20.500.12556/DiRROS-6101 Bimodal dynamics of primary metabolism-related responses in tolerant potato-Potato virus Y interaction Background Potato virus Y (PVY) is a major pathogen that causes substantial economic losses in worldwide potato production. Different potato cultivars differ in resistance to PVY, from severe susceptibility, through tolerance, to complete resistance. The aim of this study was to better define the mechanisms underlying tolerant responses of potato to infection by the particularly aggressive PVYNTN strain. We focused on the dynamics of the primary metabolism-related processes during PVYNTN infection. Results A comprehensive analysis of the dynamic changes in primary metabolism was performed, which included whole transcriptome analysis, nontargeted proteomics, and photosynthetic activity measurements in potato cv. Désirée and its transgenic counterpart depleted for accumulation of salicylic acid (NahG-Désirée). Faster multiplication of virus occurred in the NahG-Désirée, with these plants developing strong disease symptoms. We show that while the dynamics of responses at the transcriptional level are extensive and bimodal, this is only partially translated to the protein level, and to the final functional outcome. Photosynthesis-related genes are transiently induced before viral multiplication is detected and it is down-regulated later on. This is reflected as a deficiency of the photosynthetic apparatus at the onset of viral multiplication only. Interestingly, specific and constant up-regulation of some RuBisCO transcripts was detected in Désirée plants, which might be important, as these proteins have been shown to interact with viral proteins. In SA-deficient and more sensitive NahG-Désirée plants, consistent down-regulation of photosynthesis-related genes was detected. A constant reduction in the photochemical efficiency from the onset of viral multiplication was identified; in nontransgenic plants this decrease was only transient. The transient reduction in net photosynthetic rate occurred in both genotypes with the same timing, and coincided with changes in stomatal conductivity. Conclusions Down-regulation of photosynthesis-related gene expression and decreased photosynthetic activity is in line with other studies that have reported the effects of biotic stress on photosynthesis. Here, we additionally detected induction of light-reaction components in the early stages of PVYNTN infection of tolerant interaction. As some of these components have already been shown to interact with viral proteins, their overproduction might contribute to the absence of symptoms in cv. Désirée. plant-pathogen interactions Potato virus Y potyviridae salicylic acid whole transcriptome analysis shot-gun proteomics photosynthetic parameters Solanum tuberosum true false true Angleški jezik Neznan jezik Delo ni kategorizirano 2016-06-09 03:18:12 2024-07-29 10:58:32 2024-07-30 04:00:44 0000-00-00 00:00:00 2015 0 0 str. 1-17 art. no. 716 Vol. 16 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2015-09-19 1471-2164 578.2 10.1186/s12864-015-1925-2 3614031 2438420 http://dx.doi.org/10.1186/s12864-015-1925-2 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=6219 RAZ_Turnsek_Neza_2015.pdf RAZ_Turnsek_Neza_2015.pdf 1 AA7A16B1B50E6FBD93C77FC3B7856C70 d16085aabdf0c5cf44e4ada34c8547ceceac67165a4562702238f6724f75eee7 622634de-4d78-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27668 Nacionalni inštitut za biologijo 0 0 0