1. Employing microalga Chlorella sorokiniana in the biosynthesis of paramagnetic and catalytically functional manganese clusterMarija Tanović, Milan Žižić, Milica Milenković, Zvonko Jagličić, Wilfred Hagen, Marina Stanić, Dalibor M. Stanković, Snežana Kovačević, Dmitry Karpov, Primož Šket, Uroš Javornik, Ivan Spasojević, Milena Dimitrijević, 2025, original scientific article Keywords: biosynthesis, manganese, microalgae
Published in DiRROS: 13.06.2025; Views: 182; Downloads: 91
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2. Anthocyanin and proanthocyanidin evolution during ripening of the Refošk grapevine is modulated by deficit irrigationPaolo Sivilotti, Alberto Calderan, Riccardo Braidotti, Antonio Filippi, Marco Vuerich, Enrico Peterlunger, Alessia Cogato, Dejan Bavčar, Klemen Lisjak, Andreja Vanzo, 2024, original scientific article Keywords: vine water stress, polyphenol biosynthesis, proanthocyanidin polymerization, galloylation, anthocynin
Published in DiRROS: 20.12.2024; Views: 354; Downloads: 101
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3. Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosusAlen Pšeničnik, Lucija Slemc, Martina Avbelj, Miha Tome, Martin Šala, Paul R. Herron, Maksym Shmatkov, Marko Petek, Špela Baebler, Peter Mrak, Daslav Hranueli, Antonio Starcevic, Iain S. Hunter, Hrvoje Petković, 2024, original scientific article Abstract: Most biosynthetic gene clusters (BGC) encoding the synthesis of important microbial secondary metabolites, such as antibiotics, are either silent or poorly expressed; therefore, to ensure a strong pipeline of novel antibiotics, there is a need to develop rapid and efficient strain development approaches. This study uses comparative genome analysis to instruct rational strain improvement, using Streptomyces rimosus, the producer of the important antibiotic oxytetracycline (OTC) as a model system. Sequencing of the genomes of two industrial strains M4018 and R6-500, developed independently from a common ancestor, identified large DNA rearrangements located at the chromosome end. We evaluated the effect of these genome deletions on the parental S. rimosus Type Strain (ATCC 10970) genome where introduction of a 145 kb deletion close to the OTC BGC in the Type Strain resulted in massive OTC overproduction, achieving titers that were equivalent to M4018 and R6-500. Transcriptome data supported the hypothesis that the reason for such an increase in OTC biosynthesis was due to enhanced transcription of the OTC BGC and not due to enhanced substrate supply. We also observed changes in the expression of other cryptic BGCs; some metabolites, undetectable in ATCC 10970, were now produced at high titers. This study demonstrated for the first time that the main force behind BGC overexpression is genome rearrangement. This new approach demonstrates great potential to activate cryptic gene clusters of yet unexplored natural products of medical and industrial value.
Keywords: genome reduction, antibiotic biosynthesis, oxytetracycline, cryptic metabolites
Published in DiRROS: 07.08.2024; Views: 688; Downloads: 515
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4. Triangular in vivo self-assembling coiled-coil protein origamiSabina Božič Abram, Helena Gradišar, Jana Aupič, Adam Round, Roman Jerala, 2021, original scientific article Keywords: coiled coil, enzyme clustering, biosynthesis, resveratrol, mevalonate
Published in DiRROS: 22.03.2021; Views: 2665; Downloads: 1321
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5. Cryo-EM structure of the archaeal 50S ribosomal subunit in complex with initiation factor 6 and implications for ribosome evolutionBasil J. Greber, Daniel Boehringer, Vlatka Godinić Mikulčić, Ana Crnković, Michael Ibba, Ivana Weygand-Durašević, Nenad Ban, 2012, original scientific article Keywords: archaeal proteins, cryoelectron microscopy, Methanobacteriaceae, prokaryotic initiation, factors, protein biosynthesis, ribosome subunits, archaeal, ribosomes
Published in DiRROS: 07.05.2020; Views: 2486; Downloads: 1571
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6. RNA-dependent cysteine biosynthesis in bacteria and archaeaTakahito Mukai, Ana Crnković, Takuya Umehara, Natalia N. Ivanova, Nikos C. Kyrpides, Dieter Söll, 2017, original scientific article Keywords: amino acyl-tRNA synthetases, archaea, archaeal proteins, bacteria, computational biology, crystallography, X-ray, genetic code, genome, archaeal, genome, bacterial, phosphoserine, protein binding, protein biosynthesis, RNA, archaeal, RNA, bacterial, RNA, transfer, amino acyl, RNA, transfer, cys, sulfur/metabolism, biochemistry, bioinformatics, cysteine biosynthesis, genetic code, translation
Published in DiRROS: 07.05.2020; Views: 2590; Downloads: 1729
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