20.500.12556/DiRROS-31707 Integrative transcriptomic profiling of the Wilms tumor Our study aimed to identify relevant transcriptomic biomarkers for the Wilms tumor, the most common pediatric kidney cancer, independent of the histological type and stage. Using next-generation sequencing, we analyzed the miRNA profiles of 74 kidney samples, which were divided into two independent groups: fresh frozen tissue and formalin-fixed paraffin-embedded tissue samples. Subsequent mRNA expression profiling and pathway analysis were performed to establish the interplay and potential involvement of miRNAs and mRNA in the Wilms tumor. Comparative analysis, irrespective of post-dissection tissue processing, revealed 41 differentially expressed miRNAs, with 27 miRNAs having decreased expression and 14 miRNAs having increased expression in the Wilms tumor tissue compared to healthy kidney tissue. Among global mRNA transcriptomic profile differences, cross-sectional analysis suggested a limited list of genes potentially regulated by differentially expressed miRNAs in the Wilms tumor. This study identified the comprehensive miRNA and mRNA profile of the Wilms tumor using next-generation sequencing and bioinformatics approach, providing better insights into the pathogenesis of the Wilms tumor. The identified Wilms tumor miRNAs have potential as biomarkers for the diagnosis and treatment of the Wilms tumor, regardless of histological subtype and disease stage. Wilms tumor NGS miRNA profile mRNA profile universal biomarkers Wilmsov tumor profil mRNA profil miRNA univerzalni biološki označevalci true false true Angleški jezik Slovenski jezik Neznano 2026-08-06 10:33:37 2026-08-06 10:33:37 2026-08-07 03:58:32 0000-00-00 00:00:00 2023 0 0 Nasl. z nasl. zaslona; Opis vira z dne 21. 9. 2023; str. 1-19 iss. 15, [article no.] 3846 Vol. 15 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2072-6694 10.3390/cancers15153846 165310723 RAZ_Avcin_Simona_Lucija_2023.pdf RAZ_Avcin_Simona_Lucija_2023.pdf 1 CB60E4A4D0732D693B96712F92938935 1b516cf45b3e135c90e528f12ba67e4a75a5f2fc2b7c8203b62e97e371b538d3 c2ba9265-9171-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49216 https://www.mdpi.com/2072-6694/15/15/3846 1 85c385c9-9171-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49214 Univerzitetni klinični center Ljubljana 0 0 0