1. Vpliv sistemske terapije na cirkulirajoče tumorske celice in skupke cirkulirajočih tumorskih celicKlara Grad, Sara Šauer, Erika Matos, Domen Ribnikar, Nataša Snoj Šarvari, Tanja Ovčariček, Veronika Škrjanc, Živa Pišljar, Maja Čemažar, Simona Miceska, Tanja Jesenko, Veronika Kloboves-Prevodnik, Cvetka Grašič-Kuhar, 2025, published scientific conference contribution abstract Keywords: eksperimentalna onkologija, sistemska terapija, cirkulirajoče celice
Published in DiRROS: 18.03.2026; Views: 298; Downloads: 107
 Full text (105,17 KB) |
2. Vzpostavitev mišjega modela trojno negativnega raka dojk 4T1 za proučevanje cirkulirajočih tumorskih celic in procesa zasevanjaVeronika Škrjanc, Simona Kranjc Brezar, Maja Čemažar, Boštjan Markelc, Cvetka Grašič-Kuhar, Simona Miceska, Veronika Kloboves-Prevodnik, Tanja Jesenko, 2025, published scientific conference contribution abstract Keywords: citopatologija, diagnostična dejavnost, onkologija
Published in DiRROS: 18.03.2026; Views: 215; Downloads: 108
Full text (104,61 KB) |
3. Ovrednotenje cirkulirajočih tumorskih celic in celičnih skupkov pri bolnicah s primarnim, napredovalnim seroznim rakom jajčnika visokega gradusa pred neoadjuvantnim sistemskim zdravljenjemSimona Miceska, Tanja Jesenko, Nina Pišljar, Erik Škof, Cvetka Grašič-Kuhar, Veronika Kloboves-Prevodnik, 2025, published scientific conference contribution abstract Keywords: citopatologija, diagnostična dejavnost, onkologija
Published in DiRROS: 11.03.2026; Views: 247; Downloads: 119
Full text (104,64 KB) |
4. |
5. |
6. Ocena proliferacijskega indeksa Ki-67 v citoloških vzorcih nodalnih B-celičnih limfomovVeronika Kloboves-Prevodnik, Simona Miceska, Mojca Založnik, Simon Buček, Nataša Nolde, Mojca Gjidera, Ulrika Klopčič, Zorica Čekić, Živa Pohar-Marinšek, Gorana Gašljević, 2024, published scientific conference contribution abstract Keywords: limfomi, patologija, onkologija
Published in DiRROS: 25.02.2026; Views: 274; Downloads: 78
Full text (83,78 KB) |
7. Metode določevanja cirkulirajočih tumorskih celic raka dojke na Onkološkem inštitutu LjubljanaTanja Jesenko, Simona Miceska, Živa Pišljar, Maja Čemažar, Veronika Kloboves-Prevodnik, Cvetka Grašič-Kuhar, 2024, published scientific conference contribution Keywords: rak dojke, patologija, onkologija
Published in DiRROS: 25.02.2026; Views: 387; Downloads: 92
Full text (96,26 KB) |
8. Analiza cirkulirajočih tumorskih celic v krvi bolnic z napredovalim rakom jajčnika visokega gradusa ob diagnoziTanita Kranjc, Simona Miceska, Erik Škof, Maja Krajec, Brigita Gregorič, Breda Škrbinc, Veronika Kloboves-Prevodnik, 2024, published scientific conference contribution abstract Keywords: rak jajčnikov, eksperimentalna onkologija, onkologija
Published in DiRROS: 25.02.2026; Views: 339; Downloads: 100
Full text (84,75 KB) |
9. Association of tumor-infiltrating lymphocytes and inflammation status with survival outcome in patients with high-grade serous ovarian carcinomaSimona Miceska, Cvetka Grašič-Kuhar, Snježana Frković-Grazio, Erik Škof, Praveen Krishnamoorthy, Dineo Khabele, Veronika Kloboves-Prevodnik, 2025, original scientific article Abstract: In this study, we investigated the association between tumor-infiltrating lymphocytes (TILs), inflammation status, and progression-free survival (PFS) in patients with primary high-grade serous ovarian carcinoma (HGSC). We assessed the percentages of different intraepithelial and stromal TIL subtypes using both manual and digital methods, following established recommendations for TIL assessment. In addition, we evaluated inflammation status through several immune scores, including the pan-immune-inflammation value (PIV). Our results suggest that stromal CD3+ and CD8+ TILs, as well as PIV, may serve as potential prognostic indicators in HGSC, as they remained potential independent markers in multivariate analysis.
Keywords: ovarian carcinoma, high-grade serous ovarian carcinoma, prognostic factors
Published in DiRROS: 05.12.2025; Views: 973; Downloads: 165
 Link to file |
10. Complementary detection strategies for circulating tumor cells in breast cancer: clinical implications of combining immunofluorescence and cytopathological stainingTanja Jesenko, Cvetka Grašič-Kuhar, Živa Pišljar, Simona Miceska, Veronika Kloboves-Prevodnik, Maja Čemažar, 2025, original scientific article Abstract: Background: Circulating Tumor Cells (CTCs) serve as important biomarkers for disease monitoring and treatment response in patients with metastatic breast cancer. Their detection remains challenging because of their low abundance, phenotypic diversity and non-standardized mode of detection. Cytopathological Giemsa and Immunofluorescence (IF) staining can offer complementary approaches for CTC characterization. Giemsa staining enables assessment of cellular morphology, while IF allows for marker-specific identification, together providing a more comprehensive and accurate evaluation of CTCs. Methods: We developed an IF staining protocol with antibodies against Cytokeratin (CK), vimentin (VIM), and Cluster of Differentiation 45 (CD45) to distinguish epithelial, mesenchymal, hybrid and hematopoietic cells for CTC detection and characterization and compared it with cytopathologic method of detection via Giemsa staining with regard to CTC detection rates and morphological detail. Results: Study was performed on the samples of 29 heavily pretreated patients with metastatic breast cancer (median duration of metastatic disease 19.4 months). Giemsa staining enabled the detection of a higher number of CTCs compared to our IF protocol. Lower detection rate was potentially due to the loss of fragile or loosely adherent cells during methanol fixation and IF staining. Additionally, in IF-stained samples, some CTCs presented faint nuclear signals, potentially impairing their recognition. The IF staining supported the identity of CTCs detected on Giemsa-stained slides by employing a three-color antibody panel-based approach and allowed detailed phenotypic discrimination and structural analysis of CTCs, including the identification of a distinctive CK polarization pattern suggestive of a transitional state during intravasation. Conclusion: Giemsa and IF may thus be complementary rather than mutually exclusive and relying on a single detection approach could underestimate the true CTC burden. An integrative strategy combining both techniques may offer a more comprehensive view of CTC populations in metastatic breast cancer, thereby enhancing diagnostic precision.
Keywords: biomarkers, breast cancer, circulating tumor cells, cytopathological detection
Published in DiRROS: 05.12.2025; Views: 1074; Downloads: 192
Link to file |
