20.500.12556/DiRROS-31917 Developing a durable, memory-driven, CspZ-targeting Lyme disease vaccine by rationale adjuvant selection Rational adjuvant selection is a systematic approach based on adjuvant-mediated immunomodulation to identify safe vaccine regimens that enhance protective immunity. Transmitted through ticks and caused by the bacterium Borrelia burgdorferi (Bb), Lyme disease (LD) is the most common vector-borne disease in the Northern hemisphere. There are no approved human vaccines, making it suitable for testing the concept of rational adjuvant selection. Here, we formulated our previously developed and effective LD vaccine antigen, CspZ-YAC187S, with different adjuvants suitable for human use; we analyzed the immune response by transcriptomics and tested the vaccine efficacy after Bb infection. We identified Alum-CpG and Alum-αGal to elicit the highest titers of CspZ-YAC187S-dependent protective antibodies and robust levels of protection, but through distinct mechanisms of immunomodulation. We demonstrated that immunization with Alum-CpG formulated CspZ-YAC187S provided up to nine months of protective bactericidal antibody titers, as well as recall-memory response to prevent LD after natural infection. Immunity was linked to elevated levels of IgG1 memory cells in the vaccine-triggered immune responses. This work thus identified a durable, memory immunity-driven LD vaccine, ultimately paving the road to understand the mechanisms of rationale adjuvant selection for vaccine development. Lyme disease Lyme disease vaccine immunization true false true Angleški jezik Ni določen Neznano 2026-08-18 13:42:44 2026-08-18 13:42:45 2026-08-19 03:46:21 0000-00-00 00:00:00 2026 0 0 Nasl. z nasl. zaslona; Opis vira z dne 29. 5. 2026; str. 1-15 article no. ǂ168 Vol. 11 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.9 2059-0105 10.1038/s41541-026-01482-0 279895043 RAZ_McCarty_Miranda_2026.pdf RAZ_McCarty_Miranda_2026.pdf 1 FA1854E1DBFB4DC086339B4A21BC11A6 7b44a68cf51c2de4be3df3a83ee360286a950cd27a444a464f40ddacfb6aa4d6 650a24bf-9afa-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49655 https://www.nature.com/articles/s41541-026-01482-0 1 f074ddef-9af9-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49654 Univerzitetni klinični center Ljubljana 0 0 0