20.500.12556/DiRROS-6098 Rule-based design of plant expression vectors using GenoCAD Plant synthetic biology requires software tools to assist on the design of complex multi-genic expression plasmids. Here a vector design strategy to express genes in plants is formalized and implemented as a grammar in GenoCAD, a Computer-Aided Design software for synthetic biology. It includes a library of plant biological parts organized in structural categories and a set of rules describing how to assemble these parts into large constructs. Rules developed here are organized and divided into three main subsections according to the aim of the final construct: protein localization studies, promoter analysis and protein-protein interaction experiments. The GenoCAD plant grammar guides the user through the design while allowing users to customize vectors according to their needs. Therefore the plant grammar implemented in GenoCAD will help plant biologists take advantage of methods from synthetic biology to design expression vectors supporting their research projects. plasmid construction plant genetics gene expression true false true Angleški jezik Neznan jezik Delo ni kategorizirano 2016-06-09 03:18:02 2024-07-26 13:55:30 2024-07-27 04:00:54 0000-00-00 00:00:00 2015 0 0 str. 1-10 iss. 7 Vol. 10 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2015-07-06 1932-6203 577.21 10.1371/journal.pone.0132502 3528271 2005896 http://dx.doi.org/10.1371/journal.pone.0132502 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=6216 RAZ_Coll_Rius_Anna_2015.pdf RAZ_Coll_Rius_Anna_2015.pdf 1 1184390A128D44FA737ABA836361DB3C 88834d924263271732a48520a409c47b6d3e28ee85ea3313a1001d60ca0c799f 6640a6ab-4b35-11ef-b487-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=27639 Nacionalni inštitut za biologijo 0 0 0