1. Cadmium in potato crops : differences among varietiesEva Smolnikar, Peter Dolničar, Kristina Ugrinović, 2026, published scientific conference contribution Keywords: cadmium, potato, potato vaeieties, potato crops, cd intake, environmental conditions
Published in DiRROS: 29.06.2026; Views: 263; Downloads: 100
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2. Optimised extraction and HPLC–DAD determination of steroidal glycoalkaloids in potato tubers : analytical validation and environmental sustainability evaluationHajer Ben Ammar, Peter Dolničar, Barbara Pipan, Lovro Sinkovič, 2026, original scientific article Abstract: Steroidal glycoalkaloids ( α-solanine and α-chaconine) are major potato toxins whose accurate quantification is often challenged by matrix effects and chromatographic selectivity. This study reports the optimisation and validation of a robust reversed-phase HPLC-DAD method for the determination of glycoalkaloids in potato tissues, combined with a systematic assessment of the environmental sustainability of the analytical workflow. Extraction conditions were optimized by integrating systematic solvent screening with a factorial experimental design to evaluate the effect of key operational variables on glycoalkaloid recovery and refine extraction performance The resulting extraction medium improved extract cleanliness and chromatographic stability. A subsequent C18 solid-phase extraction SPE step further reduced matrix effect and enhanced analytical selectivity. Chromatographic separation was achieved on a conventional C18 column using an acetonitrile-water gradient, providing baseline resolution (Rs > 1.5) of α 2 -solanine and α-chaconine with stable diode-array detection (DAD) at 205 nm. The method was validated according to ICH Q2(R2) and AOAC guidelines, demonstrating excellent linearity (R > 0.995), low limits of detection (0.462–0.767 µg/mL) and quantification (1.540–2.558 µg/mL), high precision (≤ 5% RSD), and recoveries of 90–105%. Robustness testing confirmed minimal sensitivity to routine variations in chromatographic parameters. Application to potato breeding lines demonstrated reliable quantification and revealed genotype-dependent variation in glycoalkaloid content. The environmental assessment of the analytical procedure was evaluated using Analytical Eco-scale, GAPI and AGREE metrics, demonstrating reduced solvent consumption, avoidance of strongly acidic extraction conditions, and a simplified sample preparation workflow. The proposed HPLC-DAD workflow provides a validated, high-performance, and environmentally conscious approach for routine GA quantification, suitable for food-safety monitoring, regulatory compliance and breeding programme applications.
Keywords: α-chaconine, α-solanine, analytical method, HPLC-DAD, potato tubers, environmental sustainability
Published in DiRROS: 10.06.2026; Views: 270; Downloads: 277
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3. Uncovering phenotypic variation in common bean (Phaseolus vulgaris L.) : insights from the INCREASE projectHourieh Tavakoli Hasanaklou, Lovro Sinkovič, Roberto Papa, Elena Bitocchi, Elisa Bellucci, Peter Dolničar, Barbara Pipan, 2026, original scientific article Abstract: The common bean (Phaseolus vulgaris L.) is a major food legume and an important plant genetic resource for sustainable agriculture. Effective use of this diversity requires integrated evaluation of phenotypic variation and agronomic performance, with preliminary assessments of line performance across seasons. In this study, phenotypic diversity was evaluated in a subsample of the INCREASE R-core collection, a large and well-defined core set of common-bean SSD lines derived from heterogeneous germplasm lines. A total of 507 lines were characterized using 57 agro-morphological traits. Multivariate analyses revealed wide phenotypic diversity structured mainly by growth habit, phenology, and yield-related traits, with clear differentiation among lines. Mixed-data clustering identified cluster 4 as the main phenotypic group associated with higher seed- and yield-related performance and composed predominantly of indeterminate climbing landraces. Multi-trait selection indices generally ranked lines from this group highest, while early, small-seeded types tended to show lower overall performance. Evaluation of a selected subset of 19 lines across two growing seasons revealed marked year-to-year variation in yield performance, indicating contrasting responses among otherwise high-performing lines. The multi-trait genotype–ideotype distance index further distinguished lines with balanced performance across traits and years. Overall, this study shows that large-scale phenotypic characterization combined with multi-trait evaluation can provide a useful exploratory basis for identifying breeding-relevant ideotypes and promising lines for further validation for common-bean improvement.
