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4. Recombinant human erythropoietin alters gene expression and stimulates proliferation of MCF-7 breast cancer cellsNina Trošt, Tina Stepišnik, Sabina Berne, Anja Pucer Janež, Toni Petan, Radovan Komel, Nataša Debeljak, 2013, published scientific conference contribution Abstract: Background. Functional erythropoietin (EPO) signaling is not specific only to erythroid lineages and has been confirmed in several solid tumors, including breast. Three different isoforms of erythropoietin receptor (EPOR) have been reported, the soluble (EPOR-S) and truncated (EPOR-T) forms acting antagonistically to the functional EPOR. In this study, we investigated the effect of human recombinant erythropoietin (rHuEPO) on cell proliferation, early gene response and the expression of EPOR isoforms in the MCF-7 breast cancer cell line.Materials and methods. The MCF-7 cells were cultured with or without rHuEPO for 72 h or 10 weeks and assessed for their growth characteristics, expression of early response genes and different EPOR isoforms. The expression profile of EPOR and EPOR-T was determined in a range of breast cancer cell lines and compared with their invasive properties.Results. MCF-7 cell proliferation after rHuEPO treatment was dependent on the time of treatment and the concentration used. High rHuEPO concentrations (40 U/ml) stimulated cell proliferation independently of a preceding long-term exposure of MCF-7 cells to rHuEPO, while lower concentrations increased MCF-7 proliferation only after 10 weeks of treatment. Gene expression analysis showed activation of EGR1 and FOS, confirming the functionality of EPOR. rHuEPO treatment also slightly increased the expression of the functional EPOR isoform, which, however, persisted throughout the 10 weeks of treatment. The expression levels of EPOR-T were not influenced. There were no correlations between EPOR expression and the invasiveness of MCF-7, MDA-MB-231, Hs578T, Hs578Bst, SKBR3, T-47D and MCF-10A cell lines.Conclusions. rHuEPO modulates MCF-7 cell proliferation in time- and concentration-dependent manner. We confirmed EGR1, FOS and EPOR as transcription targets of the EPO-EPOR signaling loop, but could not correlate the expression of different EPOR isoforms with the invasiveness of breast cancer cell lines.
Keywords: breast cancer, erythropoietin, gene expression
Published in DiRROS: 22.03.2024; Views: 99; Downloads: 40
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6. Metal–support interaction between titanium oxynitride and Pt nanoparticles enables efficient low-Pt-loaded high-performance electrodes at relevant oxygen reduction reaction current densitiesArmin Hrnjić, Ana Rebeka Kamšek, Lazar Bijelić, Anja Logar, Nik Maselj, Milutin Smiljanić, Jan Trputec, Natan Vovk, Luka Pavko, Francisco Ruiz-Zepeda, Marjan Bele, Primož Jovanovič, Nejc Hodnik, 2024, original scientific article Published in DiRROS: 04.03.2024; Views: 109; Downloads: 49
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9. Fine particulate matter (PM2.5) exposure assessment among active daily commuters to induce behaviour change to reduce air pollutionAnja Ilenič, Alenka Mauko Pranjić, Nina Zupančič, Radmila Milačič, Janez Ščančar, 2023, original scientific article Abstract: Fine particulate matter (PM2.5), a detrimental urban air pollutant primarily emitted by traffic and biomass burning, poses disproportionately significant health risks at relatively limited exposure during commuting. Previous studies have mainly focused on fixed locations when assessing PM2.5 exposure, while neglecting pedestrians and cyclists, who often experience higher pollution levels. In response, this research aimed to independently validate the effectiveness of bicycle-mounted low-cost sensors (LCS) adopted by citizens, evaluate temporal and spatial PM2.5 exposure, and assess associated health risks in Ljubljana, Slovenia. The LCS quality assurance results, verified by co-location field tests by air quality monitoring stations (AQMS), showed comparable outcomes with an average percentage difference of 21.29 %, attributed to humidity-induced nucleation effects. The colder months exhibited the highest air pollution levels (μ = 32.31 μg/m3) due to frequent thermal inversions and weak wind circulation, hindering vertical air mixing and the adequate dispersion of pollutants. Additionally, PM2.5 levels in all sampling periods were lowest in the afternoon (μ = 12.09 μg/m3) and highest during the night (μ = 61.00 μg/m3) when the planetary boundary layer thins, leading to the trapping of pollutants near the surface, thus significantly affecting diurnal and seasonal patterns. Analysis of exposure factors revealed that cyclists were approximately three times more exposed than pedestrians. However, the toxicological risk assessment indicated a minimal potential risk of PM2.5 exposure. The collaborative integration of data from official AQMS and LCS can enhance evidence-based policy-making processes and facilitates the realignment of effective regulatory frameworks to reduce urban air pollution.
Keywords: air pollution monitoring, PM2.5 exposure, citizen science, bicycle-mounted low-cost sensors
Published in DiRROS: 30.01.2024; Views: 172; Downloads: 66
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10. Surovinske potrebe za zagotavljanje prehoda na trajnostno in pametno mobilnost : zbornik povzetkov konferenčnih prispevkov2022, other monographs and other completed works Abstract: EIT Urbana mobilnost RIS Hub Slovenija, koordinirana s strani Zavoda za gradbeništvo Slovenije, deluje kot nacionalna kontaktna točka za skupnost urbana mobilnost, ki deluje pod okriljem Evropskega inštituta za inovacije in tehnologijo. Osredotočenost na izboljševanje življenjskih razmer v mestih, spodbujanje inovacij na področju zelenih in varnih rešitev za mobilnost ljudi, blaga in odpadkov, pospeševanje tržnih priložnosti v avtomobilski industriji ter oblikovanje zakonodajnega okvira za spremembe so le nekateri strateški cilji skupnosti KIC EIT Urbana mobilnost. Z namenom spodbujanja povezovanja, združevanja in povečevanja partnerstva med obema sektorjema (surovine in mobilnost), neposredno vpletenima v prehod na bolj trajnostne oblike mobilnosti, je bila 24. oktobra 2022 organizirana spletna konferenca EIT OPEN DAY SLOVENIJA2022 – Surovinske potrebe za zagotavljanje prehoda na trajnostno in pametno mobilnost. Konferenca je bila organizirana s strani Zavoda za gradbeništvo Slovenije, v sodelovanju z Regionalnim centrom Adria EIT Surovine RIS Hub in Javno agencijo SPIRIT Slovenija. Dogodek je bil razdeljen v štiri segmente, v okviru katerih je obravnaval finančne spodbude in mehanizme za zagotavljanje trajnostnega prehoda, tudi z uporabo novih in inovativnih lahkih zlitin ter vodikovih in baterijsko-električnih tehnologij.
Keywords: urbana mobilnost, pametna mobilnost, trajnostna mobilnost, mesta, trajnostni razvoj, urbano okolje, surovine, lahke zlitine, vodikove tehnologije, baterijsko električne tehnologije
Published in DiRROS: 25.01.2024; Views: 152; Downloads: 74
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