20.500.12556/DiRROS-7795 Razvoj metode za HPLC-analizo izbranih fenolnih spojin lesa Development of the HPLC method for separation of some phenolic compounds of wood Preučevali smo vpliv sestave mobilne faze na ločitev enostavnih fenolnih spojin, kot so galna kislina, pirokatehol, floroglucinol, resorcinol, vanilin in flavonoida katehina s tekočinsko kromatografijo visoke ločljivosti (HPLC). Kot najbolj primerna metoda za ločitev komponent se je izkazala metoda gradientnega izpiranja s programiranim spreminjanjem sestave mobilne faze acetonitril in 2-odstotna vodna raztopina mravljinčne kisline od 2 : 98 do 25 : 75. Ločitev organskih spojin je bila učinkovitejša v mobilni fazi z manjšim deležem mravljinčne kisline. HPLC je primerna metoda za identifikacijo in kvantitativno analizo spojin v različnih tipih vzorcev na širokem področju verige gozdarstva, lesarstva in papirništva. The most suitable composition of mobile phase for the separation of simple phenolic compounds such as gallic acid, pyrocatechol, phloroglucinol, resorcinol, vanillin and related compound catechin by the high performance liquid chromatography was investigated. The most appropriate developing mobile system for the separation of these organic compounds was acetonitrile and 2% water solution of formic acid with gradient ratio from 2.98 to 25:75. The best separation of the six organic compounds was achieved using mobile phase with lower content of formic acid. HPLC is a method of choice for qualitative and quantitative analysis compounds in various types of samples in the broad field of forestry-wood-pulp-paper chain. les ekstraktivi fenolne spojine katehin kromatografija HPLC ločba true false true Slovenski jezik Angleški jezik Znanstveno delo 2017-07-12 09:19:32 2017-07-12 09:19:32 2022-08-16 09:29:29 0000-00-00 00:00:00 2015 0 0 Besedilo v slov.; str. 11-17 Vol. 108 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 630*84:547.562.4 2335-3112 URN:NBN:SI:doc-ARHB6K2T 10.20315/ASetL.108.2 4357030 266761216 RAZ_Poljansek_Ida_i2015.pdf RAZ_Poljansek_Ida_i2015.pdf 1 3D34754A489FA5B6DE0566EBD8D4B86C 12e25797c35933fc21489d86e65c93edcb8e07f9a165fe26ab9da460f6d7dad0 2aadda51-17b5-11ed-b6b8-001a4af901a5 20.500.12556/dirros/2be7c7c1-10ac-4daf-907b-c724a51b96fb https://dirros.openscience.si/Dokument.php?lang=slv&id=9660 Gozdarski inštitut Slovenije Acta Silvae et Ligni 0 0 0