1. Safety and efficacy of IL-12 plasmid DNA transfection into pig skin : supportive data for human clinical trials on gene therapy and vaccinationUrša Lampreht Tratar, Tanja Jesenko, Maša Omerzel, Alenka Seliškar, Urban Stupan, Mihajlo Djokić, Jerneja Sredenšek, Blaž Trotovšek, Gregor Serša, Maja Čemažar, 2024, original scientific article Abstract: Gene electrotransfer (GET) of plasmids encoding interleukin 12 (IL-12) has already been used for the treatment of various types of tumors in human oncology and as an adjuvant in DNA vaccines. In recent years, we have developed a plasmid encoding human IL-12 (phIL12) that is currently in a phase I clinical study. The aim was to confirm the results of a non-clinical study in mice on pharmacokinetic characteristics and safety in a porcine model that better resembled human skin. The GET of phIL12 in the skin was performed on nine pigs using different concentrations of plasmid phIL12 and invasive (needle) or noninvasive (plate) types of electrodes. The results of our study demonstrate that the GET of phIL-12 with needle electrodes induced the highest expression of IL-12 at the protein level on day 7 after the procedure. The plasmid was distributed to all tested organs; however, its amount decreased over time and was at a minimum 28 days after GET. Based on plasmid copy number and expression results, together with blood analysis, we showed that IL-12 GET is safe in a porcine animal model. Furthermore, we demonstrated that pigs are a valuable model for human gene therapy safety studies.
Keywords: interleukin 12, gene electrotransfer, immunotherapy
Published in DiRROS: 18.04.2024; Views: 17; Downloads: 10
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4. Kompoziti iz skorje: nova gozdarsko-lesarska vrednostna verigaSergej Medved, Grega Vilman, Urban Tajher, Mark Sever, Alan Antonović, 2023, original scientific article Abstract: Skorja je večopravilni (večfunkcijski) material, saj deluje kot zaščita (pred fizikalnimi in mehanskimi poškodbami, pa tudi pred biološko razgradnjo), omogoča transport vode in plinov, občasno pomaga pri fotosintezi in nudi podporo drevesu. Skorje je od 10 % do 20 % celotne mase drevesa, vendar je znaten delež ostane neizkoriščen. Da bi prikazali možnosti rabe skorje za izdelavo različnih kompozitov, smo izdelali in analizirali tri različne kompozite, in sicer lesno-plastični kompozit iz skorje in polietilena, trislojni ploščni kompozit iz skorje, zlepljene z melamin-urea-formaldehidnim lepilom, in enoslojni ploščni kompozit iz vlažnih iveri skorje, zlepljenih s taninskimi lepili.
Keywords: skorja, iveri, lesno plastični kompozit, iverna plošča
Published in DiRROS: 29.02.2024; Views: 170; Downloads: 38
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7. Evaluation of water equivalency of Plastic waterTM for high-energy electron beams using IAEA TRS-398 code of practiceBožidar Casar, Urban Zdešar, Vlado Robar, 2004, original scientific article Abstract: Electrochemotherapy (ECT) is a novel method for efficient tumor treatment in clinical environment. It combines local drug delivery and application of shorthigh voltage pulses, which permeabilize the plasma membrane by electroporation. Drug can enter only the cells with permeabilzed membrane. Recently, medical device CliniporatorTM for controlled electroporation was developed. Here, we present a web-application that extends the functionality of this medical device. The aim of the application is to collect, store and toallow the analysis of every ECT application using this medical device. The application helps transferring data collected by dev;ce during the electroporation process to the central database, and enables filling of medical records through the web forms. The application is based on technologies ASP, HTML, Flash, JavaScript, XML and others. The application main advantages are easy and rapid data access, scalability and independence of client computer operating system as well as easy application debugging and upgrading.
Published in DiRROS: 13.02.2024; Views: 119; Downloads: 26
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8. Small cellular particles from European spruce needle homogenateMarko Jeran, Anna Romolo, Vesna Spasovski, Matej Hočevar, Urban Novak, Roman Štukelj, Vid Šuštar, Matic Kisovec, Apolonija Bedina Zavec, Ksenija Kogej, Aleš Iglič, Polonca Trebše, Veronika Kralj-Iglič, 2023, original scientific article Keywords: Picea abies, spruce needles, extracellular particles, extracellular vesicles, small cellular particles, interferometric light microscopy, exosomes, cell-to-cell communication, drug delivery
Published in DiRROS: 07.02.2024; Views: 152; Downloads: 99
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