1. Biallelic RFC1 expansions are a rare cause of early-onset and familial Parkinson's diseaseAnja Kovanda, Lara Šušmelj, Helena Jaklič, Tadeja Lukežič, Aleš Maver, Igor N. Petrović, Borut Peterlin, 2026, other scientific articles Keywords: CANVAS, neurodegenerative disease, vestibular areflexia syndrome, CANVAS, RFC1, PCR
Published in DiRROS: 18.03.2026; Views: 168; Downloads: 129
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2. Minimum performance parameters to select tests for validation and selection of laboratories for TPS : grant agreement N. 773139, deliverable N° 1.1Špela Alič, Géraldine Anthoine, A Chabirand, Anne-Marie Chappé, Tanja Dreo, L Laurenson, Tadeja Lukežič, Nataša Mehle, H Mouaziz, Manca Pirc, Maja Ravnikar, Jenny Tomlinson, Marcel Westenberg, 2019, treatise, preliminary study, study Abstract: The aim of deliverable 1.1. is to prepare criteria to select tests for validation and to select laboratories for TPS (test performance study). Criteria for selection of tests for the TPS for each pest have been set (see Tables 7-12). These criteria have been divided in five groups: 1) validation data, 2) applicability, 3) protocols, 4) chemicals and 5) equipment. For selection of participants for the TPS selection criteria have also been set (see Table 13). Amongst the most important criteria for selection for participants of TPS are technical expertise for the pest group and the method, authorization to work with the specific pest and that the participating laboratory has quality assurance in place. These criteria enable evaluation of whether participants are proficient to perform the tests, have the necessary equipment and a permit to work with viable regulated organism. The scope of the testing for specific pests was set and common rules for each selection process was defined.
Keywords: pests, tests
Published in DiRROS: 03.09.2024; Views: 1076; Downloads: 1633
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3. List of tests for validation : round 1, grant agreement N. 773139Špela Alič, Géraldine Anthoine, A Chabirand, Anne-Marie Chappé, Tanja Dreo, Tjaša Jakomin, L Laurenson, Tadeja Lukežič, Nataša Mehle, E Metz-Verschure, H Mouaziz, J Oorspronk, Manca Pirc, Maja Ravnikar, Naomi te Braak, Jenny Tomlinson, Marcel Westenberg, 2019, treatise, preliminary study, study Abstract: We prepared a list of methods and tests for validation in test performance study (TPS) Round 1, both for laboratory and on-site use, for 6 selected pests: Erwinia amylovora, Pantoea stewartiisubsp. stewartii, citrus tristeza virus, plum pox virus, Fusarium circinatum and Bursaphelenchus xylophilus. The listed tests were first validated in preliminary studies by TPS organizers in order to select the final tests for TPS, based on the scope and criteria prepared in D1.1.
Keywords: pests, tests
Published in DiRROS: 03.09.2024; Views: 1251; Downloads: 614
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4. TPS reports with description of the method, materials and software used, as well as the data analysis - Round 2, Version 1.0 : grant agreement N. 773139Géraldine Anthoine, Ian Brittain, Aude Chabirand, Tanja Dreo, Francesco Faggioli, Catherine Harrison, Marta Luigi, Tadeja Lukežič, Slavica Matić, Nataša Mehle, Monica Mezzalama, Hanna Mouaziz, Maja Ravnikar, Jean-Philippe Renvoise, Davide Spadaro, Laura Tomassoli, Ana Vučurović, 2021, treatise, preliminary study, study Abstract: The aim of the deliverable 1.4. is to present a summary of the results obtained in the Round 1 of the test performance studies (TPS) organized by WP1 on six prioritized pests. Tests selection for each TPS was conducted following the “Common rules for selection of tests for TPS” and based on the “Weighted criteria for selection of tests for TPS”, both described in deliverable D1.1, while the list of selected tests for each TPS is available and explained in deliverable D1.2. TPS participants were selected following the “Common rules for selection of participants for TPS” and based on the “Criteria for selection of participants of TPS”, also both described in deliverable D1.1. For each of the six TPSs, the methodology used to perform the tests, the results of preliminary studies to select the tests, the results of the TPS and their thorough analysis and interpretation are described in corresponding TPS reports (supplementary information available upon request under confidentiality agreement). The validation data obtained during the six TPSs will be available in the validation section of the EPPO database on the diagnostic expertise. Main outcomes for each of the TPSs are highlighted as well as difficulties noticed during the organization process, which will improve organization of the following studies in the Round 2.
Keywords: test performance study, plant diseases
Published in DiRROS: 03.09.2024; Views: 1190; Downloads: 636
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5. Hydrodynamic cavitation efficiently inactivates potato virus Y in waterArijana Filipić, Tadeja Lukežič, Katarina Bačnik, Maja Ravnikar, Meta Ješelnik, Tamara Košir, Martin Petkovšek, Mojca Zupanc, Matevž Dular, Ion Gutiérrez-Aguirre, 2022, original scientific article Abstract: Waterborne plant viruses can destroy entire crops, leading not only to high financial losses but also to food shortages. Potato virus Y (PVY) is the most important potato viral pathogen that can also affect other valuable crops. Recently, it has been confirmed that this virus is capable of infecting host plants via water, emphasizing the relevance of using proper strategies to treat recycled water in order to prevent the spread of the infectious agents. Emerging environmentally friendly methods such as hydrodynamic cavitation (HC) provide a great alternative for treating recycled water used for irrigation. In the experiments conducted in this study, laboratory HC based on Venturi constriction with a sample volume of 1 L was used to treat water samples spiked with purified PVY virions. The ability of the virus to infect plants was abolished after 500 HC passes, corresponding to 50 min of treatment under pressure difference of 7 bar. In some cases, shorter treatments of 125 or 250 passes were also sufficient for virus inactivation. The HC treatment disrupted the integrity of viral particles, which also led to a minor damage of viral RNA. Reactive species, including singlet oxygen, hydroxyl radicals, and hydrogen peroxide, were not primarily responsible for PVY inactivation during HC treatment, suggesting that mechanical effects are likely the driving force of virus inactivation. This pioneering study, the first to investigate eukaryotic virus inactivation by HC, will inspire additional research in this field enabling further improvement of HC as a water decontamination technology.
Keywords: hydrodynamic cavitation, potato virus Y, virus inactivation, water decontamination
Published in DiRROS: 16.07.2024; Views: 1595; Downloads: 703
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