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Query: "author" (Simona Kranjc) .

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1.
Razvoj in karakterizacija mišjega modela za študije HPV-pozitivnega raka glave in vratu
Živa Modic, Maja Čemažar, Boštjan Markelc, Andrej Cör, Gregor Serša, Simona Kranjc Brezar, Tanja Jesenko, 2023, published scientific conference contribution abstract

Keywords: miši, rak glave in vratu, gensko zdravljenje
Published in DiRROS: 19.06.2023; Views: 168; Downloads: 59
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Imunoterapija z bakteriofagi, ki na plaščnih proteinih predstavljajo tumorski peptid MAGE-A1 za zdravljenje malignega melanoma
Nuša Brišar, Katja Šuster, Simona Kranjc Brezar, Andrej Cör, 2023, published scientific conference contribution abstract

Keywords: imunoterapija, melanom, bakteriofagi
Published in DiRROS: 19.06.2023; Views: 146; Downloads: 54
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Obsevanje in genska terapija s kemokinoma CCL5 ali CCL17 na mišjih modelih karcinoma
Tim Božič, Simona Kranjc Brezar, Boštjan Markelc, Maja Čemažar, 2023, published scientific conference contribution abstract

Keywords: miši, obsevanje, gensko zdravljenje
Published in DiRROS: 19.06.2023; Views: 124; Downloads: 47
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Novosti na področju radioterapije in radiobiologije: od raziskav do klinike : zbornik prispevkov in povzetkov
2023, proceedings of peer-reviewed scientific conference contributions (international and foreign conferences)

Published in DiRROS: 09.06.2023; Views: 143; Downloads: 75
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Gene electrotransfer of IL-2 and IL-12 plasmids effectively eradicated murine B16.F10 melanoma
Tilen Komel, Maša Bošnjak, Simona Kranjc Brezar, Mariangela De Robertis, M. Mastrodonato, G. Scillitani, G. Pesole, Emanuella Signori, Gregor Serša, Maja Čemažar, 2021, original scientific article

Abstract: Gene therapy has become an important approach for treating cancer, and electroporation represents a technology for introducing therapeutic genes into a cell. An example of cancer gene therapy relying on gene electrotransfer is the use of immunomodulatory cytokines, such as interleukin 2 (IL-2) and 12 (IL-12), which directly stimulate immune cells at the tumour site. The aim of our study was to determine the effects of gene electrotransfer with two plasmids encoding IL-2 and IL-12 in vitro and in vivo. Two different pulse protocols, known as EP1 (600 V/cm, 5 ms, 1 Hz, 8 pulses) and EP2 (1300 V/cm, 100 %s, 1 Hz, 8 pulses), were assessed in vitro for application in subsequent in vivo experiments. In the in vivo experiment, gene electrotransfer of pIL-2 and pIL-12 using the EP1 protocol was performed in B16.F10 murine melanoma. Combined treatment of tumours using pIL2 and pIL12 induced significant tumour growth delay and 71% complete tumour regression. Furthermore, in tumours coexpressing IL-2 and IL-12, increased accumulation of dendritic cells and M1 macrophages was obtained along with the activation of proinflammatory signals, resulting in CD4 + and CD8 + T-lymphocyte recruitment and immune memory development in the mice. In conclusion, we demonstrated high antitumour efficacy of combined IL-2 and IL-12 gene electrotransfer protocols in low-immunogenicity murine B16.F10 melanoma.
Keywords: gene therapy, gene electrotransfer, IL-12, immunotherapy, melanoma
Published in DiRROS: 23.09.2022; Views: 331; Downloads: 110
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Gene electrotransfer of proinflammatory chemokines CCL5 and CCL17 as a novel approach of modifying cytokine expression profile in the tumor microenvironment
Tim Božič, Gregor Serša, Simona Kranjc Brezar, Maja Čemažar, Boštjan Markelc, 2021, original scientific article

Abstract: The effectiveness of immunotherapy highly correlates with the degree and the type of infiltrated immune cells in the tumor tissue. Treatments based on modifying the immune cell infiltrate of the tumor microenvironment are thus gaining momentum. Therefore, the aim of our study was to investigate the effects of gene therapy with two proinflammatory chemokines CCL5 and CCL17 on inflammatory cytokine expression profile and immune cell infiltrate in two murine breast tumor models, 4T1 and E0771, and two murine colon tumor models, CT26 and MC38. In vitro, lipofection of plasmid DNA encoding CCL5 or CCL17 resulted in changes in the cytokine expression profile similar to control plasmid DNA, implying that the main driver of these changes was the entry of foreign DNA into the cell%s cytosol. In vivo, gene electrotransfer resulted in high expression levels of both Ccl5 and Ccl17 transgenes in the 4T1 and CT26 tumor models. Besides a minor increase in the survival of the treated mice, the therapy also resulted in increased expression of Cxcl9 and Ifn%, potent activators of the immune system, in CT26 tumors. However, this was not recapitulated in changes of TME, implying that a further refinement of the dosing schedule is needed.
Keywords: chemokines, cytokine expression, gene electrotransfer, CCL5
Published in DiRROS: 19.09.2022; Views: 301; Downloads: 90
.pdf Full text (5,63 MB)

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