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Query: "keywords" (carbohydrates) .

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1.
Phloem : a missing link in understanding tree growth response in a changing environment
Jožica Gričar, 2024, original scientific article

Abstract: Although the activities of various tree organs and tissues are under different environmental and internal constraints, they need to be coordinated to ensure whole-tree functioning. Dusart et al. (2024) conducted a heating experiment on branches of Juglans regia saplings during the entire growing season to examine the effect of temperature on primary (bud) and secondary (xylem) growth. They found that primary and secondary meristems responded asynchronously to elevated temperature, which suggests that lack of coordination mechanisms between primary and secondary growth at crown scale could have a potential impact on canopy and tree architecture.
Keywords: phloem, wood anatomy, tree growth, angiosperms, bark, meristem, non-structural carbohydrates, primary growth, radial growth, secondary growth, temperature, xylem
Published in DiRROS: 05.12.2024; Views: 1059; Downloads: 887
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2.
Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissues
Elizabeth Dunn Covington, Thomas Roitsch, Marina Dermastia, 2016, original scientific article

Abstract: Physiological studies in plants often require enzyme extraction from tissues containing high concentrations of phenolsand polyphenols. Unless removed or neutralized, such compounds may hinder extraction, inactivate enzymes, and inter-fere with enzyme detection. The following protocol for activity assays for enzymes of primary carbohydrate metabo-lism, while based on our recently published one for quantitative measurement of activities using coupled spectrophoto-metric assays in a 96-well format, is tailored to the complexities of phenolic- and anthocyanin-rich extracts from grape-vine leaf. As a case study we applied the protocol to grapevine leaf samples infected with plant pathogenic bacteriašCandidatusPhytoplasma solani’, known to alter carbohydrate metabolism in grapevine. The described adaptations maybe useful for determination of metabolic fingerprints for physiological phenotyping of other plant species with inhe-rently high levels of phenolic compounds.
Keywords: AGPase, carbohydrates, invertases, sucrose synthase, panel of enzyme activity assays, phytoplasma
Published in DiRROS: 25.07.2024; Views: 1335; Downloads: 744
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