Keywords: common bean, phenotypic diversity, germplasm evaluation, multivariate analysis, selection index, year-to-year variation, yield stability
Published in DiRROS: 20.04.2026; Views: 461; Downloads: 220
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4. Glycoalkaloids in potato : a comprehensive overview of accumulation, detection and mitigation strategiesPeter Dolničar, Lovro Sinkovič, 2025, original scientific article Keywords: potato, α-Chaconine, α-Solanine, analytical methods, detection sensitivity, fod safety
Published in DiRROS: 12.11.2025; Views: 1068; Downloads: 468
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5. Combining multi-criteria decision analysis with agro-morphological-biochemical-molecular traits of interest for use in breeding in promising common bean breeding lines (Phaseolus vulgaris L.)Lovro Sinkovič, Hourieh Tavakoli Hasanaklou, Mohamed Neji, Eva Plestenjak, Peter Dolničar, Vladimir Meglič, Barbara Pipan, 2024, original scientific article Keywords: Breeding line, multivariateanalysis, quantitative trait, qualitative trait, SCarmarker, trypsin inhibitors
Published in DiRROS: 18.12.2024; Views: 1433; Downloads: 819
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6. Field-scale UAV-based multispectral phenomics: : Leveraging machine learning, explainable AI, and hybrid feature engineering for enhancements in potato phenotypingJanez Lapajne, Andrej Vončina, Ana Vojnović, Daša Donša, Peter Dolničar, Uroš Žibrat, 2025, original scientific article Keywords: Multispectral imaging, Potato research, Machine learning, Interpretability techniques
Published in DiRROS: 10.12.2024; Views: 1539; Downloads: 889
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7. Late blight resistance conferred by Rpi-Smira2/R8 in potato genotypes in vitro depends on the genetic backgroundEva Blatnik, Marinka Horvat, Sabina Berne, Miha Humar, Peter Dolničar, Vladimir Meglič, 2022, original scientific article Keywords: potato, potato genotype, potato R8 genotypes, in vitro infection, late blight, resistant cultivars, oomicete, Sárpo Mira
Published in DiRROS: 01.09.2022; Views: 2019; Downloads: 804
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8. Opisna sortna lista za krompir 2015Peter Dolničar, 2015, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: krompir, sorte, sortne liste, Slovenija
Published in DiRROS: 11.07.2017; Views: 6822; Downloads: 2626
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9. Svetovalni kodeks dobre kmetijske prakse :
varovanje voda, tal, zraka in ohranjanje biotske raznovrstnostiJože Verbič, Janez Sušin, Andrej Simončič, Zoran Čergan, Drago Babnik, Viktor Jejčič, Tomaž Poje, Matej Knapič, Janko Verbič, Peter Dolničar, Dušica Majer, Kristina Ugrinović, Robert Janža, Jože Maljevič, Matej Stopar, Andrej Zemljič, 2006, professional monograph Keywords: kmetijska praksa, dobra kmetijska praksa, varovanje voda, varovanje tal, varovanje zraka, biotska raznovrstnost
Published in DiRROS: 11.07.2017; Views: 8313; Downloads: 2853
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10. V sortno listo Republike Slovenije se vpiše sorta krompirja (Solanum tuberosum L.), z odobrenim imenom KIS Kokra : registrska številka sorte SOT194 : odločba RS Ministrstva za kmetijstvo, gozdarstvo in prehrano, Fitosanitarne uprave RS, 3432-11/2006/6, 20.7.2010Peter Dolničar, Jelka Šuštar Vozlič, Vladimir Meglič, 2010, new variety Keywords: krompir, kultivarji, Solanum tuberosum, cv. Kokra, nove sorte
Published in DiRROS: 11.07.2017; Views: 11359; Downloads: 2419
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