20.500.12556/DiRROS-31892 Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events Plant genetic engineering represents an important aspect of modern agriculture, and new genetically modified (GM) crop varieties are entering the market on a regular basis. This necessitates the development of high throughput multi-target analytical methods to detect and quantify their presence for regulatory compliance. In this study, we present a multiplex dPCR method for discriminative quantification of 19 GM soybean events and the lectin (Le1) endogene on a nanowell plate-based all-in-one dPCR system. The method consists of four 5-plex assays, taking advantage of the platform’s multiple fluorescence detection channels. The assays complied with the minimum performance requirements in terms of specificity, trueness, precision, sensitivity and dynamic range, making them suitable for use in routine detection and quantification of GM crops. This method represents the most comprehensive multi-target GM soybean quantification approach to date without the need for prior screening and features a simplified workflow, making it suitable for widespread adoption. Our study sets a precedent for rapid and straightforward development of multiplex dPCR GM crop quantification assays to address the evolving demands of regulatory monitoring. genetically modified (GM) crops transgenic soybean glycine max (L.) merr quantification digital PCR multiplex multi-target true false false Taylor & Francis Group Angleški jezik Slovenski jezik Delo ni kategorizirano 2026-08-17 08:03:55 2026-08-17 08:15:46 2026-09-01 08:13:35 0000-00-00 00:00:00 2026 0 0 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-02-28 10.6084/m9.figshare.31436110 RAZ_Jelencic_Amadej_2026.zip RAZ_Jelencic_Amadej_2026.zip 1 26E7D030EDA4BDAD7E7A3D8C987022EF 5a4a5262216351eb658666166ba3b9639bd3a521aa08e04c024ec309c61792c4 5bd4984a-9a02-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49583 https://doi.org/10.6084/m9.figshare.31436110 0 49fd16bf-9a02-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49582 31436110.xml 31436110.xml 1 0B4AABA1F25FE01D7E4FE40E40B4ECAA 75586048eda158289641bd5097f7815bbd77d5f0b3b1dedf6a26aa0481a43f46 789b94f2-9a02-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49585 https://doi.org/10.1080/21645698.2026.2635816 Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events (znanstveni članek) Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events (research article) 0 ab00bb83-9a02-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49586 Nacionalni inštitut za biologijo Simple, fast, reliable: multiplex digital PCR quantification of 19 genetically modified soybean events 0 0 0