20.500.12556/DiRROS-31702 A dual biomarker TK1 protein and CA125 or HE4-based algorithm as a better diagnostic tool than ROMA Index in early detection of ovarian cancer Background: The early detection of ovarian cancer is presently not effective, and it is crucial to establish biomarkers for the early diagnosis of ovarian cancer to improve the survival of patients. Materials and methods: The aim of this study was to investigate the role of thymidine kinase 1 (TK1) in combination with CA 125 or HE4 to serve as a potential diagnostic biomarkers for ovarian cancer. In this study, a set of 198 serum samples consisting of 134 ovarian tumor patients and 64 healthy age-matched controls were analyzed. The TK1 protein levels in serum samples were determined using the AroCell TK 210 ELISA. Results: A combination of TK1 protein with CA 125 or HE4 showed better performance than either of them alone in the differentiation of early stage ovarian cancer from the healthy control group, but also a significantly better performance than the ROMA index. However, this was not observed using a TK1 activity test in combination with the other markers. Furthermore, the combination of TK1 protein and CA 125 or HE4 could differentiate early stage disease (stage I, II) more efficiently from advanced-stage (stage III, IV) disease (p < 0.0001). Conclusions: The combination of TK1 protein with CA 125 or HE4 increased the potential of detecting ovarian cancer at early stages. thymidine kinase 1 TK 210 ELISA TK-liaison CA 125 HE4 ROMA index ovarian cancer timidin kinaza 1 indeks ROMA true false true Angleški jezik Slovenski jezik Neznano 2026-08-06 10:07:04 2026-08-06 10:07:04 2026-08-07 03:58:32 0000-00-00 00:00:00 2023 0 0 Nasl. z nasl. zaslona; Opis vira z dne 6. 3. 2023; 18 str. iss. 5, art. 1593 Vol. 15 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 612.621:616-006 2072-6694 10.3390/cancers15051593 144061955 RAZ_Cviic_Diana_2023.pdf RAZ_Cviic_Diana_2023.pdf 1 FB391B7094974A69D801CBDE05A68A1C 748c81847c887d9a8d6b56e7c965554093003b32fbdd400a04dd6ae2ed12a0cd e6bc2bce-916d-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49206 https://www.mdpi.com/2072-6694/15/5/1593 1 d10901c4-916d-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49205 Univerzitetni klinični center Ljubljana 0 0 0