20.500.12556/DiRROS-31684 Cytopathological assessment is an accurate method for identifying immunophenotypic features and BRCA1/2 mutations of high-grade serous carcinoma from ascites High‐grade serous carcinoma (HGSC) is the most common and aggressive type of ovarian cancer, and it is often associated with ascites at presentation. The objective of this study was to evaluate the accuracy of cytopathology to identify immunophenotypic features of HGSC and BRCA1/2 mutations from ascites. Methods: The study included 45 patients with histologically confirmed primary HGSC and malignant ascites. Immunocytochemistry (ICC) staining for PAX8, WT1, P53, P16, and Ki‐67 was performed on cytospins and cytoblocks prepared from ascites. Next‐generation sequencing (NGS) was used to detect germline/somatic BRCA1/2 mutations in the ascites. Both ICC and NGS results were compared with immunohistochemistry (IHC) and NGS results from tissue blocks of primary tumor. Cronbach α and χ2 statistics, respectively, were used. Results: ICC/IHC results for PAX8, WT1, P53, and P16 showed good reliability between cytospins, cytoblocks, and tissue blocks (α > 0.75), whereas poor reliability and significant differences were observed for Ki‐67 between ascites and tissue blocks (α < 0.26; p < .001 [Kruskal–Wallis]). For germline BRCA1/2 mutations, 100% concordance was confirmed, but only 14% concordance was confirmed for somatic mutations. Conclusions: These results demonstrate that cytopathology is an accurate method for identifying immunophenotypic features of HGSC and detecting germline BRCA1/ 2 mutations from ascites. However, further investigation is required for assessing the proliferation activity of HGSC in ascites and for detecting somatic BRCA1/2 mutations. ascites BRCA1/2 mutations cytopathology high-grade serous carcinoma ascites mutacije BRCA 1/2 citopatologija visoko maligen rak true false true Angleški jezik Slovenski jezik Neznano 2026-08-06 09:21:13 2026-08-06 09:21:14 2026-08-07 03:58:30 0000-00-00 00:00:00 2023 0 0 str. 188-197 iss. 3 Vol. 131 Mar. 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-07 1934-662X 10.1002/cncy.22664 131704067 RAZ_Miceska_Simona_2023.pdf RAZ_Miceska_Simona_2023.pdf 1 05DEFAB5564E106FA0A44DA4F3AB69FF 91bc9bd5782a5cf5e69be337b4177b5c7690161fb1225322b31f9722ae101cb2 86dca954-9167-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49169 https://acsjournals.onlinelibrary.wiley.com/doi/epdf/10.1002/cncy.22664 1 6913ff01-9167-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=49168 Univerzitetni klinični center Ljubljana 0 0 0