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Query: "author" (Marjana Regvar) .

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1.
When "biodegradable" is not benign: Microplastic-driven disruption of soil processes and plant-microbe interactions
Teja Pelko, Anita Jemec Kokalj, Marjana Regvar, Marina Dermastia, Katarina Vogel-Mikuš, 2026, review article

Abstract: The increasing use of biodegradable plastics (BPs) as alternatives to conventional plastics (CPs) is leading to the accumulation of biodegradable microplastics (BMPs) in terrestrial environments. Contrary to assumptions of rapid degradation, BMPs can persist in soil long enough to interact with key biological processes. This review advances the field by proposing a mechanistic framework linking BMP aging and degradation, soil physicochemical transformations, plastisphere assembly, rhizosphere interactions, and plant responses, and by critically evaluating the sources of inconsistency across studies. We show that divergent effects of BMPs can be best explained by four interacting determinants: polymer chemistry and additive composition, aging-driven surface transformations, soil physicochemical properties, and rhizosphere processes including plant-mediated effects. Through these coupled pathways, BMPs can alter aggregation, pore architecture, pH, enzyme activity, and carbon and nutrient cycling, thereby reshaping the soil environment in which microorganisms and roots interact. BMP surfaces can also act as dynamic microbial niches that promote biofilm formation, shift microbial community composition and function, and under certain conditions may facilitate pollutant transport, pathogen persistence, and horizontal gene transfer. Plant responses to BMPs are predominantly indirect and emerge from rhizosphere-mediated processes, which helps explain the wide variability in reported plant responses, ranging from subtle metabolic changes to pronounced growth inhibition. However, current evidence is constrained by short-term studies and insufficient consideration of aged materials. Biodegradability should therefore not be equated with low ecological risk in soils. Progress in this field requires integrative approaches linking BMP properties, plastisphere dynamics, and plant–soil interactions over time.
Keywords: soil ecosystem, biodegradable microplastics, plant microbiome, plastisphere, rhizosphere, nutrient cycling
Published in DiRROS: 06.05.2026; Views: 392; Downloads: 347
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2.
The potential of Si and Se as biostimulants to enhance resistance to climatic conditions and improve yields in common and Tartary buckwheat
Mateja Germ, Aleksandra Golob, Katarina Vogel Mikuš, Matevž Likar, Jure Mravlje, Paula Pongrac, Anja Mavrič Čermelj, Cheol Ho Park, Min Ook Park, Jacek Kwiatkowski, Marjana Regvar, 2025, original scientific article

Abstract: Common buckwheat and Tartary buckwheat are pseudocereals and grow worldwide. Due to the high concentration of flavonoids, buckwheats are potential sources of smart food. Tartary and common buckwheat are traditionally grown in mountain regions of China, Korea, the northern parts of India, Bhutan, and Nepal. Plants that grow in high elevations are exposed to intense UV radiation, which can harm susceptible sites in the plants. Plants defend themselves against intense radiation by synthesising UV-absorbing compounds. Drought will probably become more frequent and intense due to climate change. UV radiation and drought are environmental parameters that present stress to the plants. These impacts can be synergistic or antagonistic. Selenium (Se) and silicon (Si) can protect plants exposed to UV radiation or drought since Se acts as an antioxidant. Silicon is an abundant element in Earth’s crust. It is present as a liquid or an amorphous or crystalline solid phase in the soil. Selenium and silicon are not essential elements for vascular plants, but they may positively affect plants. Thus, they can be added to the growth media to improve crop yield and quality, enhance resistance to  biotic and biotic stress and improve plant growth.
Keywords: common buckwheat, Tartary buckwheat, elements, UV radiations, drought
Published in DiRROS: 07.01.2026; Views: 604; Downloads: 413
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Correlative imaging of structural biochemistry in plant and food quality research within an interoperable data acquisition platform
Marjana Regvar, Boštjan Jenčič, Martin Šala, Aleš Kladnik, Iztok Dogša, Maja Koblar, Mitja Kelemen, Primož Vavpetič, Katarina Vogel-Mikuš, Ivan Kreft, Primož Pelicon, Paula Pongrac, 2025, original scientific article

Abstract: Correlative imaging is a powerful tool for revealing information on cell-type structures and their biochemistry, with the potential to inform healthier food choices and improved dietary recommendations. Determination of plant structures and their structural biochemistry advances our understanding of specific structures designed to store different biomolecules within cells and tissues. Compared to the classical biochemical separation techniques, the key advantage of sequential correlative imaging techniques is in relating spatial plant (micro)structures to their biochemistry in a nondestructive manner. Sequential imaging reported here comprises six methodologies on a single sample, a cross-section of a Tartary buckwheat (Fagopyrum tataricum) grain, namely, bright-field and autofluorescence microscopy, fluorescence microspectroscopy, MeV-secondary ion mass spectrometry, micro-particle-induced X-ray emission, scanning electron microscopy coupled with energy dispersive X-ray spectroscopy, and laser ablation-inductively coupled plasma-mass spectrometry. Results confirm that the stepwise addition of the desired information across several classes of biomolecules and several spatial scales informs the quality and safety of plant-based produce across scales. Therefore, a viable workflow is proposed, enabling sequential spatial analysis of grain and highlighting plant structures' in situ specificity. The advantages and disadvantages of the selected methodologies were critically evaluated.
Keywords: autofluorescence, correlative molecular imaging, element distribution, grain tissues
Published in DiRROS: 07.11.2025; Views: 640; Downloads: 489
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Nodulation performance and agronomic traits of European common bean (Phaseolus vulgaris L.) genetic resources
Eva Plestenjak, Vladimir Meglič, Lovro Sinkovič, Matevž Likar, Marjana Regvar, Barbara Pipan, 2024, original scientific article

Abstract: Field production of common beans benefit from root microbial associations, although they are generally considered to be weaker nitrogen fixers than other legumes. Therefore, the number of nodules on the roots of 64 accessions of the European common bean (Phaseolus vulgaris L.) and the relationship between the number of nodules per accession and other plant characteristics (growth type, earliness, seed yield, and total nitrogen content in seeds) were studied. The results indicated that growth type and earliness influenced the number of nodules per accession (the contributions of principal component analysis were 34.9% for Dim1 and 29.8% for Dim2). The average number of nodules per accession with indeterminate growth type was almost five times higher (20.1) than for accessions with determinate growth type (4.4). Common bean accessions with regular growth cycle length had the highest number of nodules (21.9). In contrast, nodulation efficiency, measured as seed yield per plant and total nitrogen content in seeds, were not correlated with the number of nodules per accession (correlation analysis, r < 0.1). Consequently, data on the nodulation efficiency of European common bean accessions are important for breeding programmes in conjunction with other agronomically important traits for commercial and/or organic cultivation systems.
Keywords: agronomic traits, common bean, nitrogen fixation, nodules, symbiotic efficiency
Published in DiRROS: 30.09.2024; Views: 1832; Downloads: 474
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