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1.
Multivariate statistical methods in determining the spatial distribution of chemical elements in soil from the Mavrovo-Rostuše region, North Macedonia
Trajče Stafilov, Robert Šajn, Kristina Petrovska, 2023, original scientific article

Abstract: In this work, the contents and spatial distributions of 19 elements (Ag, Al, B, Ba, Ca, Cr, Cu, Fe, K, Li, Mg, Mn, Na, Ni, P, Pb, Sr, V, Zn) in the soil of the western part of North Macedonia (Mavrovo-Rostuše region) are presented. For this purpose, a total of 66 soil samples were collected from 33 locations (33 samples of topsoil, 0-5 cm, and 33 samples of subsoil, 20-30 cm). All samples were analysed by inductively coupled plasma - atomic emission spectrometry (ICP-AES) after complete digestion with four acids (HNO3, HF, HClO4 and HCl). The obtained results were statistically evaluated and spatial distribution maps for all analysed elements were also prepared. Factor analysis was performed to reduce the number of data used and new synthetic variables (factors) were identified. Through the application of factor analysis, three geochemical associations were identified: Factor 1 (Zn, K, Cu, Fe, and Li), Factor 2 (Cr, Ni, and Mg) and Factor 3 (Ca and Al). From the obtained data and the maps of spatial distribution, it could be concluded that the distribution of the analysed elements is related to the lithology of the region. Thus, it was found that the higher content of elements of Factor 1 occurs in the eastern and southern part of the study area (middle and lower reaches of the Radika River and along the Mala River), where Mesozoic and Paleozoic carbonates as well as Paleozoic shales and Paleogene flysch prevail. Factor 2 (Cr, Ni and Mg) also represents a lithogenic association. The highest contents of the elements in both soil layers were found in the areas where Paleozoic sandstones and shales (village of Lazaropole and the area around the Mavrovo Lake) and Paleogene flysch (Rostuše village) predominate. Factor 3 (Ca and Al) also represents lithogenic association of elements. The highest content of these elements was found in the northwestern part of the study area (village of Žirovnica and along the Berička River) and in the northeastern region above the village of Brodec where Mesozoic carbonates and Paleogene flysch dominate.
Keywords: soil, heavy metals, spatial distribution, Mavrovo-Rostuše region, North Macedonia
Published in DiRROS: 04.01.2024; Views: 173; Downloads: 41
.pdf Full text (1,90 MB)

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Teaching and learning the language of the neighbour country : tools for mainstream primary education in the Slovenian-Italian border area
Irina Moira Cavaion, 2022, original scientific article

Abstract: The article presents two central tools for teaching neighbour languages in border areas within the didactic framework of the Contact-Based-(Neighbour-)Language-Learning-andTeaching method (CoBLaLT, Cavaion 2015; 2016; 2019), namely a teacher’s guide to contact-based neighbour language teaching and the digital portfolio ‘My multimedia autobiography of crossborder contacts’ for pupils aged 11 to 15. They were developed as part of a postdoctoral project between 2017-2019 in the Italian-Slovenian border region. The article highlights the importance of making language teaching research a collaborative process between a strong scientific and professional community that thus fosters innovation and effectiveness of methods and contents in neighbour language learning.
Keywords: neighbourg langages, teaching, learning, multilingual border region, linguistic autobiography, European language policy
Published in DiRROS: 12.04.2022; Views: 502; Downloads: 266
.pdf Full text (1,32 MB)
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4.
PCR primers comparisons for a successful Tuber spp. DNA region amplification in routine identifications
Tina Unuk Nahberger, Hojka Kraigher, Tine Grebenc, 2020, original scientific article

Abstract: Since late 20th century DNA sequencing became the method of choice method in precision species identification. The ITS region is one of the official fungal barcoding DNA markers, although in some cases sequencing of the ITS re-gion may, due to misidentification, mislabeling or nomen-clature errors in public databases, lead to incorrect or insuf-ficient identification, as is currently a case in the genus Tu b e r. The aim of this study was to test, which ITS primer pairs are most appropriate and optimal for Tu b e r species DNA region amplification. Thereby we (1) compared ampli-fication success for different Tu b e r species using fungal spe-cific primer pair ITS1f and ITS4 and (2) compared amplifi-cation success using different ITS primer pair combinations in amplifying DNA region an example species Tuber aesti-vum. Based on results, Tuber aestivum was one of the most reluctant Tu b e r species in this study and in most cases failed to amplify with the above primer pair. After comparing dif-ferent ITS primer pairs, we conclude that the primer pair ITS5 and ITS7 is the most appropriate primer pair for ampli-fication DNA region of T. ae stiv um as it resulted in high am-plification success from ectomycorrhizal root tips. Based on sequences, gained from public databases, we found that ITS1f and ITS6 primers have a mismatch in one base pair compared to the target sequence of Tuber aestivum, thus re-sulting in poor or no amplification success. Although prim-er pair ITS5 and ITS7 in our study was proven to be the most appropriate primer pair in amplifying DNA region Tu b e r aestivum species, further analysis about appropriateness of it for a general barcoding and identification of ectomycorrhiza in complex community samples is needed.
Keywords: Tuber spp., ITS region, PCR amplification, ITS primers
Published in DiRROS: 30.07.2020; Views: 1580; Downloads: 1165
.pdf Full text (6,81 MB)
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Analysis of the influece of ungulates on the regeneration of Dinaric fir-beech forests in the research site Trnovec in the Kočevje forest managementregion
Kristjan Jarni, Dušan Robič, Andrej Bončina, 2004, original scientific article

Abstract: In the period 1970-2000, 152 fenced-in areas were built in the Kočevje Forest Management Region with an aim to protect tree seedlings and saplings from ungulatesć activity and to monitor the influence of roe and red deer on natural regeneration. The average surface area of fenced areas is 0.71 ha. Using the pair comparison technique (fenced vs. unfenced areas), the structureand the composition of the natural regeneration of tree species as well as complete shrub and herb vegetation were analysed in the research site Trnovec. Furthermore, the vegetation was investigated using the Braun-Blanquetmethod. The research results show significant differences between fenced and unfenced areas, both in tree species composition and in theheight structure of the sapling community. In fenced areas the total numberof saplings taller than 50 cm is higher and an increase is also evident in the number of saplings of silver fir Abies alba, sycamore Acer pseudoplatanus, elm Ulmus glabra and other minor tree species. There are also significant differences in species composition and in the abundance of plant species in the herb layer. The results show that natural regeneration of Dinaric fir-beech forests is successful, provided the influence of ungulates is excluded.
Keywords: natural forest regeneration, Fagus sylvatica, roe deer, fir-beech forest, Abies alba, fenced area, seedling browsing, Kočevje forest region, research site Trnovec
Published in DiRROS: 12.07.2017; Views: 4455; Downloads: 2006
.pdf Full text (420,75 KB)

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