1. Folikulitis masažnih bazenovIza Vodlan, Mateja Starbek Zorko, 2026, other scientific articles Abstract: Folikulitis masažnih bazenov (angl. hot tub folliculitis) ali Pseudomonas folikulitis je bakterijska okužba dlačnih mešičkov, ki se navadno pojavi po uporabi masažnih bazenov (jacuzzi). Pogosto se lahko pojavi v izbruhih, ki prizadenejo več posameznikov, ki so uporabljali isti onesnažen vodni vir. Prikazujemo primer 11-letnega dečka, ki je bil napoten na Dermatovenerološko kliniko zaradi nenadnega pojava nesrbečih papul po koži telesa. Kasneje smo ob anamnezi izvedeli, da so se enake kožne spremembe pojavile pri vseh družinskih članih po uporabi masažnega bazena.
Keywords: folikulitis masažnih bazenov, Pseudomonas aeruginosa, masažni bazen, papule, pustule
Published in DiRROS: 06.05.2026; Views: 193; Downloads: 114
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2. Genomic virulence markers are associated with severe outcomes in patients with Pseudomonas aeruginosa bloodstream infectionJohn Karlsson Valik, Christian G Giske, Badrul Hasan, Mónica Gozalo-Margüello, Luis Martínez-Martínez, Manica Müller-Premru, Žiga Martinčič, Bojana Beović, Sofia Maraki, 2024, original scientific article Abstract: Background: Pseudomonas aeruginosa (PA) bloodstream infection (BSI) is a common healthcare-associated complication linked to antimicrobial resistance and high mortality. Ongoing clinical trials are exploring novel anti-virulence agents, yet studies on how bacterial virulence affects PA infection outcomes is conflicting and data from real-world clinical populations is limited. Methods: We studied a multicentre cohort of 773 adult patients with PA BSI consecutively collected during 7-years from sites in Europe and Australia. Comprehensive clinical data and whole-genome sequencing of all bacterial strains were obtained. Results: Based on the virulence genotype, we identify several virulence clusters, each showing varying proportions of multidrug-resistant phenotypes. Genes tied to biofilm synthesis and epidemic clones ST175 and ST235 are associated with mortality, while the type III secretion system is associated with septic shock. Adding genomic biomarkers to machine learning models based on clinical data indicates improved prediction of severe outcomes in PA BSI patients. Conclusions: These findings suggest that virulence markers provide prognostic information with potential applications in guiding adjuvant sepsis treatments.
Keywords: Pseudomonas aeruginosa, bloodstream infection, virulence
Published in DiRROS: 27.02.2026; Views: 515; Downloads: 283
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3. Succinylation of polyallylamine: influence on biological efficacy and the formation of electrospun fibersLucija Jurko, Matej Bračič, Silvo Hribernik, Damjan Makuc, Janez Plavec, Filip Jerenec, Sonja Žabkar, Nenad Gubeljak, Alja Štern, Rupert Kargl, 2021, original scientific article Abstract: Succinylation of proteins is a commonly encountered reaction in biology and introduces negatively charged carboxylates on previously basic primary amine groups of amino acid residues. In analogy, this work investigates the succinylation of primary amines of the synthetic polyelectrolyte polyallylamine (PAA). It investigates the influence of the degree of succinylation on the cytotoxicity and antibacterial activity of the resulting polymers. Succinylation was performed in water with varying amounts of succinic anhydride and at different pH values. The PAA derivatives were analyzed in detail with respect to molecular structure using nuclear magnetic resonance and infrared absorbance spectroscopy. Polyelectrolyte and potentiometric charge titrations were used to elucidate charge ratios between primary amines and carboxylates in the polymers. The obtained materials were then evaluated with respect to their minimum inhibitory concentration against Staphylococcus aureus and Pseudomonas aeruginosa. The biocompatibility was assessed using mouse L929 fibroblasts. The degree of succinylation decreased cytotoxicity but more significantly reduced antibacterial efficacy, demonstrating the sensitivity of the fibroblast cells against this type of ampholytic polyelectrolytes. The obtained polymers were finally electrospun into microfiber webs in combination with neutral water-soluble polyvinyl alcohol. The resulting non-woven could have the potential to be used as wound dressing materials or coatings.
Keywords: polyallylamine hydrochloride, succinylation, aqueous chemistry, cytotoxicity, antimicrobial effect, electrospinning, nanofibers, mouse L929 fibroblasts, Staphylococcus aureus, Pseudomonas aeruginosa
Published in DiRROS: 19.07.2024; Views: 1273; Downloads: 1164
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