1. Clonality analysis of lymphoid proliferations using the BIOMED-2 clonality assays : a single institution experienceIra Koković, Barbara Jezeršek Novaković, Petra Škerl, Srdjan Novaković, 2014, original scientific article Abstract: Background. Clonality determination in patients with lymphoproliferative disorders can improve the final diagnosis.The aim of our study was to evaluate the applicative value of standardized BIOMED-2 gene clonality assay protocolsfor the analysis of clonality of lymphocytes in a group of different lymphoid proliferations.Materials and methods. With this purpose, 121 specimens from 91 patients with suspected lymphoproliferationssubmitted for routine diagnostics from January to December 2011 were retrospectively analyzed. According to thefinal diagnosis, our series comprised 32 cases of B-cell lymphomas, 38 cases of non-Hodgkins T-cell lymphomas and51 cases of reactive lymphoid proliferations. Clonality testing was performed using the BIOMED-2 clonality assays.Results. The determined sensitivity of the TCR assay was 91.9%, while the sensitivity of the IGH assay was 74.2%. Thedetermined specificity of the IGH assay was 73.3% in the group of lymphomas and 87.2% in the group of reactivelesions. The determined specificity of the TCR assay was 62.5% in the group of lymphomas and 54.3% in the group ofreactive lesions.Conclusions. In the present study, we confirmed the utility of standardized BIOMED-2 clonality assays for the detectionof clonality in a routine diagnostical setting of non-Hodgkins lymphomas. Reactions for the detection of thecomplete IGH rearrangements and reactions for the detection of the TCR rearrangements are a good choice forclonality testing of a wide range of lymphoid proliferations and specimen types while the reactions for the detectionof incomplete IGH rearrangements have not shown any additional diagnostic value.
Keywords: Biomed-2, clonality analysis, lymphomas, IGH rearrangement, TCR rearrangement
Published in DiRROS: 11.04.2024; Views: 55; Downloads: 17
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2. Clonality analysis of lymphoid proliferations using BIOMED-2 clonality assays : a single institution experienceIra Kokovič, Barbara Jezeršek Novaković, Petra Škerl, Srdjan Novaković, 2014, professional article Keywords: onkologija, rak (medicina), limfom, diagnostika, analiza, molekularne metode, molekularna diagnostika
Published in DiRROS: 10.04.2024; Views: 66; Downloads: 14
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4. Določanje klonalnosti limfoidnih proliferacij : uvedba nove metode in primerjava z dosedanjo metodoPetra Škerl, Ira Koković, Veronika Kloboves-Prevodnik, Srdjan Novaković, 2009, professional article Abstract: Na Oddelku za molekularno diagnostiko smo na področju diagnostike limfomov uvedli dodatno metodo za določanje klonalnosti limfoidnih proliferacij. Tudi nova metoda temelji na verižni reakciji s polimerazo (PCR) in na uporabi različnih konsenzusnih oligonukleotidnih začetnikov. Metoda je validirana in registrirana za diagnostične potrebe (oznaka CE). Z razširjenim naborom oligonukleotidnih začetnikov in uporabo večjega števila začetnikov smo, po naših izračunih, izboljšali občutljivost določanja klonalnosti limfocitov B za 23,1 %, določanje klonalnosti limfocitov T pa za 14,3 %. Teoretično lahko sedaj z obema metodama določimo monoklonalno populacijo limfocitov pri 84,6 % B- in 85,7 % T-limfomov
Published in DiRROS: 31.08.2018; Views: 3330; Downloads: 827
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5. Molekularna diagnostika limfomovIra Koković, Srdjan Novaković, 2008, professional article Abstract: Določanje klonalnosti limfoidnih proliferacij in dokazovanje specifičnih genetskih sprememb ima pomembno vlogo v diagnostiki limfoidnih neoplazem, saj omogoča ločevanje med neoplastičnimi lezijami in reaktivnimi procesi. Klonalno populacijo limfoidnih celic lahko dokažemo s pomnoževanjem preurejenih genov, ki kodirajo polipeptidne verige receptorjev na površini celic B in T. Z metodo PCR lahko dokažemo tudi kromosomske translokacije, značilne za posamezne vrste limfomov. Uvedli smo tehnike za določanje klonalnosti limfoidnih proliferacij in dokazovanje kromosomskih translokacij t(11;14) pri plaščnoceličnem ter t(14;18) pri folikularnem limfomu, ki temeljijo na polimerazni verižni reakciji. Tehnike so preproste, hitre in uporabne za molekularnobiološko preiskavo široke palete vzorcev. Specifičnost uvedenih metod je primerljiva s podatki v literaturi. Molekularnobiološke preiskave uporabljamo v diagnostiki limfomov od leta 1997.
Published in DiRROS: 31.08.2018; Views: 2957; Downloads: 702
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