20.500.12556/DiRROS-5622 Design principles for rapid folding of knotted DNA nanostructures Knots are some of the most remarkable topological features in nature. Self-assembly of knotted polymers without breaking or forming covalent bonds is challenging, as the chain needs to be threaded through previously formed loops in an exactly defined order. Here we describe principles to guide the folding of highly knotted single-chain DNA nanostructures as demonstrated on a nano-sized square pyramid. Folding of knots is encoded by the arrangement of modules of different stability based on derived topological and kinetic rules. Among DNA designs composed of the same modules and encoding the same topology, only the one with the folding pathway designed according to the "free-end" rule folds efficiently into the target structure. Besides high folding yield on slow annealing, this design also folds rapidly on temperature quenching and dilution from chemical denaturant. This strategy could be used to design folding of other knotted programmable polymers such as RNA or proteins. biokemija DNA nanostrukture struktura snovi true false true Angleški jezik Ni določen Članek v reviji 2016-04-13 10:23:51 2016-04-13 10:23:51 2022-08-10 06:06:44 0000-00-00 00:00:00 2016 0 0 Sodelavci: John S. Schreck, Slavko Čeru, Helena Gradišar, Nino Bašić, Tomaž Pisanski, Jonathan P. K. Doye & Roman Jerala; Opis vira z dne 7. 3. 2016; Nasl. z nasl. zaslona; Št. članka 10803; str. 1-8 Vol. 7 Feb. 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.2 2041-1723 10.1038/ncomms10803 5880858 2315876 RAZ_Kocar_Vid_i2016.pdf RAZ_Kocar_Vid_i2016.pdf 1 1DAE78AF2A52BB0131C29E726A2241E9 c449f32f42adc748fbe24fa5cc7dd92217bf5da3de7d537b98a602b447bf5d95 ebd6907e-17b6-11ed-b6b8-001a4af901a5 20.500.12556/dirros/5b23d4fd-014f-4351-8361-2e98365fbd10 https://dirros.openscience.si/Dokument.php?lang=slv&id=19802 http://dx.doi.org/10.1038/ncomms10803 1 https://dirros.openscience.si/Dokument.php?lang=slv&id=5722 Kemijski inštitut 0 0 0