20.500.12556/DiRROS-31532 Disturbed antioxidant capacity in patients with systemic sclerosis associates with lung and gastrointestinal symptoms The correct balance between reactive oxygen species and antioxidant defense in an organism is disturbed in oxidative stress. To assess oxidative balance in 36 SSc patients and 26 healthy controls (HCs), we measured reactive oxidative metabolites (ROMs), total antioxidant capacity (TAC), lipid peroxidation (measuring 4-HNE), and DNA oxidative damage (measuring 8-OHdG) in serum. Furthermore, DNA breaks in leukocytes of 35 SSc patients and 32 HCs were evaluated using COMET. While we report high ROMs for both SSc patients and age/sex matched HC samples, there was a significant increase in TAC in SSc patients as compared to HCs, and thus also a significantly higher oxidative stress index in SSc patients. TAC was significantly higher in SSc patients with ILD and gastrointestinal involvement, as well as in patients with anti-topoisomerase antibodies. We observe no difference in serum lipid peroxidation status or oxidative DNA damage. However, SSc patients had significantly more leukocyte DNA breaks than HCs; the most damage was observed in patients treated with immunosuppressives. Thus, our study confirms presence of oxidative stress and increased DNA damage in leukocytes of SSc patients; however, it points toward increased antioxidant capacity, which needs to be further studied. systemic sclerosis oxidative stress reactive oxidative metabolites antioxidants lipid peroxidation DNA damage DNA breaks sistemska skleroza oksidativni stres reaktivni oksidativni metaboliti antioksidanti peroksidacija lipidov poškodbe DNK DNK zlomi true false true Angleški jezik Slovenski jezik Neznano 2026-08-05 09:52:39 2026-08-05 09:52:39 2026-08-06 04:01:28 0000-00-00 00:00:00 2023 0 0 Nasl. z nasl. zaslona; Opis vira z dne 28. 7. 2023; str. 1-16 iss. 8, [article no.] 2110 Vol. 11 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-002 2227-9059 10.3390/biomedicines11082110 159972611 RAZ_Brezovec_Neza_2023.pdf RAZ_Brezovec_Neza_2023.pdf 1 9EAE0E20C7605C0BD64CA8C85CB00651 07e1444e898ddac4d8a5d7134b25727d8be546c44509099e1bb4a6060ef9accf d5af25d4-90a2-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=48874 https://www.mdpi.com/2227-9059/11/8/2110 1 a2d633e5-90a2-11f1-af5a-001a4af901a5 https://dirros.openscience.si/Dokument.php?lang=slv&id=48873 Univerzitetni klinični center Ljubljana 0 0 0