
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