Phytochemicals and their correlation with molecular data in micromeria and Clinopodium (Lamiaceae) taxaDario Kremer
, Valerija Dunkić
, Ivan Radosavljević
, Faruk Bogunić
, Daniella Ivanova
, Dalibor Ballian
, Danijela Stešević
, Vlado Matevski
, Vladimir Ranđelović
, Eleni Eleftheriadou
, Zlatko Šatović
, Zlatko Liber
, 2022, original scientific article
Abstract: A study of the phytochemical and molecular characteristics of ten Micromeria and six Clinopodium taxa (family Lamiaceae) distributed in the Balkan Peninsula was carried out. The phytochemicals detected in essential oils by gas chromatography, mass spectrometry, and molecular data amplified fragment length polymorphism were used to study the taxonomic relationships among the taxa and the correlations between phytochemical and molecular data. STRUCTURE analysis revealed three genetic groups, while Bayesian Analysis of Population Structure grouped the studied taxa into 11 clusters nested in the groups obtained by STRUCTURE. Principal components analysis performed with the 21 most represented compounds in the essential oils yielded results that were partly consistent with those obtained by STRUCTURE and neighbour-joining analyses. However, their geographic distributions did not support the genetic grouping of the studied taxa and populations. The Mantel test showed a significant correlation between the phytochemical and genetic data (r = 0.421, p < 0.001). Genetic distance explained 17.8% of the phytochemical distance between populations. The current taxonomic position of several of the studied taxa is yet to be satisfactorily resolved, and further studies are needed. Such future research should include nuclear and plastid DNA sequences from a larger sample of populations and individuals.
Keywords: AFLP, Balkan peninsula, BAPS, essential oils, Mantel test, PCA, structure
Published in DiRROS: 08.03.2023; Views: 120; Downloads: 46
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Seasonal radial growth of Black pine (Pinus nigra Arnold) from Bosnia and Herzegovina, monitored by the pinning method and manual band dendrometersSimon Poljanšek
, Jernej Jevšenak
, Jožica Gričar
, Tom Levanič
, 2019, original scientific article
Abstract: Despite numerous dendroclimatological investigations into different tree species from Bosnia and Herzegovina, information is lacking on intra-annual wood formation patterns, which would help us to interpret the climate signal in tree rings better. Using the pinning method and manual band dendrometers, we investigated the seasonal dynamics of radial growth of black pine (Pinus nigra Arnold) trees in two successive growing seasons: 2011 and 2012. The up to 60-year-old trees grew in a stand at the base of a hill in the western, mountainous part of the Balkan Peninsula. The seasonal dynamics of wood formation and final number of cells differed between the studied years. Wood formation started in both years in early to mid-March. Differences were noticed in the wood production culmination; in 2011 it occurred at the end of May and beginning of June in 2012 and 2011, respectively. Xylem growth finished in 2012 in the middle of August and in 2011 in the middle of September. Based on the first derivative of the Gompertz function calculated rate of xylem growth was lowest in 2011. The dendrometers recorded a slow increment rate in spring, higher in summer and a decreasing rate again in the late summer in both growing seasons. In comparison with pinning, dendrometers showed a delay in the start of radial growth of up to 20 days in 2012. Additionally, dendrometers showed an increase in stem girth after the end of both growing seasons, when wood formation was already completed. Deviations between the two methods could be ascribed to the influence of water storage dynamics in the main stem and numerous structural processes in bark tissue, which are captured in dendrometer data. The influence of weather conditions on xylem phenology is also indicated by differences between the two studied years, although it is difficult to identify the influence of particular short-term weather events.
Keywords: pinning, manual dendrometers, radial growth, Pinus nigra, cambium, Balkan Peninsula
Published in DiRROS: 11.09.2019; Views: 4141; Downloads: 3606
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