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2. Engineered combinatorial cell device for wound healing and bone regenerationLucija Kadunc, Duško Lainšček, Rok Gašperšič, Petra Sušjan, Uroš Kovačič, Miha Butinar, Boris Turk, Roman Jerala, Iva Hafner Bratkovič, 2023, original scientific article Abstract: Growth factors are the key regulators that promote tissue regeneration and healing processes. While the effects of individual growth factors are well documented, a combination of multiple secreted growth factors underlies stem cell–mediated regeneration. To avoid the potential dangers and laborintensive individual approach of stem cell therapy while maintaining their regeneration-promoting effects based on multiple secreted growth factors, we engineered a “mix-and-match” combinatorial platform based on a library of cell lines producing growth factors. Treatment with a combination of growth factors secreted by engineered mammalian cells was more efficient than with individual growth factors or even stem cell–conditioned medium in a gap closure assay. Furthermore, we implemented in a mouse model a device for allogenic cell therapy for an in situ production of growth factors, where it improved cutaneous wound healing. Augmented bone regeneration was achieved on calvarial bone defects in rats treated with a cell device secreting IGF, FGF, PDGF, TGF-β, and VEGF. In both in vivo models, the systemic concentration of secreted factors was negligible, demonstrating the local effect of the regeneration device. Finally, we introduced a genetic switch that enables temporal control over combinations of trophic factors released at different stages of regeneration mimicking the maturation of natural wound healing to improve therapy and prevent scar formation.
Keywords: wound healing, bone regeneration, growth factors
Published in DiRROS: 01.06.2023; Views: 288; Downloads: 107
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4. Selective inhibition of NLRP3 inflammasome by designed peptide originating from ASCPetra Sušjan, Duško Lainšček, Žiga Strmšek, Vesna Hodnik, Gregor Anderluh, Iva Hafner Bratkovič, 2020, original scientific article Keywords: blood-brain barrier, inflammation, ▫$IL-1beta$▫, peptide inhibitors
Published in DiRROS: 24.03.2022; Views: 519; Downloads: 344
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5. Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivoAjasja Ljubetič, Fabio Lapenta, Helena Gradišar, Igor Drobnak, Jana Aupič, Žiga Strmšek, Duško Lainšček, Iva Hafner Bratkovič, Andreja Majerle, Nuša Krivec, Mojca Benčina, Tomaž Pisanski, Tanja Ćirković-Veličković, Adam Round, José María Carazo, Roberto Melero, Roman Jerala, 2017, original scientific article Published in DiRROS: 17.04.2018; Views: 3648; Downloads: 2355
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6. Self-assembled structures composed of single polypeptide comprising at least three coiled-coil forming elements : patentRoman Jerala, Ota Fekonja, Jelka Pohar, Helena Gradišar, Mojca Benčina, Iva Hafner Bratkovič, Robert Bremšak, Špela Miklavič, Urška Jelerčič, Anja Lukan, Tibor Doles, Sabina Božič Abram, Marko Verce, Nika Debeljak, Jožica Friedrich, 2014, patent Keywords: self assembled structures, polipeptide chain, nenostructures
Published in DiRROS: 13.05.2015; Views: 5921; Downloads: 830
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